##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AvaI AviII AvrII BanI
BanII BbeI BbrPI BbvCI BcgI
BcnI BglI BlnI BsaAI BsaBI
BsaHI BseRI BsiEI BsiWI BsoBI
Bsp1407I BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CfoI Cfr10I CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV FseI FspI
HaeII HhaI Hin1I HinP1I KasI
KpnI KspI MamI MfeI MluI
MunI MvnI NaeI NarI NciI
NgoMIV NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I SspBI
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 2838 GTAGAC GT^[AC][GT]AC
AccIII 9 TCCGGA T^CCGGA
Asp700I 2738 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 2940 GTGCTC G[AT]GC[AT]^C
BglII 4537 AGATCT A^GATCT
BlpI 1862 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1862 GCTTAGC GC^T[ACGT]AGC
BsaI 1969 GAGACC GGTCTC(1/5)
BsaWI 9 TCCGGA [AT]^CCGG[AT]
BseAI 9 TCCGGA T^CCGGA
BsgI 19 CTGCAC GTGCAG(16/14)
BsiHKAI 2940 GTGCTC G[AT]GC[AT]^C
BsmBI 3449 CGTCTC CGTCTC(1/5)
BspDI 1442 ATCGAT AT^CGAT
BspEI 9 TCCGGA T^CCGGA
CelII 1862 GCTTAGC GC^T[ACGT]AGC
ClaI 1442 ATCGAT AT^CGAT
DraII 4007 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 1032 CCATGG C^C[GA][CT]GG
EcoO109I 4007 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT22I 3624 ATGCAT ATGCA^T
FauI 1321 GCGGG CCCGC(4/6)
HapII 10 CCGG C^CGG
HgaI 105 GACGC GACGC(5/10)
HpaI 3264 GTTAAC GTT^AAC
HpaII 10 CCGG C^CGG
MroI 9 TCCGGA T^CCGGA
MspI 10 CCGG C^CGG
NcoI 1032 CCATGG C^CATGG
NheI 3716 GCTAGC G^CTAGC
NsiI 3624 ATGCAT ATGCA^T
PflMI 1869 CCAGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 4007 GGGACCT [GA]G^G[AT]CC[CT]
ScaI 3913 AGTACT AGT^ACT
SpeI 2688 ACTAGT A^CTAGT
SphI 3622 GCATGC GCATG^C
StuI 3697 AGGCCT AGG^CCT
TaqI 1443 TCGA T^CGA
TthHB8I 1443 TCGA T^CGA
Van91I 1869 CCAGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4023 TCTAGA T^CTAGA
XcmI 3044 CCAGAACACTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2738 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflII 4142 CTTAAG C^TTAAG
4578 CTTAAG
AlwNI 103 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
4216 CAGCTGCTG
AocI 344 CCTCAGG CC^T[ACGT]AGG
509 CCTTAGG
BalI 1925 TGGCCA TGG^CCA
2809 TGGCCA
BciVI 504 GGATAC GTATCC(6/5)
3588 GTATCC
BfrI 4142 CTTAAG C^TTAAG
4578 CTTAAG
BmyI 2940 GTGCTC G[AGT]GC[ACT]^C
3367 GGGCAC
Bsp1286I 2940 GTGCTC G[AGT]GC[ACT]^C
3367 GGGCAC
BspMI 1671 ACCTGC ACCTGC(4/8)
2154 ACCTGC
BstXI 156 CCAAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
353 CCAGTCCTTTGG
Bsu36I 344 CCTCAGG CC^T[ACGT]AGG
509 CCTTAGG
EaeI 1925 TGGCCA [CT]^GGCC[GA]
2809 TGGCCA
Eco81I 344 CCTCAGG CC^T[ACGT]AGG
509 CCTTAGG
HincII 2505 GTTGAC GT[CT]^[GA]AC
3264 GTTAAC
HindII 2505 GTTGAC GT[CT]^[GA]AC
3264 GTTAAC
HindIII 279 AAGCTT A^AGCTT
2495 AAGCTT
MluNI 1925 TGGCCA TGG^CCA
2809 TGGCCA
MscI 1925 TGGCCA TGG^CCA
2809 TGGCCA
PstI 668 CTGCAG CTGCA^G
2174 CTGCAG
PvuII 4032 CAGCTG CAG^CTG
4216 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI AvaII BamHI
BbsI BbvI BclI BfaI BpmI
BpuAI BsaJI BsiYI BslI BsmAI
BsmFI BsmI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraIII EarI
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MvaI MwoI NdeI NdeII NlaIII
NlaIV NspI PleI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII