##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AatII AccI AflII AgeI Alw44I
Aor51HI ApaLI AscI AvrII BbeI
BfrI BglI BlnI BsaBI BspDI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI Eco47III EcoT22I HindIII HpaI
KasI KspI MamI MluI NarI
NheI NotI NsiI NspV PacI
PinAI PmeI PshAI PvuI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SpeI Sse8387I SwaI XcmI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
Acc65I 4430 GGTACC G^GTACC
AccIII 1338 TCCGGA T^CCGGA
AclI 1751 AACGTT AA^CGTT
AcyI 2733 GACGCC G[GA]^CG[CT]C
AhdI 1981 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1035 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 22 GGGCCC GGGCC^C
AseI 3314 ATTAAT AT^TAAT
AsnI 3314 ATTAAT AT^TAAT
Asp718I 4430 GGTACC G^GTACC
AspEI 1981 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 16 TGCGCA TGC^GCA
BalI 2050 TGGCCA TGG^CCA
BamHI 2347 GGATCC G^GATCC
BbrPI 4456 CACGTG CAC^GTG
BciVI 2746 GTATCC GTATCC(6/5)
BglII 3952 AGATCT A^GATCT
BlpI 4197 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4197 GCTGAGC GC^T[ACGT]AGC
BsaHI 2733 GACGCC G[GA]^CG[CT]C
BseAI 1338 TCCGGA T^CCGGA
BsiEI 1592 CGGCCG CG[GA][CT]^CG
BsiWI 1457 CGTACG C^GTACG
BsmBI 424 GAGACG CGTCTC(1/5)
BsmI 2657 GCATTC GAATGC(1/-1)
BspEI 1338 TCCGGA T^CCGGA
BspHI 4088 TCATGA T^CATGA
BspLU11I 3779 ACATGT A^CATGT
BssSI 421 CACGAG CACGAG(-5/-1)
BstEII 104 GGTTACC G^GT[ACGT]ACC
BstPI 104 GGTTACC G^GT[ACGT]ACC
CelII 4197 GCTGAGC GC^T[ACGT]AGC
EagI 1592 CGGCCG C^GGCCG
Eam1105I 1981 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1592 CGGCCG C^GGCCG
Eco52I 1592 CGGCCG C^GGCCG
EcoO65I 104 GGTTACC G^GT[ACGT]ACC
EcoRI 1732 GAATTC G^AATTC
EcoRV 1899 GATATC GAT^ATC
FseI 1593 GGCCGGCC GGCCGG^CC
FspI 16 TGCGCA TGC^GCA
Hin1I 2733 GACGCC G[GA]^CG[CT]C
KpnI 4430 GGTACC GGTAC^C
MfeI 4153 CAATTG C^AATTG
MluNI 2050 TGGCCA TGG^CCA
MroI 1338 TCCGGA T^CCGGA
MscI 2050 TGGCCA TGG^CCA
MunI 4153 CAATTG C^AATTG
NruI 2778 TCGCGA TCG^CGA
PaeR7I 541 CTCGAG C^TCGAG
PmaCI 4456 CACGTG CAC^GTG
PmlI 4456 CACGTG CAC^GTG
PpuMI 4662 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 3314 ATTAAT AT^TAAT
Psp1406I 1751 AACGTT AA^CGTT
RcaI 4088 TCATGA T^CATGA
SapI 224 GCTCTTC GCTCTTC(1/4)
SgrAI 858 CACCGGCG C[GA]^CCGG[CT]G
SnaBI 270 TACGTA TAC^GTA
SphI 4307 GCATGC GCATG^C
XbaI 185 TCTAGA T^CTAGA
XhoI 541 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 62 CGCG CG^CG
2779 CGCG
AspI 2487 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4559 GACCCTGTC
BbsI 891 GAAGAC GAAGAC(2/6)
1791 GAAGAC
BbvCI 1400 CCTCAGC CCTCAGC(-5/-2)
2826 CCTCAGC
BcgI 1154 CGACGCAGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4474 GCAGAATTGTCG
BpuAI 891 GAAGAC GAAGAC(2/6)
1791 GAAGAC
BsaAI 270 TACGTA [CT]AC^GT[GA]
4456 CACGTG
BsaI 127 GAGACC GGTCTC(1/5)
942 GAGACC
BsrDI 4470 CATTGC GCAATG(2/0)
2846 GCAATG
BstAPI 2991 GCACCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3024 GCAAGTTCTGC
BstUI 62 CGCG CG^CG
2779 CGCG
BstXI 94 CCAGGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1560 CCACCCGGCTGG
DraI 1685 TTTAAA TTT^AAA
3357 TTTAAA
DrdI 523 GACCAAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4525 GACCCTGCTGTC
MslI 1737 CACTTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2948 CATGGCCGTG
MvnI 62 CGCG CG^CG
2779 CGCG
NaeI 1387 GCCGGC GCC^GGC
1594 GCCGGC
NcoI 484 CCATGG C^CATGG
4721 CCATGG
NdeI 1665 CATATG CA^TATG
2911 CATATG
NgoMIV 1387 GCCGGC G^CCGGC
1594 GCCGGC
PflFI 2487 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4559 GACCCTGTC
PvuII 2829 CAGCTG CAG^CTG
3086 CAGCTG
SexAI 3151 ACCTGGT A^CC[AT]GGT
4842 ACCTGGT
SmaI 2 CCCGGG CCC^GGG
711 CCCGGG
StuI 2159 AGGCCT AGG^CCT
4766 AGGCCT
Tth111I 2487 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4559 GACCCTGTC
XmaCI 2 CCCGGG C^CCGGG
711 CCCGGG
XmaI 2 CCCGGG C^CCGGG
711 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AocI ApoI Asp700I AspHI
AvaI AvaII BanI BanII BbvI
BclI BcnI BfaI BmyI BpmI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrBI BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
RsaI SacI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI