##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AccIII AclI AgeI AscI
AseI AsnI Asp718I AviII AvrII
BcgI BciVI BlnI BsaBI BseAI
BsiWI BspDI BspEI BsrDI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
EcoO65I EcoRI EcoT22I FseI FspI
HindIII HpaI KpnI KspI MamI
MfeI MroI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I SacII SapI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AatII 3138 GACGTC GACGT^C
AccI 2159 GTCGAC GT^[AC][GT]AC
AflII 5076 CTTAAG C^TTAAG
Alw44I 447 GTGCAC G^TGCAC
ApaLI 447 GTGCAC G^TGCAC
Asp700I 2141 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 2041 GGATCC G^GATCC
BbrPI 453 CACGTG CAC^GTG
BfrI 5076 CTTAAG C^TTAAG
BglII 1576 AGATCT A^GATCT
BsaAI 453 CACGTG [CT]AC^GT[GA]
BsmI 853 GCATTC GAATGC(1/-1)
Bsp1407I 4656 TGTACA T^GTACA
BspHI 4993 TCATGA T^CATGA
BspLU11I 4162 ACATGT A^CATGT
BsrGI 4656 TGTACA T^GTACA
CpoI 3970 CGGTCCG CG^G[AT]CCG
DraI 215 TTTAAA TTT^AAA
DrdI 993 GACGCCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
MluI 2163 ACGCGT A^CGCGT
PmaCI 453 CACGTG CAC^GTG
PmlI 453 CACGTG CAC^GTG
PvuI 24 CGATCG CGAT^CG
RcaI 4993 TCATGA T^CATGA
RsrII 3970 CGGTCCG CG^G[AT]CCG
SalI 2159 GTCGAC G^TCGAC
SspBI 4656 TGTACA T^GTACA
XmnI 2141 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AocI 1028 CCTCAGG CC^T[ACGT]AGG
3498 CCTCAGG
Aor51HI 2272 AGCGCT AGC^GCT
2839 AGCGCT
BbeI 1979 GGCGCC GGCGC^C
4371 GGCGCC
BclI 799 TGATCA T^GATCA
1327 TGATCA
BglI 148 GCCCGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1395 GCCTGGATGGC
BsaWI 634 ACCGGA [AT]^CCGG[AT]
4065 TCCGGT
Bsu36I 1028 CCTCAGG CC^T[ACGT]AGG
3498 CCTCAGG
EagI 2755 CGGCCG C^GGCCG
3350 CGGCCG
EclXI 2755 CGGCCG C^GGCCG
3350 CGGCCG
Eco47III 2272 AGCGCT AGC^GCT
2839 AGCGCT
Eco52I 2755 CGGCCG C^GGCCG
3350 CGGCCG
Eco81I 1028 CCTCAGG CC^T[ACGT]AGG
3498 CCTCAGG
EcoRV 975 GATATC GAT^ATC
1173 GATATC
FbaI 799 TGATCA T^GATCA
1327 TGATCA
HincII 2159 GTCGAC GT[CT]^[GA]AC
4931 GTTGAC
HindII 2159 GTCGAC GT[CT]^[GA]AC
4931 GTTGAC
KasI 1979 GGCGCC G^GCGCC
4371 GGCGCC
MslI 3237 CATCCGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4266 CACTCCTGTG
NarI 1979 GGCGCC GG^CGCC
4371 GGCGCC
SbfI 497 CCTGCAGG CCTGCA^GG
1664 CCTGCAGG
SfoI 1979 GGCGCC GGC^GCC
4371 GGCGCC
Sse8387I 497 CCTGCAGG CCTGCA^GG
1664 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
ApaI ApoI AspEI AspHI AspI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssHII BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI Eam1105I EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SphI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmaCI XmaI