##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AscI AseI AsnI Asp700I
Asp718I AspI AvrII BamHI BfrI
BlnI BseAI BsiEI BsiWI BsmI
BspDI BspEI BsrBI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DraI
EagI EclXI Eco52I EcoNI EcoRI
EcoRV EcoT22I FseI HpaI KpnI
KspI MfeI MluI MroI MunI
NdeI NotI NruI NsiI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SfiI
SfuI SgrAI SnaBI SpeI SphI
SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 673 GTCGAC GT^[AC][GT]AC
AhdI 665 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3072 CCTGAGG CC^T[ACGT]AGG
ApaI 3078 GGGCCC GGGCC^C
AspEI 665 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2487 TGCGCA TGC^GCA
BbeI 1721 GGCGCC GGCGC^C
BbvCI 2922 CCTCAGC CCTCAGC(-5/-2)
BcgI 403 CGAGCTCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2440 GGATAC GTATCC(6/5)
BglI 888 GCCTCAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3403 AGATCT A^GATCT
BsmBI 3256 GAGACG CGTCTC(1/5)
Bsp1407I 297 TGTACA T^GTACA
BspMI 1675 GCAGGT ACCTGC(4/8)
BsrFI 707 GCCGGC [GA]^CCGG[CT]
BsrGI 297 TGTACA T^GTACA
BstAPI 28 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2041 GGTCACC G^GT[ACGT]ACC
BstPI 2041 GGTCACC G^GT[ACGT]ACC
Bsu36I 3072 CCTGAGG CC^T[ACGT]AGG
Cfr10I 707 GCCGGC [GA]^CCGG[CT]
DrdI 512 GACCACGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 665 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3072 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2041 GGTCACC G^GT[ACGT]ACC
FspI 2487 TGCGCA TGC^GCA
KasI 1721 GGCGCC G^GCGCC
NaeI 707 GCCGGC GCC^GGC
NarI 1721 GGCGCC GG^CGCC
NgoMIV 707 GCCGGC G^CCGGC
NheI 2740 GCTAGC G^CTAGC
PaeR7I 2809 CTCGAG C^TCGAG
PshAI 676 GACTGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 673 GTCGAC G^TCGAC
SapI 173 GAAGAGC GCTCTTC(1/4)
SbfI 1072 CCTGCAGG CCTGCA^GG
SexAI 2781 ACCAGGT A^CC[AT]GGT
SfoI 1721 GGCGCC GGC^GCC
SmaI 1419 CCCGGG CCC^GGG
Sse8387I 1072 CCTGCAGG CCTGCA^GG
SspBI 297 TGTACA T^GTACA
SspI 3342 AATATT AAT^ATT
XhoI 2809 CTCGAG C^TCGAG
XmaCI 1419 CCCGGG C^CCGGG
XmaI 1419 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccII 725 CGCG CG^CG
2425 CGCG
AcsI 2841 GAATTT [GA]^AATT[CT]
3506 GAATTT
AlwI 43 GATCC GGATC(4/5)
460 GATCC
Aor51HI 651 AGCGCT AGC^GCT
2391 AGCGCT
ApoI 2841 GAATTT [GA]^AATT[CT]
3506 GAATTT
BbrPI 224 CACGTG CAC^GTG
2063 CACGTG
BclI 2277 TGATCA T^GATCA
3281 TGATCA
BfaI 812 CTAG C^TAG
2741 CTAG
BlpI 1540 GCTCAGC GC^T[ACGT]AGC
1918 GCTCAGC
Bpu1102I 1540 GCTCAGC GC^T[ACGT]AGC
1918 GCTCAGC
BsaBI 454 GATTAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1318 GATACTCATC
BsaI 956 GAGACC GGTCTC(1/5)
3231 GGTCTC
BsaWI 1464 ACCGGA [AT]^CCGG[AT]
2830 ACCGGA
BsgI 1300 CTGCAC GTGCAG(16/14)
1673 GTGCAG
BspHI 1911 TCATGA T^CATGA
3323 TCATGA
BspLU11I 300 ACATGT A^CATGT
2049 ACATGT
BsrDI 844 CATTGC GCAATG(2/0)
1572 GCAATG
BssSI 311 CACGAG CACGAG(-5/-1)
1118 CACGAG
BstUI 725 CGCG CG^CG
2425 CGCG
CelII 1540 GCTCAGC GC^T[ACGT]AGC
1918 GCTCAGC
DraIII 1685 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
2780 CACCAGGTG
Eco47III 651 AGCGCT AGC^GCT
2391 AGCGCT
FbaI 2277 TGATCA T^GATCA
3281 TGATCA
HincII 673 GTCGAC GT[CT]^[GA]AC
2339 GTCAAC
HindII 673 GTCGAC GT[CT]^[GA]AC
2339 GTCAAC
HindIII 1436 AAGCTT A^AGCTT
3547 AAGCTT
MaeI 812 CTAG C^TAG
2741 CTAG
MamI 454 GATTAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1318 GATACTCATC
MvnI 725 CGCG CG^CG
2425 CGCG
NcoI 546 CCATGG C^CATGG
747 CCATGG
PmaCI 224 CACGTG CAC^GTG
2063 CACGTG
PmlI 224 CACGTG CAC^GTG
2063 CACGTG
PpuMI 1077 AGGACCT [GA]G^G[AT]CC[CT]
1564 GGGACCC
PstI 370 CTGCAG CTGCA^G
1073 CTGCAG
RcaI 1911 TCATGA T^CATGA
3323 TCATGA
ScaI 1554 AGTACT AGT^ACT
1884 AGTACT
StuI 126 AGGCCT AGG^CCT
2034 AGGCCT
TfiI 1372 GATTC G^A[AT]TC
1833 GATTC
Tsp509I 2842 AATT ^AATT
3507 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
Alw44I AlwNI ApaLI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI Tru9I TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoII