##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII AgeI AscI AseI AsnI
AviII BcgI BciVI BclI BglII
BsaBI BspDI BssHII Bst1107I BstZ17I
ClaI CpoI FbaI FseI FspI
HpaI KspI MamI MfeI MluI
MunI NdeI NotI NruI PacI
PaeR7I PflMI PinAI PmeI PshAI
PshBI PvuI RsrII SacII SalI
SbfI SexAI SfiI SgrAI SnaBI
Sse8387I SspI StuI SwaI Van91I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 321 GGTACC G^GTACC
AccI 1201 GTCTAC GT^[AC][GT]AC
AccIII 6017 TCCGGA T^CCGGA
AclI 1288 AACGTT AA^CGTT
AhdI 5788 GACCCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4359 GTGCAC G^TGCAC
ApaI 5514 GGGCCC GGGCC^C
ApaLI 4359 GTGCAC G^TGCAC
Asp700I 4973 GAATACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 321 GGTACC G^GTACC
AspEI 5788 GACCCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1245 GGATCC G^GATCC
BseAI 6017 TCCGGA T^CCGGA
BsiEI 5114 CGGCCG CG[GA][CT]^CG
BsiWI 3657 CGTACG C^GTACG
BsmBI 2350 GAGACG CGTCTC(1/5)
Bsp1407I 3293 TGTACA T^GTACA
BspEI 6017 TCCGGA T^CCGGA
BspLU11I 385 ACATGT A^CATGT
BsrGI 3293 TGTACA T^GTACA
BstEII 3165 GGTGACC G^GT[ACGT]ACC
BstPI 3165 GGTGACC G^GT[ACGT]ACC
DraI 3715 TTTAAA TTT^AAA
DraIII 1853 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 5114 CGGCCG C^GGCCG
Eam1105I 5788 GACCCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 5114 CGGCCG C^GGCCG
Eco52I 5114 CGGCCG C^GGCCG
EcoO65I 3165 GGTGACC G^GT[ACGT]ACC
EcoRV 6091 GATATC GAT^ATC
EcoT22I 224 ATGCAT ATGCA^T
HindIII 6084 AAGCTT A^AGCTT
KpnI 321 GGTACC GGTAC^C
MroI 6017 TCCGGA T^CCGGA
NheI 5070 GCTAGC G^CTAGC
NsiI 224 ATGCAT ATGCA^T
Psp1406I 1288 AACGTT AA^CGTT
SpeI 1335 ACTAGT A^CTAGT
SphI 671 GCATGC GCATG^C
SspBI 3293 TGTACA T^GTACA
XmnI 4973 GAATACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 648 CGCG CG^CG
3854 CGCG
AflII 4919 CTTAAG C^TTAAG
5080 CTTAAG
Aor51HI 2571 AGCGCT AGC^GCT
5944 AGCGCT
AspI 1552 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4002 GACAAGGTC
AvrII 3435 CCTAGG C^CTAGG
4778 CCTAGG
BalI 5702 TGGCCA TGG^CCA
5824 TGGCCA
BbeI 4473 GGCGCC GGCGC^C
5779 GGCGCC
BfrI 4919 CTTAAG C^TTAAG
5080 CTTAAG
BglI 1404 GCCACACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1501 GCCATGGTGGC
BlnI 3435 CCTAGG C^CTAGG
4778 CCTAGG
BlpI 708 GCTCAGC GC^T[ACGT]AGC
1375 GCTGAGC
Bpu1102I 708 GCTCAGC GC^T[ACGT]AGC
1375 GCTGAGC
BsmI 1032 GCATTC GAATGC(1/-1)
4672 GCATTC
BspHI 614 TCATGA T^CATGA
3216 TCATGA
BstAPI 990 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4950 GCATCCTGTGC
BstBI 1702 TTCGAA TT^CGAA
1969 TTCGAA
BstUI 648 CGCG CG^CG
3854 CGCG
CelII 708 GCTCAGC GC^T[ACGT]AGC
1375 GCTGAGC
DrdI 1219 GACCAGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2743 GACATTGGCGTC
Eco47III 2571 AGCGCT AGC^GCT
5944 AGCGCT
EcoRI 1804 GAATTC G^AATTC
2703 GAATTC
KasI 4473 GGCGCC G^GCGCC
5779 GGCGCC
MluNI 5702 TGGCCA TGG^CCA
5824 TGGCCA
MscI 5702 TGGCCA TGG^CCA
5824 TGGCCA
MvnI 648 CGCG CG^CG
3854 CGCG
NarI 4473 GGCGCC GG^CGCC
5779 GGCGCC
NspV 1702 TTCGAA TT^CGAA
1969 TTCGAA
PflFI 1552 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4002 GACAAGGTC
RcaI 614 TCATGA T^CATGA
3216 TCATGA
ScaI 1489 AGTACT AGT^ACT
5019 AGTACT
SfoI 4473 GGCGCC GGC^GCC
5779 GGCGCC
SfuI 1702 TTCGAA TT^CGAA
1969 TTCGAA
Tth111I 1552 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4002 GACAAGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII BanI BanII
BbrPI BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI