##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AccI AccII AccIII AclI AgeI
AhdI Alw44I ApaI ApaLI AscI
AspEI AvrII BalI BbeI BbrPI
BcgI BglI BglII BlnI BlpI
Bpu1102I BsaI BseAI BsiEI BsiWI
BsmI BspDI BspEI BsrFI BssHII
BssSI Bst1107I BstBI BstUI BstZ17I
CelII Cfr10I ClaI CpoI DrdI
EaeI EagI Eam1105I EclXI Eco52I
EcoRI EcoRV FseI KasI KspI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI Sse8387I Van91I
XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 3548 GACGTC GACGT^C
Aor51HI 3598 AGCGCT AGC^GCT
AseI 4045 ATTAAT AT^TAAT
AsnI 4045 ATTAAT AT^TAAT
AspHI 369 GAGCTC G[AT]GC[AT]^C
AspI 77 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2823 CCCGGG C^[CT]CG[GA]G
AviII 3962 TGCGCA TGC^GCA
BbvCI 19 CCTCAGC CCTCAGC(-5/-2)
BsaWI 2848 TCCGGT [AT]^CCGG[AT]
BsiHKAI 369 GAGCTC G[AT]GC[AT]^C
BsoBI 2823 CCCGGG C^[CT]CG[GA]G
BspMI 592 GCAGGT ACCTGC(4/8)
BsrBI 1651 GAGCGG CCGCTC(-3/-3)
BstEII 574 GGTTACC G^GT[ACGT]ACC
BstPI 574 GGTTACC G^GT[ACGT]ACC
DraIII 1888 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 846 CCACGG C^C[GA][CT]GG
Eco47III 3598 AGCGCT AGC^GCT
EcoNI 4249 CCTGAGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 574 GGTTACC G^GT[ACGT]ACC
FauI 1938 GCGGG CCCGC(4/6)
FspI 3962 TGCGCA TGC^GCA
HaeII 3598 AGCGCT [GA]GCGC^[CT]
HgaI 1656 GCGTC GACGC(5/10)
NdeI 3387 CATATG CA^TATG
PflFI 77 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 3565 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1359 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4045 ATTAAT AT^TAAT
PstI 519 CTGCAG CTGCA^G
SacI 369 GAGCTC GAGCT^C
SapI 541 GCTCTTC GCTCTTC(1/4)
SmaI 2823 CCCGGG CCC^GGG
StuI 2808 AGGCCT AGG^CCT
SwaI 3456 ATTTAAAT ATTT^AAAT
Tth111I 77 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2641 CCAAGTGTTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2823 CCCGGG C^CCGGG
XmaI 2823 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 1655 GGCGTC G[GA]^CG[CT]C
3548 GACGTC
AocI 798 CCTCAGG CC^T[ACGT]AGG
2401 CCTAAGG
Asp700I 189 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
377 GAAGTACTTC
BamHI 3675 GGATCC G^GATCC
4351 GGATCC
BciVI 967 GGATAC GTATCC(6/5)
2930 GGATAC
BsaHI 1655 GGCGTC G[GA]^CG[CT]C
3548 GACGTC
BsmBI 3550 CGTCTC CGTCTC(1/5)
3546 GAGACG
Bsp1407I 2191 TGTACA T^GTACA
3762 TGTACA
BsrGI 2191 TGTACA T^GTACA
3762 TGTACA
BstAPI 1571 GCATGCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2136 GCATGTCTTGC
BstXI 3555 CCATGTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3862 CCACTGATTTGG
Bsu36I 798 CCTCAGG CC^T[ACGT]AGG
2401 CCTAAGG
CfoI 3599 GCGC GCG^C
3963 GCGC
DraII 2826 GGGGCCT [GA]G^G[ACGT]CC[CT]
3565 GGGTCCT
EarI 542 CTCTTC CTCTTC(1/4)
4929 GAAGAG
Eco81I 798 CCTCAGG CC^T[ACGT]AGG
2401 CCTAAGG
EcoO109I 2826 GGGGCCT [GA]G^G[ACGT]CC[CT]
3565 GGGTCCT
HhaI 3599 GCGC GCG^C
3963 GCGC
Hin1I 1655 GGCGTC G[GA]^CG[CT]C
3548 GACGTC
HinP1I 3599 GCGC G^CGC
3963 GCGC
HpaI 1146 GTTAAC GTT^AAC
3043 GTTAAC
Ksp632I 542 CTCTTC CTCTTC(1/4)
4929 GAAGAG
MspA1I 1509 CAGCTG C[AC]G^C[GT]G
4070 CAGCTG
PvuII 1509 CAGCTG CAG^CTG
4070 CAGCTG
SphI 1571 GCATGC GCATG^C
3630 GCATGC
SspBI 2191 TGTACA T^GTACA
3762 TGTACA
TaqI 9 TCGA T^CGA
1931 TCGA
TthHB8I 9 TCGA T^CGA
1931 TCGA
XbaI 1706 TCTAGA T^CTAGA
3007 TCTAGA
XmnI 189 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
377 GAAGTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
Acc65I AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI ApoI
Asp718I AvaII BanI BanII BbsI
BbvI BclI BcnI BfaI BfrI
BmyI BpmI BpuAI BsaAI BsaBI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BspLU11I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EcoRII EcoT14I EcoT22I FbaI
Fnu4HI FokI HaeIII HapII HincII
HindII HindIII HinfI HpaII HphI
ItaI KpnI MaeI MaeII MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PleI RcaI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SnaBI SpeI SspI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII