##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AclI AcyI AflII AhdI
Aor51HI AscI AseI AsnI AspEI
AviII BbeI BbsI BfrI BpuAI
BsaBI BsaHI BsiWI BsmBI BspDI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I ClaI DraIII Eam1105I
Eco47III EcoO65I FseI FspI Hin1I
HpaI KasI MamI MluI NarI
NheI NotI NruI NspV PacI
PaeR7I PmeI PshAI PshBI Psp1406I
PvuI SacI SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 3507 GGTACC G^GTACC
AgeI 3948 ACCGGT A^CCGGT
Asp718I 3507 GGTACC G^GTACC
AspI 1063 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1817 GGATCC G^GATCC
BbvCI 4352 GCTGAGG CCTCAGC(-5/-2)
BciVI 3874 GGATAC GTATCC(6/5)
BclI 1322 TGATCA T^GATCA
BglI 3384 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3767 AGATCT A^GATCT
BlpI 214 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 214 GCTGAGC GC^T[ACGT]AGC
BsiEI 1272 CGGCCG CG[GA][CT]^CG
BsrDI 3358 GCAATG GCAATG(2/0)
CelII 214 GCTGAGC GC^T[ACGT]AGC
CpoI 409 CGGACCG CG^G[AT]CCG
DraI 4102 TTTAAA TTT^AAA
EagI 1272 CGGCCG C^GGCCG
EclXI 1272 CGGCCG C^GGCCG
Eco52I 1272 CGGCCG C^GGCCG
EcoNI 474 CCTGATACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2905 GATATC GAT^ATC
FbaI 1322 TGATCA T^GATCA
HaeII 1277 GGCGCT [GA]GCGC^[CT]
HincII 1069 GTCAAC GT[CT]^[GA]AC
HindII 1069 GTCAAC GT[CT]^[GA]AC
KpnI 3507 GGTACC GGTAC^C
KspI 423 CCGCGG CCGC^GG
MfeI 3269 CAATTG C^AATTG
MunI 3269 CAATTG C^AATTG
NdeI 4759 CATATG CA^TATG
PflFI 1063 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3948 ACCGGT A^CCGGT
PstI 385 CTGCAG CTGCA^G
RsrII 409 CGGACCG CG^G[AT]CCG
SacII 423 CCGCGG CCGC^GG
SphI 795 GCATGC GCATG^C
SspI 4516 AATATT AAT^ATT
Tth111I 1063 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 962 TCCGGA T^CCGGA
1337 TCCGGA
Alw44I 1141 GTGCAC G^TGCAC
2167 GTGCAC
ApaLI 1141 GTGCAC G^TGCAC
2167 GTGCAC
AvaI 73 CCCGAG C^[CT]CG[GA]G
507 CTCGGG
AvrII 2523 CCTAGG C^CTAGG
2774 CCTAGG
BcgI 2682 CGATCCGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
684 GCAAGACACTCG
BlnI 2523 CCTAGG C^CTAGG
2774 CCTAGG
BseAI 962 TCCGGA T^CCGGA
1337 TCCGGA
BsgI 2081 CTGCAC GTGCAG(16/14)
3590 GTGCAG
BsoBI 73 CCCGAG C^[CT]CG[GA]G
507 CTCGGG
Bsp1407I 697 TGTACA T^GTACA
2366 TGTACA
BspEI 962 TCCGGA T^CCGGA
1337 TCCGGA
BspLU11I 4037 ACATGT A^CATGT
4794 ACATGT
BsrBI 217 GAGCGG CCGCTC(-3/-3)
1222 GAGCGG
BsrGI 697 TGTACA T^GTACA
2366 TGTACA
BssSI 910 CACGAG CACGAG(-5/-1)
1083 CTCGTG
DrdI 1860 GACTTGCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2519 GACCCCTAGGTC
EcoT22I 3937 ATGCAT ATGCA^T
4283 ATGCAT
MroI 962 TCCGGA T^CCGGA
1337 TCCGGA
NaeI 235 GCCGGC GCC^GGC
1274 GCCGGC
NgoMIV 235 GCCGGC G^CCGGC
1274 GCCGGC
NsiI 3937 ATGCAT ATGCA^T
4283 ATGCAT
SapI 1044 GAAGAGC GCTCTTC(1/4)
3700 GAAGAGC
SspBI 697 TGTACA T^GTACA
2366 TGTACA
StuI 1409 AGGCCT AGG^CCT
2771 AGGCCT
TaqI 1879 TCGA T^CGA
2188 TCGA
TthHB8I 1879 TCGA T^CGA
2188 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI Asp700I AspHI AvaII
BalI BanI BanII BbrPI BbvI
BcnI BfaI BmyI BpmI BsaAI
BsaI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspHI BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PmaCI PmlI PpuMI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XcmI XhoII XmnI