##################################
# NO CUT ENZYMES ( 43 enzymes) #
##################################
Acc65I AclI AgeI AscI Asp700I
Asp718I AvrII BbsI BciVI BlnI
BpuAI BsiWI BssHII BstBI CpoI
DraI EcoRI FseI HpaI KpnI
KspI MluI NdeI NheI NotI
NruI NspV PacI PinAI PmeI
Psp1406I PvuI RsrII SacII ScaI
SexAI SfuI SgrAI SnaBI SpeI
SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 4489 TCCGGA T^CCGGA
AcsI 3438 AAATTC [GA]^AATT[CT]
AflII 2373 CTTAAG C^TTAAG
ApoI 3438 AAATTC [GA]^AATT[CT]
AseI 5868 ATTAAT AT^TAAT
AsnI 5868 ATTAAT AT^TAAT
AviII 4566 TGCGCA TGC^GCA
BamHI 3427 GGATCC G^GATCC
BcgI 1259 CGATGACTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2373 CTTAAG C^TTAAG
BsaBI 5972 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 4489 TCCGGA T^CCGGA
Bsp1407I 1480 TGTACA T^GTACA
BspEI 4489 TCCGGA T^CCGGA
BsrGI 1480 TGTACA T^GTACA
BstAPI 2242 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EcoRV 4104 GATATC GAT^ATC
EcoT22I 4962 ATGCAT ATGCA^T
FspI 4566 TGCGCA TGC^GCA
HindIII 4081 AAGCTT A^AGCTT
MamI 5972 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4526 CAATTG C^AATTG
MroI 4489 TCCGGA T^CCGGA
MunI 4526 CAATTG C^AATTG
NsiI 4962 ATGCAT ATGCA^T
PaeR7I 4000 CTCGAG C^TCGAG
PshAI 110 GACCGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5868 ATTAAT AT^TAAT
SalI 3279 GTCGAC G^TCGAC
SapI 752 GCTCTTC GCTCTTC(1/4)
SfiI 4386 GGCCTGCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 310 GCATGC GCATG^C
SspBI 1480 TGTACA T^GTACA
SspI 2295 AATATT AAT^ATT
XcmI 1720 CCAGGGTCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4000 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AhdI 477 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
825 GACCCTGAGTC
AocI 996 CCTGAGG CC^T[ACGT]AGG
2589 CCTCAGG
Aor51HI 1684 AGCGCT AGC^GCT
5328 AGCGCT
AspEI 477 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
825 GACCCTGAGTC
BbrPI 3852 CACGTG CAC^GTG
4248 CACGTG
BclI 1679 TGATCA T^GATCA
5128 TGATCA
BglI 2407 GCCAGCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4387 GCCTGCAGGGC
BsaWI 2662 ACCGGA [AT]^CCGG[AT]
4489 TCCGGA
BsmBI 12 CGTCTC CGTCTC(1/5)
183 CGTCTC
BsmI 5696 GAATGC GAATGC(1/-1)
5908 GCATTC
BspLU11I 1813 ACATGT A^CATGT
5353 ACATGT
BsrDI 3362 CATTGC GCAATG(2/0)
3980 CATTGC
BssSI 6165 CACGAG CACGAG(-5/-1)
2973 CTCGTG
Bst1107I 1748 GTATAC GTA^TAC
5087 GTATAC
BstZ17I 1748 GTATAC GTA^TAC
5087 GTATAC
Bsu36I 996 CCTGAGG CC^T[ACGT]AGG
2589 CCTCAGG
DraIII 2883 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
5727 CACGCCGTG
Eam1105I 477 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
825 GACCCTGAGTC
Eco47III 1684 AGCGCT AGC^GCT
5328 AGCGCT
Eco81I 996 CCTGAGG CC^T[ACGT]AGG
2589 CCTCAGG
FbaI 1679 TGATCA T^GATCA
5128 TGATCA
NcoI 1601 CCATGG C^CATGG
2843 CCATGG
PflMI 1439 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2734 CCAGCCCCTGG
PmaCI 3852 CACGTG CAC^GTG
4248 CACGTG
PmlI 3852 CACGTG CAC^GTG
4248 CACGTG
SbfI 1136 CCTGCAGG CCTGCA^GG
4388 CCTGCAGG
Sse8387I 1136 CCTGCAGG CCTGCA^GG
4388 CCTGCAGG
StuI 1180 AGGCCT AGG^CCT
1843 AGGCCT
Van91I 1439 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2734 CCAGCCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 150 enzymes) #
#########################################
AatII AccI AccII AciI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI ApaI ApaLI AspHI AspI
AvaI AvaII BalI BanI BanII
BbeI BbvCI BbvI BcnI BfaI
BglII BlpI BmyI BpmI Bpu1102I
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspDI
BspHI BspMI BsrBI BsrFI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I ClaI DdeI
DpnII DraII DrdI DsaI EaeI
EagI EarI EclXI Eco52I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflFI PleI PpuMI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XhoII XmaCI XmaI