##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AflIII AgeI AhdI Alw44I
ApaLI AscI AseI AsnI AspEI
AvrII BbeI BlnI BsaBI BsiWI
BspDI BspLU11I BssSI BstBI ClaI
CpoI EagI Eam1105I EclXI Eco52I
EcoRV FseI HpaI KasI MamI
MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
PvuI RsrII SalI SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SpeI StuI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 3378 GGTACC G^GTACC
AccIII 862 TCCGGA T^CCGGA
AclI 4201 AACGTT AA^CGTT
Aor51HI 5898 AGCGCT AGC^GCT
Asp718I 3378 GGTACC G^GTACC
AspI 1166 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 6220 TGCGCA TGC^GCA
BbrPI 2447 CACGTG CAC^GTG
BcgI 5439 CGAGCAGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3660 GTATCC GTATCC(6/5)
BclI 5043 TGATCA T^GATCA
BsaWI 862 TCCGGA [AT]^CCGG[AT]
BseAI 862 TCCGGA T^CCGGA
BsiEI 1030 CGACCG CG[GA][CT]^CG
Bsp1407I 4346 TGTACA T^GTACA
BspEI 862 TCCGGA T^CCGGA
BspHI 5754 TCATGA T^CATGA
BsrGI 4346 TGTACA T^GTACA
BssHII 3882 GCGCGC G^CGCGC
Bst1107I 5629 GTATAC GTA^TAC
BstAPI 4511 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 5629 GTATAC GTA^TAC
Eco47III 5898 AGCGCT AGC^GCT
FbaI 5043 TGATCA T^GATCA
FspI 6220 TGCGCA TGC^GCA
HindIII 2282 AAGCTT A^AGCTT
KpnI 3378 GGTACC GGTAC^C
KspI 364 CCGCGG CCGC^GG
MroI 862 TCCGGA T^CCGGA
PflFI 1166 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2447 CACGTG CAC^GTG
PmlI 2447 CACGTG CAC^GTG
Psp1406I 4201 AACGTT AA^CGTT
RcaI 5754 TCATGA T^CATGA
SacI 590 GAGCTC GAGCT^C
SacII 364 CCGCGG CCGC^GG
SbfI 2956 CCTGCAGG CCTGCA^GG
SnaBI 4089 TACGTA TAC^GTA
SphI 5310 GCATGC GCATG^C
Sse8387I 2956 CCTGCAGG CCTGCA^GG
SspBI 4346 TGTACA T^GTACA
SspI 5242 AATATT AAT^ATT
Tth111I 1166 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3424 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 1547 GGCGTC G[GA]^CG[CT]C
2717 GACGCC
AflII 1145 CTTAAG C^TTAAG
4226 CTTAAG
AocI 2548 CCTGAGG CC^T[ACGT]AGG
3076 CCTGAGG
ApaI 3208 GGGCCC GGGCC^C
4709 GGGCCC
AvaI 435 CCCGGG C^[CT]CG[GA]G
1896 CCCGGG
BamHI 852 GGATCC G^GATCC
859 GGATCC
BfrI 1145 CTTAAG C^TTAAG
4226 CTTAAG
BglI 2877 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3228 GCCCAGAGGGC
BsaAI 2447 CACGTG [CT]AC^GT[GA]
4089 TACGTA
BsaHI 1547 GGCGTC G[GA]^CG[CT]C
2717 GACGCC
BsmBI 2804 CGTCTC CGTCTC(1/5)
6251 GAGACG
BsoBI 435 CCCGGG C^[CT]CG[GA]G
1896 CCCGGG
Bsu36I 2548 CCTGAGG CC^T[ACGT]AGG
3076 CCTGAGG
DraI 4034 TTTAAA TTT^AAA
6506 TTTAAA
DrdI 1901 GACGGAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2966 GACACAGTGGTC
Eco81I 2548 CCTGAGG CC^T[ACGT]AGG
3076 CCTGAGG
EcoT22I 1003 ATGCAT ATGCA^T
6468 ATGCAT
Hin1I 1547 GGCGTC G[GA]^CG[CT]C
2717 GACGCC
HincII 914 GTCAAC GT[CT]^[GA]AC
3754 GTTGAC
HindII 914 GTCAAC GT[CT]^[GA]AC
3754 GTTGAC
MfeI 5234 CAATTG C^AATTG
6001 CAATTG
MunI 5234 CAATTG C^AATTG
6001 CAATTG
NsiI 1003 ATGCAT ATGCA^T
6468 ATGCAT
SmaI 435 CCCGGG CCC^GGG
1896 CCCGGG
TaqI 1956 TCGA T^CGA
2153 TCGA
TthHB8I 1956 TCGA T^CGA
2153 TCGA
XcmI 856 CCAGGATCCGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4732 CCAAAGCAGCCATGG
XmaCI 435 CCCGGG C^CCGGG
1896 CCCGGG
XmaI 435 CCCGGG C^CCGGG
1896 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoNI EcoO109I EcoO65I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII
XmnI