##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccIII AcyI AgeI AocI
Aor51HI ApaI AscI AspI BbeI
BcgI BglII BlpI Bpu1102I BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BspEI BssHII BssSI
BstBI BstEII BstPI Bsu36I CelII
CpoI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I FseI HaeII
Hin1I KasI MamI MfeI MluI
MroI MunI NarI NheI NotI
NruI NspV PacI PflFI PinAI
PmeI PvuI RsrII SalI SbfI
SfoI SfuI SgrAI SphI Sse8387I
Tth111I
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AclI 4524 AACGTT AA^CGTT
AhdI 5379 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 566 GTGCAC G^TGCAC
ApaLI 566 GTGCAC G^TGCAC
AspEI 5379 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 796 TGCGCA TGC^GCA
BamHI 2221 GGATCC G^GATCC
BanII 774 GAGCTC G[GA]GC[CT]^C
BbrPI 3229 CACGTG CAC^GTG
BbvCI 438 GCTGAGG CCTCAGC(-5/-2)
BclI 5433 TGATCA T^GATCA
BglI 2290 GCCCCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 945 GAGACC GGTCTC(1/5)
BspDI 2365 ATCGAT AT^CGAT
BsrBI 1472 GAGCGG CCGCTC(-3/-3)
Bst1107I 3502 GTATAC GTA^TAC
BstAPI 214 GCAGTATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3502 GTATAC GTA^TAC
ClaI 2365 ATCGAT AT^CGAT
DraIII 4874 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4004 GACTTTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 5379 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 4777 GATATC GAT^ATC
EcoT22I 3860 ATGCAT ATGCA^T
FbaI 5433 TGATCA T^GATCA
FspI 796 TGCGCA TGC^GCA
KspI 1023 CCGCGG CCGC^GG
NaeI 120 GCCGGC GCC^GGC
NgoMIV 120 GCCGGC G^CCGGC
NsiI 3860 ATGCAT ATGCA^T
PaeR7I 4907 CTCGAG C^TCGAG
PmaCI 3229 CACGTG CAC^GTG
PmlI 3229 CACGTG CAC^GTG
Psp1406I 4524 AACGTT AA^CGTT
SacI 774 GAGCTC GAGCT^C
SacII 1023 CCGCGG CCGC^GG
SapI 690 GCTCTTC GCTCTTC(1/4)
SexAI 1974 ACCAGGT A^CC[AT]GGT
SfiI 2289 GGCCCCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1466 CCCGGG CCC^GGG
SnaBI 6591 TACGTA TAC^GTA
SpeI 4671 ACTAGT A^CTAGT
XcmI 2225 CCAGGTTTTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4907 CTCGAG C^TCGAG
XmaCI 1466 CCCGGG C^CCGGG
XmaI 1466 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 1575 GGTACC G^GTACC
4410 GGTACC
AflII 2632 CTTAAG C^TTAAG
2968 CTTAAG
Asp718I 1575 GGTACC G^GTACC
4410 GGTACC
AvrII 2801 CCTAGG C^CTAGG
3311 CCTAGG
BfrI 2632 CTTAAG C^TTAAG
2968 CTTAAG
BlnI 2801 CCTAGG C^CTAGG
3311 CCTAGG
BspHI 5201 TCATGA T^CATGA
6078 TCATGA
BspLU11I 3289 ACATGT A^CATGT
4818 ACATGT
EcoRI 2106 GAATTC G^AATTC
6310 GAATTC
HgaI 1726 GACGC GACGC(5/10)
5293 GACGC
KpnI 1575 GGTACC GGTAC^C
4410 GGTACC
NcoI 935 CCATGG C^CATGG
2406 CCATGG
PflMI 2280 CCACTCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2294 CCAAGGCCTGG
PshAI 947 GACCGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4549 GACTCTTGTC
RcaI 5201 TCATGA T^CATGA
6078 TCATGA
ScaI 3072 AGTACT AGT^ACT
4417 AGTACT
StuI 631 AGGCCT AGG^CCT
2297 AGGCCT
SwaI 3848 ATTTAAAT ATTT^AAAT
5063 ATTTAAAT
Van91I 2280 CCACTCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2294 CCAAGGCCTGG
XbaI 4557 TCTAGA T^CTAGA
6162 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
AseI AsnI Asp700I AspHI AvaI
AvaII BalI BanI BbsI BbvI
BciVI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrDI
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeI NdeII NlaIII NlaIV
NspI PleI PpuMI PshBI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI