##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
Acc65I AccI AclI AflII AflIII
AgeI Alw44I ApaLI AscI AseI
AsnI Asp718I AvrII BamHI BbrPI
BciVI BclI BfrI BlnI BsaAI
BsaBI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI Bst1107I BstZ17I
ClaI CpoI DraIII EcoRV EcoT22I
FbaI FseI KpnI MamI MfeI
MluI MunI NcoI NdeI NotI
NsiI PacI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SalI SgrAI SnaBI SpeI
SphI SspBI SspI SwaI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AatII 3047 GACGTC GACGT^C
AhdI 1914 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1914 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BglII 6147 AGATCT A^GATCT
BsmBI 96 GAGACG CGTCTC(1/5)
BstBI 5710 TTCGAA TT^CGAA
BstEII 485 GGTGACC G^GT[ACGT]ACC
BstPI 485 GGTGACC G^GT[ACGT]ACC
DraI 6272 TTTAAA TTT^AAA
DrdI 2921 GACGGCGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1211 CGGCCG C^GGCCG
Eam1105I 1914 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1211 CGGCCG C^GGCCG
Eco52I 1211 CGGCCG C^GGCCG
EcoO65I 485 GGTGACC G^GT[ACGT]ACC
EcoRI 4245 GAATTC G^AATTC
HpaI 6232 GTTAAC GTT^AAC
MslI 5785 CACCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NruI 3167 TCGCGA TCG^CGA
NspV 5710 TTCGAA TT^CGAA
PshAI 3156 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 5000 AGTACT AGT^ACT
SexAI 4507 ACCAGGT A^CC[AT]GGT
SfuI 5710 TTCGAA TT^CGAA
XbaI 2597 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 300 TCCGGA T^CCGGA
3784 TCCGGA
AcsI 4245 GAATTC [GA]^AATT[CT]
6182 AAATTC
AocI 1064 CCTAAGG CC^T[ACGT]AGG
5339 CCTCAGG
ApoI 4245 GAATTC [GA]^AATT[CT]
6182 AAATTC
Asp700I 647 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3177 GAAGAGCTTC
AspI 1181 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2387 GACCCTGTC
AviII 5546 TGCGCA TGC^GCA
5590 TGCGCA
BbsI 2918 GAAGAC GAAGAC(2/6)
3163 GTCTTC
BcgI 1943 CGAGAGCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5702 GCAGGAGCTTCG
BpuAI 2918 GAAGAC GAAGAC(2/6)
3163 GTCTTC
BseAI 300 TCCGGA T^CCGGA
3784 TCCGGA
BsiEI 1211 CGGCCG CG[GA][CT]^CG
4046 CGGTCG
BsmI 413 GAATGC GAATGC(1/-1)
4149 GAATGC
BspEI 300 TCCGGA T^CCGGA
3784 TCCGGA
Bsu36I 1064 CCTAAGG CC^T[ACGT]AGG
5339 CCTCAGG
Eco81I 1064 CCTAAGG CC^T[ACGT]AGG
5339 CCTCAGG
FspI 5546 TGCGCA TGC^GCA
5590 TGCGCA
HincII 963 GTCAAC GT[CT]^[GA]AC
6232 GTTAAC
HindII 963 GTCAAC GT[CT]^[GA]AC
6232 GTTAAC
MaeII 3048 ACGT A^CGT
5085 ACGT
MroI 300 TCCGGA T^CCGGA
3784 TCCGGA
NaeI 2020 GCCGGC GCC^GGC
3010 GCCGGC
NgoMIV 2020 GCCGGC G^CCGGC
3010 GCCGGC
NheI 2213 GCTAGC G^CTAGC
2327 GCTAGC
PaeR7I 1866 CTCGAG C^TCGAG
4358 CTCGAG
PflFI 1181 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2387 GACCCTGTC
SmaI 4093 CCCGGG CCC^GGG
4942 CCCGGG
Tsp509I 4246 AATT ^AATT
6183 AATT
Tth111I 1181 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2387 GACCCTGTC
XhoI 1866 CTCGAG C^TCGAG
4358 CTCGAG
XmaCI 4093 CCCGGG C^CCGGG
4942 CCCGGG
XmaI 4093 CCCGGG C^CCGGG
4942 CCCGGG
XmnI 647 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3177 GAAGAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Aor51HI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssHII BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco47III EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HindIII HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SacII SapI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SfiI SfoI SmlI Sse8387I StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XhoII