##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AflIII Aor51HI ApaI AscI AspI
AviII BciVI BfrI BlpI Bpu1102I
BseAI BsiEI BsiWI BspEI BspLU11I
BsrBI BssHII BstAPI BstBI BstEII
BstPI BstUI BstXI CelII CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FauI FseI
FspI HgaI KspI MfeI MluI
MroI MspA1I MunI MvnI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmeI PshAI Psp1406I PvuI
PvuII RsrII SacII SalI SbfI
SexAI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 4636 GGTACC G^GTACC
AcyI 1373 GGCGCC G[GA]^CG[CT]C
AgeI 122 ACCGGT A^CCGGT
AocI 440 CCTGAGG CC^T[ACGT]AGG
Asp718I 4636 GGTACC G^GTACC
AvaI 4931 CCCGAG C^[CT]CG[GA]G
AvrII 4641 CCTAGG C^CTAGG
BalI 5013 TGGCCA TGG^CCA
BbeI 1373 GGCGCC GGCGC^C
BbrPI 3398 CACGTG CAC^GTG
BbvCI 367 GCTGAGG CCTCAGC(-5/-2)
BcgI 347 CGAGAACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3081 TGATCA T^GATCA
BglI 62 GCCCTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4641 CCTAGG C^CTAGG
BsaAI 3398 CACGTG [CT]AC^GT[GA]
BsaBI 4470 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1373 GGCGCC G[GA]^CG[CT]C
BsaWI 122 ACCGGT [AT]^CCGG[AT]
BsmI 1738 GAATGC GAATGC(1/-1)
BsoBI 4931 CCCGAG C^[CT]CG[GA]G
Bsp1407I 5412 TGTACA T^GTACA
BspDI 2295 ATCGAT AT^CGAT
BspHI 3222 TCATGA T^CATGA
BsrGI 5412 TGTACA T^GTACA
BssSI 822 CTCGTG CACGAG(-5/-1)
Bst1107I 4554 GTATAC GTA^TAC
BstZ17I 4554 GTATAC GTA^TAC
Bsu36I 440 CCTGAGG CC^T[ACGT]AGG
CfoI 1374 GCGC GCG^C
ClaI 2295 ATCGAT AT^CGAT
DraIII 1309 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1078 CCGTGG C^C[GA][CT]GG
Eco81I 440 CCTGAGG CC^T[ACGT]AGG
FbaI 3081 TGATCA T^GATCA
HaeII 1373 GGCGCC [GA]GCGC^[CT]
HhaI 1374 GCGC GCG^C
Hin1I 1373 GGCGCC G[GA]^CG[CT]C
HinP1I 1374 GCGC G^CGC
HpaI 3073 GTTAAC GTT^AAC
KasI 1373 GGCGCC G^GCGCC
KpnI 4636 GGTACC GGTAC^C
MamI 4470 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 5013 TGGCCA TGG^CCA
MscI 5013 TGGCCA TGG^CCA
NarI 1373 GGCGCC GG^CGCC
NspI 3445 GCATGC [GA]CATG^[CT]
PinAI 122 ACCGGT A^CCGGT
PmaCI 3398 CACGTG CAC^GTG
PmlI 3398 CACGTG CAC^GTG
PstI 2247 CTGCAG CTGCA^G
RcaI 3222 TCATGA T^CATGA
SapI 845 GAAGAGC GCTCTTC(1/4)
ScaI 285 AGTACT AGT^ACT
SfiI 61 GGCCCTGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1373 GGCGCC GGC^GCC
SphI 3445 GCATGC GCATG^C
SspBI 5412 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 72 CCGC CCGC(-3/-1)
4893 CCGC
AhdI 313 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
589 GACGGTACGTC
Alw44I 1306 GTGCAC G^TGCAC
4018 GTGCAC
ApaLI 1306 GTGCAC G^TGCAC
4018 GTGCAC
AseI 4271 ATTAAT AT^TAAT
5443 ATTAAT
AsnI 4271 ATTAAT AT^TAAT
5443 ATTAAT
AspEI 313 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
589 GACGGTACGTC
BamHI 1026 GGATCC G^GATCC
2413 GGATCC
BanI 1373 GGCGCC G^G[CT][GA]CC
4636 GGTACC
BcnI 2 CCGGG CC^[GC]GG
5100 CCCGG
BglII 106 AGATCT A^GATCT
1951 AGATCT
BsgI 4032 CTGCAC GTGCAG(16/14)
461 GTGCAG
BsrDI 2593 CATTGC GCAATG(2/0)
4862 GCAATG
EaeI 1162 TGGCCG [CT]^GGCC[GA]
5013 TGGCCA
Eam1105I 313 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
589 GACGGTACGTC
HincII 3073 GTTAAC GT[CT]^[GA]AC
5269 GTCAAC
HindII 3073 GTTAAC GT[CT]^[GA]AC
5269 GTCAAC
NciI 2 CCGGG CC^[GC]GG
5100 CCCGG
PflMI 476 CCAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2274 CCAGAATGTGG
PshBI 4271 ATTAAT AT^TAAT
5443 ATTAAT
SacI 446 GAGCTC GAGCT^C
1912 GAGCTC
SspI 2968 AATATT AAT^ATT
3116 AATATT
Van91I 476 CCAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2274 CCAGAATGTGG
XcmI 307 CCAGGAGACATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3841 CCATAAGGAATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr10I Cfr13I DdeI DpnII DraI
DraII EarI EcoNI EcoO109I EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI