##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
Acc65I AccIII AgeI AhdI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BcgI BglII BlnI BsaBI
BseAI BspDI BspEI BspHI Bst1107I
BstZ17I ClaI CpoI DrdI Eam1105I
EcoRV EcoT22I FseI FspI HindIII
KpnI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI PacI PaeR7I PinAI PmeI
PshBI PvuI RcaI RsrII SalI
SbfI ScaI SgrAI SnaBI SpeI
Sse8387I SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AatII 1276 GACGTC GACGT^C
AccI 4942 GTCTAC GT^[AC][GT]AC
AflII 3986 CTTAAG C^TTAAG
AflIII 4354 ACATGT A^C[GA][CT]GT
Alw44I 1077 GTGCAC G^TGCAC
ApaLI 1077 GTGCAC G^TGCAC
BbrPI 2611 CACGTG CAC^GTG
BbvCI 4584 GCTGAGG CCTCAGC(-5/-2)
BciVI 4589 GGATAC GTATCC(6/5)
BfrI 3986 CTTAAG C^TTAAG
BsiWI 1382 CGTACG C^GTACG
Bsp1407I 3780 TGTACA T^GTACA
BspLU11I 4354 ACATGT A^CATGT
BspMI 509 GCAGGT ACCTGC(4/8)
BsrGI 3780 TGTACA T^GTACA
BssHII 200 GCGCGC G^CGCGC
BstAPI 3442 GCATCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4376 TTCGAA TT^CGAA
EcoRI 4442 GAATTC G^AATTC
HpaI 3771 GTTAAC GTT^AAC
NaeI 3358 GCCGGC GCC^GGC
NgoMIV 3358 GCCGGC G^CCGGC
NspV 4376 TTCGAA TT^CGAA
PmaCI 2611 CACGTG CAC^GTG
PmlI 2611 CACGTG CAC^GTG
PshAI 621 GACCACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 3871 ACCAGGT A^CC[AT]GGT
SfiI 323 GGCCCTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 4376 TTCGAA TT^CGAA
SphI 755 GCATGC GCATG^C
SspBI 3780 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AclI 3518 AACGTT AA^CGTT
5015 AACGTT
AcsI 3425 GAATTT [GA]^AATT[CT]
4442 GAATTC
Aor51HI 132 AGCGCT AGC^GCT
257 AGCGCT
ApoI 3425 GAATTT [GA]^AATT[CT]
4442 GAATTC
BalI 1241 TGGCCA TGG^CCA
1771 TGGCCA
BbsI 1805 GAAGAC GAAGAC(2/6)
3706 GTCTTC
BclI 992 TGATCA T^GATCA
4303 TGATCA
BpuAI 1805 GAAGAC GAAGAC(2/6)
3706 GTCTTC
BsiEI 1118 CGGCCG CG[GA][CT]^CG
1499 CGGCCG
BsmBI 3235 CGTCTC CGTCTC(1/5)
958 GAGACG
BsmI 3715 GCATTC GAATGC(1/-1)
4806 GCATTC
BsrFI 2082 ACCGGC [GA]^CCGG[CT]
3358 GCCGGC
BssSI 1688 CTCGTG CACGAG(-5/-1)
2457 CTCGTG
Cfr10I 2082 ACCGGC [GA]^CCGG[CT]
3358 GCCGGC
EagI 1118 CGGCCG C^GGCCG
1499 CGGCCG
EclXI 1118 CGGCCG C^GGCCG
1499 CGGCCG
Eco47III 132 AGCGCT AGC^GCT
257 AGCGCT
Eco52I 1118 CGGCCG C^GGCCG
1499 CGGCCG
FbaI 992 TGATCA T^GATCA
4303 TGATCA
HincII 1036 GTCAAC GT[CT]^[GA]AC
3771 GTTAAC
HindII 1036 GTCAAC GT[CT]^[GA]AC
3771 GTTAAC
MluNI 1241 TGGCCA TGG^CCA
1771 TGGCCA
MscI 1241 TGGCCA TGG^CCA
1771 TGGCCA
Psp1406I 3518 AACGTT AA^CGTT
5015 AACGTT
SmaI 374 CCCGGG CCC^GGG
1259 CCCGGG
XmaCI 374 CCCGGG C^CCGGG
1259 CCCGGG
XmaI 374 CCCGGG C^CCGGG
1259 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI Asp700I
AspHI AspI AvaI AvaII BamHI
BanI BanII BbeI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrBI BsrDI BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacI SacII SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmnI