##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BamHI BbeI
BciVI BfrI BlnI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrGI
BssHII Bst1107I BstEII BstPI BstZ17I
ClaI CpoI DraI DraIII EagI
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FseI FspI HincII HindII
HpaI KasI KpnI KspI MfeI
MluI MunI NarI NdeI NheI
NotI NruI NsiI PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfoI SgrAI SmaI
SnaBI Sse8387I SspBI SspI SwaI
XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 790 GACGTC GACGT^C
AccIII 1607 TCCGGA T^CCGGA
AflIII 1103 ACGTGT A^C[GA][CT]GT
AhdI 2628 GACTGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 400 GTGCAC G^TGCAC
AlwNI 3275 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 400 GTGCAC G^TGCAC
AspEI 2628 GACTGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2224 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 2434 CGAGACCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1409 TGATCA T^GATCA
BglII 291 AGATCT A^GATCT
BsaBI 2295 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1607 TCCGGA T^CCGGA
BsiEI 3247 CGACCG CG[GA][CT]^CG
BspEI 1607 TCCGGA T^CCGGA
BstBI 114 TTCGAA TT^CGAA
DrdI 856 GACGAGCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2628 GACTGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 117 GAATTC G^AATTC
FbaI 1409 TGATCA T^GATCA
HindIII 904 AAGCTT A^AGCTT
MamI 2295 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1607 TCCGGA T^CCGGA
MslI 5 CACCAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspV 114 TTCGAA TT^CGAA
PaeR7I 544 CTCGAG C^TCGAG
PflFI 2224 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 2001 GAGCTC GAGCT^C
SapI 1827 GAAGAGC GCTCTTC(1/4)
SfuI 114 TTCGAA TT^CGAA
SpeI 2239 ACTAGT A^CTAGT
SphI 1245 GCATGC GCATG^C
Tth111I 2224 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 544 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AocI 220 CCTCAGG CC^T[ACGT]AGG
2171 CCTCAGG
BalI 984 TGGCCA TGG^CCA
1712 TGGCCA
BbrPI 1102 CACGTG CAC^GTG
2211 CACGTG
BfaI 229 CTAG C^TAG
2240 CTAG
BlpI 1641 GCTGAGC GC^T[ACGT]AGC
2739 GCTTAGC
Bpu1102I 1641 GCTGAGC GC^T[ACGT]AGC
2739 GCTTAGC
BsaAI 1102 CACGTG [CT]AC^GT[GA]
2211 CACGTG
BsaWI 572 ACCGGA [AT]^CCGG[AT]
1607 TCCGGA
BsgI 1261 GTGCAG GTGCAG(16/14)
1906 GTGCAG
BspMI 680 ACCTGC ACCTGC(4/8)
2930 ACCTGC
BsrDI 126 CATTGC GCAATG(2/0)
1733 CATTGC
BsrFI 2263 GCCGGC [GA]^CCGG[CT]
3029 GCCGGC
BstAPI 2322 GCAGCACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2604 GCAGGGATTGC
BstYI 291 AGATCT [GA]^GATC[CT]
2300 AGATCC
Bsu36I 220 CCTCAGG CC^T[ACGT]AGG
2171 CCTCAGG
CelII 1641 GCTGAGC GC^T[ACGT]AGC
2739 GCTTAGC
Cfr10I 2263 GCCGGC [GA]^CCGG[CT]
3029 GCCGGC
Eco81I 220 CCTCAGG CC^T[ACGT]AGG
2171 CCTCAGG
EcoNI 1293 CCTCTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1710 CCTGGCCAAGG
HaeII 1876 AGCGCC [GA]GCGC^[CT]
2061 GGCGCT
MaeI 229 CTAG C^TAG
2240 CTAG
MflI 291 AGATCT [GA]^GATC[CT]
2300 AGATCC
MluNI 984 TGGCCA TGG^CCA
1712 TGGCCA
MscI 984 TGGCCA TGG^CCA
1712 TGGCCA
NaeI 2263 GCCGGC GCC^GGC
3029 GCCGGC
NgoMIV 2263 GCCGGC G^CCGGC
3029 GCCGGC
PflMI 1620 CCAGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2460 CCAGCCCGTGG
PmaCI 1102 CACGTG CAC^GTG
2211 CACGTG
PmlI 1102 CACGTG CAC^GTG
2211 CACGTG
SfiI 1279 GGCCCTGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3194 GGCCCTCCAGGCC
TfiI 1060 GAATC G^A[AT]TC
1763 GATTC
Van91I 1620 CCAGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2460 CCAGCCCGTGG
XhoII 291 AGATCT [GA]^GATC[CT]
2300 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApaI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BcnI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BsrBI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI