##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccII AclI AgeI
AhdI Aor51HI AscI Asp718I AspEI
AviII BalI BamHI BbrPI BcgI
BclI BglI BsaAI BsgI BsiEI
BsiWI BsmBI BspHI BspMI BsrBI
BsrFI BssHII BssSI BstBI BstEII
BstPI BstUI BstXI Cfr10I CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I FauI FbaI FseI
FspI HpaI KpnI KspI MluI
MluNI MscI MvnI NaeI NgoMIV
NheI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI PvuII RcaI RsrII
SacII SapI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AccIII 2585 TCCGGA T^CCGGA
AciI 1306 GCGG CCGC(-3/-1)
Alw44I 1115 GTGCAC G^TGCAC
AocI 3008 CCTGAGG CC^T[ACGT]AGG
ApaI 994 GGGCCC GGGCC^C
ApaLI 1115 GTGCAC G^TGCAC
Asp700I 2356 GAATTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3717 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1169 CTCGAG C^[CT]CG[GA]G
AvrII 1951 CCTAGG C^CTAGG
BbeI 44 GGCGCC GGCGC^C
BbvCI 3495 GCTGAGG CCTCAGC(-5/-2)
BglII 1975 AGATCT A^GATCT
BlnI 1951 CCTAGG C^CTAGG
BlpI 1739 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1739 GCTTAGC GC^T[ACGT]AGC
BsaI 4 GAGACC GGTCTC(1/5)
BsaWI 2585 TCCGGA [AT]^CCGG[AT]
BseAI 2585 TCCGGA T^CCGGA
BsmFI 3434 GGGAC GGGAC(10/14)
BsoBI 1169 CTCGAG C^[CT]CG[GA]G
BspDI 413 ATCGAT AT^CGAT
BspEI 2585 TCCGGA T^CCGGA
BspLU11I 2696 ACATGT A^CATGT
Bst1107I 3962 GTATAC GTA^TAC
BstAPI 253 GCAAATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3962 GTATAC GTA^TAC
Bsu36I 3008 CCTGAGG CC^T[ACGT]AGG
Cac8I 717 GCGAGC GC[ACGT]^[ACGT]GC
CelII 1739 GCTTAGC GC^T[ACGT]AGC
CfoI 45 GCGC GCG^C
ClaI 413 ATCGAT AT^CGAT
EaeI 38 TGGCCG [CT]^GGCC[GA]
Eco81I 3008 CCTGAGG CC^T[ACGT]AGG
EcoNI 2107 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1914 GATATC GAT^ATC
EcoT22I 1175 ATGCAT ATGCA^T
HaeII 44 GGCGCC [GA]GCGC^[CT]
HhaI 45 GCGC GCG^C
HinP1I 45 GCGC G^CGC
HindIII 1129 AAGCTT A^AGCTT
KasI 44 GGCGCC G^GCGCC
MfeI 1871 CAATTG C^AATTG
MroI 2585 TCCGGA T^CCGGA
MslI 1752 CACAGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1304 CAGCGG C[AC]G^C[GT]G
MunI 1871 CAATTG C^AATTG
NarI 44 GGCGCC GG^CGCC
NcoI 2069 CCATGG C^CATGG
NsiI 1175 ATGCAT ATGCA^T
PaeR7I 1169 CTCGAG C^TCGAG
PflFI 3717 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1777 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 852 AGGACCC [GA]G^G[AT]CC[CT]
ScaI 639 AGTACT AGT^ACT
SfoI 44 GGCGCC GGC^GCC
SwaI 407 ATTTAAAT ATTT^AAAT
Tth111I 3717 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1777 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3197 TCTAGA T^CTAGA
XhoI 1169 CTCGAG C^TCGAG
XmnI 2356 GAATTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 44 GGCGCC G[GA]^CG[CT]C
3988 GACGCC
AflII 139 CTTAAG C^TTAAG
4096 CTTAAG
AlwNI 1304 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
3203 CAGGCCCTG
AseI 2358 ATTAAT AT^TAAT
3072 ATTAAT
AsnI 2358 ATTAAT AT^TAAT
3072 ATTAAT
BanI 44 GGCGCC G^G[CT][GA]CC
1339 GGCACC
BciVI 359 GTATCC GTATCC(6/5)
1142 GTATCC
BcnI 41 CCGGG CC^[GC]GG
2146 CCCGG
BfrI 139 CTTAAG C^TTAAG
4096 CTTAAG
BsaHI 44 GGCGCC G[GA]^CG[CT]C
3988 GACGCC
BseRI 2105 CTCCTC GAGGAG(10/8)
2738 GAGGAG
Bsp1407I 275 TGTACA T^GTACA
1092 TGTACA
BsrDI 2444 CATTGC GCAATG(2/0)
2782 GCAATG
BsrGI 275 TGTACA T^GTACA
1092 TGTACA
BstYI 825 AGATCC [GA]^GATC[CT]
1975 AGATCT
DraIII 1388 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
2603 CACCTTGTG
Hin1I 44 GGCGCC G[GA]^CG[CT]C
3988 GACGCC
MflI 825 AGATCC [GA]^GATC[CT]
1975 AGATCT
NciI 41 CCGGG CC^[GC]GG
2146 CCCGG
PshBI 2358 ATTAAT AT^TAAT
3072 ATTAAT
SacI 2792 GAGCTC GAGCT^C
3030 GAGCTC
SalI 911 GTCGAC G^TCGAC
3985 GTCGAC
SspBI 275 TGTACA T^GTACA
1092 TGTACA
XcmI 529 CCAGAATATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
953 CCAAAACTGGAATGG
XhoII 825 AGATCC [GA]^GATC[CT]
1975 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaII BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaBI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmI Bsp1286I BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII DraI DraII DsaI EarI
EcoO109I EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MnlI MseI MspI
MvaI MwoI NdeI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I