##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AgeI Aor51HI ApoI AscI AseI
AsnI Asp718I AspI BciVI BclI
BfrI BglII BsiWI BspDI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI EagI EclXI Eco47III Eco52I
EcoRI FbaI HincII HindII HindIII
HpaI KpnI KspI MluI NdeI
NheI NotI NruI NspV PacI
PflFI PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI ScaI
SfuI SgrAI SnaBI SpeI SspI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 778 GACGTC GACGT^C
AccII 5911 CGCG CG^CG
AhdI 293 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 5391 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 293 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 5489 TGCGCA TGC^GCA
AvrII 691 CCTAGG C^CTAGG
BamHI 1332 GGATCC G^GATCC
BlnI 691 CCTAGG C^CTAGG
BlpI 4892 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4892 GCTCAGC GC^T[ACGT]AGC
BsaBI 4820 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 5073 CGGTCG CG[GA][CT]^CG
BsmBI 4447 CGTCTC CGTCTC(1/5)
Bsp1407I 4870 TGTACA T^GTACA
BsrGI 4870 TGTACA T^GTACA
BstUI 5911 CGCG CG^CG
CelII 4892 GCTCAGC GC^T[ACGT]AGC
DraIII 3665 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 5066 GACACCTCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 293 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1185 GATATC GAT^ATC
EcoT22I 2753 ATGCAT ATGCA^T
FspI 5489 TGCGCA TGC^GCA
MamI 4820 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4556 CAATTG C^AATTG
MunI 4556 CAATTG C^AATTG
MvnI 5911 CGCG CG^CG
NsiI 2753 ATGCAT ATGCA^T
PaeR7I 3678 CTCGAG C^TCGAG
PshAI 2 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 3431 GAGCTC GAGCT^C
SapI 5316 GAAGAGC GCTCTTC(1/4)
SbfI 5344 CCTGCAGG CCTGCA^GG
SexAI 6052 ACCTGGT A^CC[AT]GGT
Sse8387I 5344 CCTGCAGG CCTGCA^GG
SspBI 4870 TGTACA T^GTACA
XhoI 3678 CTCGAG C^TCGAG
XmnI 5391 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccIII 1634 TCCGGA T^CCGGA
6027 TCCGGA
BbeI 1469 GGCGCC GGCGC^C
4925 GGCGCC
BcgI 44 CGAGGGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5689 GCAGGGTGGTCG
BsaWI 1634 TCCGGA [AT]^CCGG[AT]
6027 TCCGGA
BseAI 1634 TCCGGA T^CCGGA
6027 TCCGGA
BspEI 1634 TCCGGA T^CCGGA
6027 TCCGGA
BspLU11I 772 ACATGT A^CATGT
3007 ACATGT
BsrBI 4058 CCGCTC CCGCTC(-3/-3)
4664 CCGCTC
BsrDI 1076 GCAATG GCAATG(2/0)
1851 GCAATG
FseI 2007 GGCCGGCC GGCCGG^CC
3186 GGCCGGCC
KasI 1469 GGCGCC G^GCGCC
4925 GGCGCC
MroI 1634 TCCGGA T^CCGGA
6027 TCCGGA
NarI 1469 GGCGCC GG^CGCC
4925 GGCGCC
SfiI 699 GGCCCAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
5760 GGCCAGAGAGGCC
SfoI 1469 GGCGCC GGC^GCC
4925 GGCGCC
SmaI 1423 CCCGGG CCC^GGG
2688 CCCGGG
SphI 4029 GCATGC GCATG^C
4943 GCATGC
XcmI 165 CCATAGAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3334 CCAGTGGCTTTCTGG
XmaCI 1423 CCCGGG C^CCGGG
2688 CCCGGG
XmaI 1423 CCCGGG C^CCGGG
2688 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbrPI BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspHI BspMI BsrFI BsrI
BssKI BssSI BstAPI BstEII BstF5I
BstNI BstPI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII