##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AscI AseI AsnI Asp718I BbeI
BclI BfrI BseAI BsiWI BspEI
BsrBI BssHII Bst1107I BstBI BstZ17I
CpoI DrdI EcoRI EcoRV FbaI
FseI HindIII HpaI KasI KpnI
MfeI MluI MroI MunI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PinAI PmeI PshAI
PshBI PvuI RsrII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 3872 GTCTAC GT^[AC][GT]AC
AclI 248 AACGTT AA^CGTT
AcyI 4782 GACGCC G[GA]^CG[CT]C
AhdI 6051 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4233 AGCGCT AGC^GCT
AspEI 6051 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1172 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3859 TGCGCA TGC^GCA
AvrII 7067 CCTAGG C^CTAGG
BlnI 7067 CCTAGG C^CTAGG
BsaHI 4782 GACGCC G[GA]^CG[CT]C
BsiEI 6223 CGGCCG CG[GA][CT]^CG
BsmBI 4136 GAGACG CGTCTC(1/5)
BspDI 4130 ATCGAT AT^CGAT
BspHI 873 TCATGA T^CATGA
BspLU11I 6594 ACATGT A^CATGT
BstEII 6469 GGTGACC G^GT[ACGT]ACC
BstPI 6469 GGTGACC G^GT[ACGT]ACC
ClaI 4130 ATCGAT AT^CGAT
DraI 4052 TTTAAA TTT^AAA
EagI 6223 CGGCCG C^GGCCG
Eam1105I 6051 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 6223 CGGCCG C^GGCCG
Eco47III 4233 AGCGCT AGC^GCT
Eco52I 6223 CGGCCG C^GGCCG
EcoO65I 6469 GGTGACC G^GT[ACGT]ACC
EcoT22I 4738 ATGCAT ATGCA^T
FspI 3859 TGCGCA TGC^GCA
Hin1I 4782 GACGCC G[GA]^CG[CT]C
HincII 4838 GTCAAC GT[CT]^[GA]AC
HindII 4838 GTCAAC GT[CT]^[GA]AC
KspI 6917 CCGCGG CCGC^GG
NdeI 499 CATATG CA^TATG
NsiI 4738 ATGCAT ATGCA^T
PflFI 1172 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 248 AACGTT AA^CGTT
RcaI 873 TCATGA T^CATGA
SacII 6917 CCGCGG CCGC^GG
SmaI 5643 CCCGGG CCC^GGG
SphI 4740 GCATGC GCATG^C
SspI 600 AATATT AAT^ATT
Tth111I 1172 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4987 TCTAGA T^CTAGA
XmaCI 5643 CCCGGG C^CCGGG
XmaI 5643 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
BbvCI 1323 CCTCAGC CCTCAGC(-5/-2)
47 GCTGAGG
BglI 1328 GCCCCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4202 GCCTTCATGGC
BsaBI 3011 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5222 GATGAAGATC
Bsp1407I 6116 TGTACA T^GTACA
7059 TGTACA
BsrGI 6116 TGTACA T^GTACA
7059 TGTACA
BssSI 844 CTCGTG CACGAG(-5/-1)
7001 CTCGTG
MamI 3011 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5222 GATGAAGATC
NcoI 5667 CCATGG C^CATGG
6186 CCATGG
PaeR7I 3078 CTCGAG C^TCGAG
5973 CTCGAG
SspBI 6116 TGTACA T^GTACA
7059 TGTACA
XhoI 3078 CTCGAG C^TCGAG
5973 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI ApoI Asp700I AspHI
AvaI AvaII BalI BamHI BanI
BanII BbrPI BbsI BbvI BcgI
BciVI BcnI BfaI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI