##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Aor51HI Asp700I AviII BamHI BbrPI
BcgI BfrI BsaWI BseAI BsiWI
BsmBI BspDI BspEI BsrBI BsrDI
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DraIII Eco47III EcoRI EcoRV
FseI FspI HpaI KspI MfeI
MluI MroI MunI NdeI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SalI SapI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 4402 GGTACC G^GTACC
AccI 4758 GTAGAC GT^[AC][GT]AC
AhdI 3237 GACAGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 673 GTGCAC G^TGCAC
AocI 4254 CCTAAGG CC^T[ACGT]AGG
ApaLI 673 GTGCAC G^TGCAC
AscI 277 GGCGCGCC GG^CGCGCC
Asp718I 4402 GGTACC G^GTACC
AspEI 3237 GACAGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3382 CCTAGG C^CTAGG
BbeI 184 GGCGCC GGCGC^C
BciVI 4816 GTATCC GTATCC(6/5)
BclI 1645 TGATCA T^GATCA
BlnI 3382 CCTAGG C^CTAGG
BlpI 3701 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3701 GCTCAGC GC^T[ACGT]AGC
BsaAI 911 TACGTG [CT]AC^GT[GA]
BsaBI 4527 GATCCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 35 CGGCCG CG[GA][CT]^CG
Bsp1407I 2238 TGTACA T^GTACA
BsrGI 2238 TGTACA T^GTACA
BstAPI 2690 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3663 GGTAACC G^GT[ACGT]ACC
BstPI 3663 GGTAACC G^GT[ACGT]ACC
Bsu36I 4254 CCTAAGG CC^T[ACGT]AGG
CelII 3701 GCTCAGC GC^T[ACGT]AGC
DrdI 1160 GACCGTCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1863 CCATGG C^C[GA][CT]GG
EagI 35 CGGCCG C^GGCCG
Eam1105I 3237 GACAGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 35 CGGCCG C^GGCCG
Eco52I 35 CGGCCG C^GGCCG
Eco81I 4254 CCTAAGG CC^T[ACGT]AGG
EcoO65I 3663 GGTAACC G^GT[ACGT]ACC
EcoT22I 4900 ATGCAT ATGCA^T
FbaI 1645 TGATCA T^GATCA
HincII 1825 GTCAAC GT[CT]^[GA]AC
HindII 1825 GTCAAC GT[CT]^[GA]AC
KasI 184 GGCGCC G^GCGCC
KpnI 4402 GGTACC GGTAC^C
MamI 4527 GATCCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 37 GCCGGC GCC^GGC
NarI 184 GGCGCC GG^CGCC
NcoI 1863 CCATGG C^CATGG
NgoMIV 37 GCCGGC G^CCGGC
NsiI 4900 ATGCAT ATGCA^T
SbfI 868 CCTGCAGG CCTGCA^GG
SexAI 3525 ACCAGGT A^CC[AT]GGT
SfoI 184 GGCGCC GGC^GCC
SphI 2756 GCATGC GCATG^C
Sse8387I 868 CCTGCAGG CCTGCA^GG
SspBI 2238 TGTACA T^GTACA
StuI 1002 AGGCCT AGG^CCT
XbaI 3114 TCTAGA T^CTAGA
XcmI 1890 CCAGCTCCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 184 GGCGCC G[GA]^CG[CT]C
459 GACGCC
AlwNI 783 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
4501 CAGAGACTG
ApaI 2563 GGGCCC GGGCC^C
3840 GGGCCC
BglII 1064 AGATCT A^GATCT
4363 AGATCT
BsaHI 184 GGCGCC G[GA]^CG[CT]C
459 GACGCC
BsmI 1774 GAATGC GAATGC(1/-1)
4147 GAATGC
BspLU11I 4083 ACATGT A^CATGT
4277 ACATGT
BsrFI 37 GCCGGC [GA]^CCGG[CT]
881 ACCGGC
BssHII 99 GCGCGC G^CGCGC
278 GCGCGC
Cfr10I 37 GCCGGC [GA]^CCGG[CT]
881 ACCGGC
DraI 3300 TTTAAA TTT^AAA
4982 TTTAAA
EcoNI 994 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3436 CCTCTCTCAGG
Hin1I 184 GGCGCC G[GA]^CG[CT]C
459 GACGCC
MaeII 912 ACGT A^CGT
1629 ACGT
NheI 41 GCTAGC G^CTAGC
688 GCTAGC
PpuMI 6 GGGTCCC [GA]G^G[AT]CC[CT]
430 GGGACCC
PshAI 1118 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2111 GACGGATGTC
SmlI 2846 CTCAAG C^T[CT][GA]AG
3888 CTCAAG
SspI 3268 AATATT AAT^ATT
4660 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
AspHI AspI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PshBI PstI PvuII RcaI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XhoII XmaCI
XmaI