##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AhdI Alw44I ApaLI AscI
AseI AsnI Asp718I AspEI AviII
BbeI BbrPI BbvCI BfrI BsaBI
BsaHI BsiEI BsiWI Bsp1407I BspDI
BspLU11I BsrGI BssHII BssSI BstAPI
ClaI CpoI EagI Eam1105I EclXI
Eco52I EcoRV FseI FspI Hin1I
HpaI KasI KpnI KspI MamI
MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspI PacI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SbfI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AccII 3169 CGCG CG^CG
AccIII 170 TCCGGA T^CCGGA
AgeI 2750 ACCGGT A^CCGGT
AocI 3631 CCTAAGG CC^T[ACGT]AGG
Aor51HI 575 AGCGCT AGC^GCT
AspI 1560 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4240 CCTAGG C^CTAGG
BalI 3051 TGGCCA TGG^CCA
BamHI 309 GGATCC G^GATCC
BbsI 1078 GAAGAC GAAGAC(2/6)
BcgI 2114 GCACCTCAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1165 GTATCC GTATCC(6/5)
BclI 3129 TGATCA T^GATCA
BcnI 4223 CCCGG CC^[GC]GG
BglII 175 AGATCT A^GATCT
BlnI 4240 CCTAGG C^CTAGG
BlpI 3982 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3982 GCTGAGC GC^T[ACGT]AGC
BpuAI 1078 GAAGAC GAAGAC(2/6)
BsaAI 3023 CACGTA [CT]AC^GT[GA]
BseAI 170 TCCGGA T^CCGGA
BsmI 1532 GCATTC GAATGC(1/-1)
BspEI 170 TCCGGA T^CCGGA
BspHI 1370 TCATGA T^CATGA
BsrBI 2918 CCGCTC CCGCTC(-3/-3)
BsrDI 4545 CATTGC GCAATG(2/0)
BsrFI 2750 ACCGGT [GA]^CCGG[CT]
Bst1107I 367 GTATAC GTA^TAC
BstBI 3693 TTCGAA TT^CGAA
BstUI 3169 CGCG CG^CG
BstZ17I 367 GTATAC GTA^TAC
Bsu36I 3631 CCTAAGG CC^T[ACGT]AGG
CelII 3982 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2750 ACCGGT [GA]^CCGG[CT]
DraIII 3916 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 575 AGCGCT AGC^GCT
Eco81I 3631 CCTAAGG CC^T[ACGT]AGG
EcoRI 3764 GAATTC G^AATTC
EcoT22I 842 ATGCAT ATGCA^T
FauI 3167 CCCGC CCCGC(4/6)
FbaI 3129 TGATCA T^GATCA
MluNI 3051 TGGCCA TGG^CCA
MroI 170 TCCGGA T^CCGGA
MscI 3051 TGGCCA TGG^CCA
MslI 3328 CATGGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 3169 CGCG CG^CG
NciI 4223 CCCGG CC^[GC]GG
NsiI 842 ATGCAT ATGCA^T
NspV 3693 TTCGAA TT^CGAA
PaeR7I 3656 CTCGAG C^TCGAG
PflFI 1560 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2750 ACCGGT A^CCGGT
PstI 1148 CTGCAG CTGCA^G
RcaI 1370 TCATGA T^CATGA
SacI 2314 GAGCTC GAGCT^C
SalI 2985 GTCGAC G^TCGAC
ScaI 139 AGTACT AGT^ACT
SfuI 3693 TTCGAA TT^CGAA
Tth111I 1560 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4390 TCTAGA T^CTAGA
XhoI 3656 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 367 GTATAC GT^[AC][GT]AC
2985 GTCGAC
AvaI 2309 CCCGAG C^[CT]CG[GA]G
3656 CTCGAG
BanI 2713 GGTGCC G^G[CT][GA]CC
3483 GGCACC
BsaWI 170 TCCGGA [AT]^CCGG[AT]
2750 ACCGGT
BsmBI 2686 CGTCTC CGTCTC(1/5)
3171 CGTCTC
BsoBI 2309 CCCGAG C^[CT]CG[GA]G
3656 CTCGAG
CfoI 576 GCGC GCG^C
2066 GCGC
DrdI 2342 GACCCTTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3285 GACCGTAGAGTC
EaeI 3051 TGGCCA [CT]^GGCC[GA]
4225 CGGCCA
EcoNI 1405 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1503 CCTCTCCAAGG
HaeII 575 AGCGCT [GA]GCGC^[CT]
2065 AGCGCC
HgaI 952 GACGC GACGC(5/10)
3170 GCGTC
HhaI 576 GCGC GCG^C
2066 GCGC
HinP1I 576 GCGC G^CGC
2066 GCGC
HindIII 401 AAGCTT A^AGCTT
1737 AAGCTT
MfeI 895 CAATTG C^AATTG
1802 CAATTG
MunI 895 CAATTG C^AATTG
1802 CAATTG
SapI 556 GAAGAGC GCTCTTC(1/4)
3145 GAAGAGC
SmlI 511 CTCAAG C^T[CT][GA]AG
3656 CTCGAG
XcmI 1937 CCAAGCCAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2939 CCACGTCAGGCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI Asp700I AspHI
AvaII BanII BbvI BfaI BglI
BmyI BpmI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstEII BstF5I BstNI BstPI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoO109I
EcoO65I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
PflMI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI