##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccIII AcyI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
AspEI AspI AviII BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BsaHI BsaWI BseAI
BsiWI BspDI BspEI BspLU11I BssHII
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KspI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshBI
PvuI RsrII SacII SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI StuI
SwaI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 2258 GGTACC G^GTACC
AccI 235 GTCTAC GT^[AC][GT]AC
AclI 3359 AACGTT AA^CGTT
AcsI 1305 AAATTC [GA]^AATT[CT]
Alw44I 1993 GTGCAC G^TGCAC
ApaI 841 GGGCCC GGGCC^C
ApaLI 1993 GTGCAC G^TGCAC
ApoI 1305 AAATTC [GA]^AATT[CT]
Asp718I 2258 GGTACC G^GTACC
BbsI 120 GTCTTC GAAGAC(2/6)
BlpI 482 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 482 GCTCAGC GC^T[ACGT]AGC
BpuAI 120 GTCTTC GAAGAC(2/6)
BsaAI 193 TACGTG [CT]AC^GT[GA]
BsaBI 2222 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 9 CGACCG CG[GA][CT]^CG
BsmBI 2487 GAGACG CGTCTC(1/5)
Bsp1407I 1438 TGTACA T^GTACA
BspHI 977 TCATGA T^CATGA
BsrBI 3015 CCGCTC CCGCTC(-3/-3)
BsrDI 208 GCAATG GCAATG(2/0)
BsrFI 11 ACCGGC [GA]^CCGG[CT]
BsrGI 1438 TGTACA T^GTACA
BstEII 1097 GGTAACC G^GT[ACGT]ACC
BstPI 1097 GGTAACC G^GT[ACGT]ACC
CelII 482 GCTCAGC GC^T[ACGT]AGC
CfoI 1805 GCGC GCG^C
Cfr10I 11 ACCGGC [GA]^CCGG[CT]
DraI 1301 TTTAAA TTT^AAA
EcoO65I 1097 GGTAACC G^GT[ACGT]ACC
HaeII 1804 AGCGCC [GA]GCGC^[CT]
HhaI 1805 GCGC GCG^C
HinP1I 1805 GCGC G^CGC
KpnI 2258 GGTACC GGTAC^C
MamI 2222 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 2566 GCTAGC G^CTAGC
PaeR7I 1086 CTCGAG C^TCGAG
PflMI 2527 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2938 GACTTTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3359 AACGTT AA^CGTT
RcaI 977 TCATGA T^CATGA
SbfI 651 CCTGCAGG CCTGCA^GG
Sse8387I 651 CCTGCAGG CCTGCA^GG
SspBI 1438 TGTACA T^GTACA
SspI 3398 AATATT AAT^ATT
Van91I 2527 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1086 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 194 ACGTGT A^C[GA][CT]GT
2490 ACGTGT
AocI 802 CCTAAGG CC^T[ACGT]AGG
2369 CCTCAGG
AvaI 837 CTCGGG C^[CT]CG[GA]G
1086 CTCGAG
AvrII 1909 CCTAGG C^CTAGG
2107 CCTAGG
BamHI 1282 GGATCC G^GATCC
3007 GGATCC
BlnI 1909 CCTAGG C^CTAGG
2107 CCTAGG
BsaI 1421 GAGACC GGTCTC(1/5)
2922 GAGACC
BsmI 2272 GAATGC GAATGC(1/-1)
22 GCATTC
BsoBI 837 CTCGGG C^[CT]CG[GA]G
1086 CTCGAG
BspMI 906 GCAGGT ACCTGC(4/8)
2431 GCAGGT
BstAPI 645 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
853 GCAGCCACTGC
Bsu36I 802 CCTAAGG CC^T[ACGT]AGG
2369 CCTCAGG
Eco81I 802 CCTAAGG CC^T[ACGT]AGG
2369 CCTCAGG
FauI 2952 CCCGC CCCGC(4/6)
585 GCGGG
HgaI 90 GACGC GACGC(5/10)
3021 GCGTC
MspA1I 946 CAGCTG C[AC]G^C[GT]G
1501 CAGCTG
NspI 2065 GCATGT [GA]CATG^[CT]
2461 GCATGT
PpuMI 298 GGGACCT [GA]G^G[AT]CC[CT]
3154 AGGACCC
PstI 652 CTGCAG CTGCA^G
851 CTGCAG
PvuII 946 CAGCTG CAG^CTG
1501 CAGCTG
SacI 415 GAGCTC GAGCT^C
1823 GAGCTC
SapI 282 GAAGAGC GCTCTTC(1/4)
1431 GAAGAGC
XbaI 2046 TCTAGA T^CTAGA
2918 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI Asp700I AspHI AvaII BalI
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmnI