##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AhdI AscI AseI AsnI Asp700I
Asp718I AspEI BamHI BciVI BclI
BfrI BglII BsaBI BsiWI Bsp1407I
BspDI BspLU11I BsrGI BssSI Bst1107I
BstBI BstZ17I ClaI CpoI DraIII
DrdI Eam1105I EcoRI EcoRV FbaI
HincII HindII HindIII HpaI KpnI
MamI MfeI MluI MunI NheI
NotI NspV PacI PaeR7I PflMI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SalI ScaI SfiI SfuI
SmlI SnaBI SpeI SphI SspBI
SspI SwaI Van91I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 955 GACGTC GACGT^C
AccIII 1033 TCCGGA T^CCGGA
Alw44I 3961 GTGCAC G^TGCAC
ApaLI 3961 GTGCAC G^TGCAC
AspI 1397 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3155 CCTAGG C^CTAGG
BbvCI 1373 GCTGAGG CCTCAGC(-5/-2)
BcgI 1573 GCAGGAGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 3155 CCTAGG C^CTAGG
BseAI 1033 TCCGGA T^CCGGA
BspEI 1033 TCCGGA T^CCGGA
BspHI 2667 TCATGA T^CATGA
BsrDI 371 GCAATG GCAATG(2/0)
BstEII 2500 GGTCACC G^GT[ACGT]ACC
BstPI 2500 GGTCACC G^GT[ACGT]ACC
DraI 856 TTTAAA TTT^AAA
EcoO65I 2500 GGTCACC G^GT[ACGT]ACC
EcoT22I 1040 ATGCAT ATGCA^T
FseI 267 GGCCGGCC GGCCGG^CC
MroI 1033 TCCGGA T^CCGGA
NdeI 574 CATATG CA^TATG
NruI 1582 TCGCGA TCG^CGA
NsiI 1040 ATGCAT ATGCA^T
NspI 1791 ACATGC [GA]CATG^[CT]
PflFI 1397 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2494 GACCCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2667 TCATGA T^CATGA
SacI 3331 GAGCTC GAGCT^C
SbfI 2317 CCTGCAGG CCTGCA^GG
SexAI 1416 ACCTGGT A^CC[AT]GGT
Sse8387I 2317 CCTGCAGG CCTGCA^GG
Tth111I 1397 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 3658 CCATTTAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 501 GAATTT [GA]^AATT[CT]
3991 AAATTT
AflIII 1359 ACGTGT A^C[GA][CT]GT
2766 ACGTGT
Aor51HI 1026 AGCGCT AGC^GCT
2328 AGCGCT
ApoI 501 GAATTT [GA]^AATT[CT]
3991 AAATTT
AviII 1248 TGCGCA TGC^GCA
2682 TGCGCA
BbrPI 2765 CACGTG CAC^GTG
3751 CACGTG
BlpI 2109 GCTCAGC GC^T[ACGT]AGC
3333 GCTCAGC
Bpu1102I 2109 GCTCAGC GC^T[ACGT]AGC
3333 GCTCAGC
BsaAI 2765 CACGTG [CT]AC^GT[GA]
3751 CACGTG
BsaWI 1033 TCCGGA [AT]^CCGG[AT]
1492 ACCGGA
BsmI 2560 GAATGC GAATGC(1/-1)
2975 GAATGC
CelII 2109 GCTCAGC GC^T[ACGT]AGC
3333 GCTCAGC
Eco47III 1026 AGCGCT AGC^GCT
2328 AGCGCT
FspI 1248 TGCGCA TGC^GCA
2682 TGCGCA
PmaCI 2765 CACGTG CAC^GTG
3751 CACGTG
PmlI 2765 CACGTG CAC^GTG
3751 CACGTG
SapI 2216 GAAGAGC GCTCTTC(1/4)
1311 GCTCTTC
SgrAI 805 CGCCGGCG C[GA]^CCGG[CT]G
1727 CGCCGGCG
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssHII BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmaCI
XmaI