##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AccIII AflII Aor51HI
AscI Asp718I AviII BamHI BbeI
BfrI BglII BseAI BsiWI BspDI
BspEI BssHII Bst1107I BstEII BstPI
BstZ17I ClaI CpoI DrdI Eco47III
EcoO65I EcoRV EcoT22I FseI FspI
KasI KpnI KspI MluI MroI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PaeR7I PflMI
PpuMI PvuI RsrII SacII SalI
SbfI SfiI SfoI SgrAI SmaI
SnaBI Sse8387I SwaI Van91I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AccI 4191 GTCTAC GT^[AC][GT]AC
AccII 1 CGCG CG^CG
AclI 2811 AACGTT AA^CGTT
AcyI 4827 GACGCC G[GA]^CG[CT]C
AgeI 54 ACCGGT A^CCGGT
AhdI 4725 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2071 GTGCAC G^TGCAC
AlwNI 2173 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1042 CCTGAGG CC^T[ACGT]AGG
ApaLI 2071 GTGCAC G^TGCAC
AseI 3702 ATTAAT AT^TAAT
AsnI 3702 ATTAAT AT^TAAT
AspEI 4725 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 5541 CCTAGG C^CTAGG
BanI 598 GGTGCC G^G[CT][GA]CC
BbsI 656 GAAGAC GAAGAC(2/6)
BbvCI 5503 GCTGAGG CCTCAGC(-5/-2)
BcgI 172 GCAGAAACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 431 GTATCC GTATCC(6/5)
BlnI 5541 CCTAGG C^CTAGG
BpuAI 656 GAAGAC GAAGAC(2/6)
BsaHI 4827 GACGCC G[GA]^CG[CT]C
BsiEI 1210 CGGCCG CG[GA][CT]^CG
BspHI 3086 TCATGA T^CATGA
BspLU11I 4639 ACATGT A^CATGT
BsrFI 54 ACCGGT [GA]^CCGG[CT]
BssSI 4890 CACGAG CACGAG(-5/-1)
BstBI 4388 TTCGAA TT^CGAA
BstUI 1 CGCG CG^CG
BstXI 4859 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1042 CCTGAGG CC^T[ACGT]AGG
Cfr10I 54 ACCGGT [GA]^CCGG[CT]
DsaI 993 CCATGG C^C[GA][CT]GG
EagI 1210 CGGCCG C^GGCCG
Eam1105I 4725 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1210 CGGCCG C^GGCCG
Eco52I 1210 CGGCCG C^GGCCG
Eco81I 1042 CCTGAGG CC^T[ACGT]AGG
EcoNI 528 CCTGGTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 1028 AGCGCC [GA]GCGC^[CT]
Hin1I 4827 GACGCC G[GA]^CG[CT]C
HincII 2848 GTTAAC GT[CT]^[GA]AC
HindII 2848 GTTAAC GT[CT]^[GA]AC
HpaI 2848 GTTAAC GTT^AAC
MfeI 3721 CAATTG C^AATTG
MunI 3721 CAATTG C^AATTG
MvnI 1 CGCG CG^CG
NcoI 993 CCATGG C^CATGG
NspV 4388 TTCGAA TT^CGAA
PacI 3006 TTAATTAA TTAAT^TAA
PinAI 54 ACCGGT A^CCGGT
PmeI 3998 GTTTAAAC GTTT^AAAC
PshAI 1289 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3702 ATTAAT AT^TAAT
Psp1406I 2811 AACGTT AA^CGTT
RcaI 3086 TCATGA T^CATGA
SapI 2292 GCTCTTC GCTCTTC(1/4)
SexAI 527 ACCTGGT A^CC[AT]GGT
SfuI 4388 TTCGAA TT^CGAA
SphI 1716 GCATGC GCATG^C
StuI 3927 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AspI 3310 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5097 GACTCCGTC
BbrPI 2074 CACGTG CAC^GTG
2221 CACGTG
BclI 1308 TGATCA T^GATCA
2078 TGATCA
BsaBI 3886 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5559 GATGCTTATC
BsaI 120 GGTCTC GGTCTC(1/5)
3315 GGTCTC
BsaWI 54 ACCGGT [AT]^CCGG[AT]
1383 ACCGGA
BsmBI 5102 CGTCTC CGTCTC(1/5)
3308 GAGACG
BsmI 1387 GAATGC GAATGC(1/-1)
1610 GCATTC
BsrBI 92 GAGCGG CCGCTC(-3/-3)
1207 GAGCGG
FbaI 1308 TGATCA T^GATCA
2078 TGATCA
HgaI 217 GACGC GACGC(5/10)
4827 GACGC
MamI 3886 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5559 GATGCTTATC
PflFI 3310 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5097 GACTCCGTC
PmaCI 2074 CACGTG CAC^GTG
2221 CACGTG
PmlI 2074 CACGTG CAC^GTG
2221 CACGTG
PvuII 2182 CAGCTG CAG^CTG
4728 CAGCTG
SacI 2137 GAGCTC GAGCT^C
5378 GAGCTC
ScaI 2155 AGTACT AGT^ACT
5161 AGTACT
SpeI 3257 ACTAGT A^CTAGT
3646 ACTAGT
Tth111I 3310 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5097 GACTCCGTC
XcmI 392 CCATTTATATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2090 CCAAGGAAACACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApaI ApoI Asp700I AspHI
AvaI AvaII BalI BanII BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrDI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII EaeI EarI EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI