##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccIII AcyI AflII AgeI
AhdI AscI AspEI AvrII BbeI
BbvCI BfrI BlnI BsaHI BsaWI
BseAI BsiWI BspDI BspEI BsrDI
BssSI Bst1107I BstEII BstPI BstZ17I
ClaI CpoI DraIII Eam1105I EcoNI
EcoO65I FseI Hin1I HincII HindII
HpaI KasI MfeI MluI MroI
MunI NarI NheI NotI PacI
PinAI PmeI PshAI PvuI RsrII
SacI SalI SbfI ScaI SexAI
SfoI SgrAI SnaBI SphI Sse8387I
StuI SwaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 5280 GGTACC G^GTACC
AccI 6053 GTAGAC GT^[AC][GT]AC
AclI 6201 AACGTT AA^CGTT
Aor51HI 3052 AGCGCT AGC^GCT
ApaI 3115 GGGCCC GGGCC^C
Asp718I 5280 GGTACC G^GTACC
AviII 55 TGCGCA TGC^GCA
BalI 3106 TGGCCA TGG^CCA
BbrPI 942 CACGTG CAC^GTG
BciVI 813 GTATCC GTATCC(6/5)
BsaBI 2577 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BstBI 6273 TTCGAA TT^CGAA
DrdI 6700 GACAAACTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3052 AGCGCT AGC^GCT
EcoT22I 559 ATGCAT ATGCA^T
FspI 55 TGCGCA TGC^GCA
KpnI 5280 GGTACC GGTAC^C
KspI 440 CCGCGG CCGC^GG
MamI 2577 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3106 TGGCCA TGG^CCA
MscI 3106 TGGCCA TGG^CCA
NruI 226 TCGCGA TCG^CGA
NsiI 559 ATGCAT ATGCA^T
NspV 6273 TTCGAA TT^CGAA
PaeR7I 2190 CTCGAG C^TCGAG
PmaCI 942 CACGTG CAC^GTG
PmlI 942 CACGTG CAC^GTG
PpuMI 5022 GGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 6201 AACGTT AA^CGTT
SacII 440 CCGCGG CCGC^GG
SfuI 6273 TTCGAA TT^CGAA
SpeI 3968 ACTAGT A^CTAGT
XcmI 817 CCAAGACCAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2190 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 872 GTGCAC G^TGCAC
3554 GTGCAC
AocI 897 CCTGAGG CC^T[ACGT]AGG
1497 CCTGAGG
ApaLI 872 GTGCAC G^TGCAC
3554 GTGCAC
AseI 3281 ATTAAT AT^TAAT
5144 ATTAAT
AsnI 3281 ATTAAT AT^TAAT
5144 ATTAAT
Asp700I 3884 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4292 GAATTCTTTC
AspI 1307 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2735 GACAGTGTC
BamHI 374 GGATCC G^GATCC
5562 GGATCC
BclI 3626 TGATCA T^GATCA
5454 TGATCA
BsaAI 942 CACGTG [CT]AC^GT[GA]
3259 CACGTA
BsaI 477 GAGACC GGTCTC(1/5)
1305 GAGACC
BsmBI 1782 CGTCTC CGTCTC(1/5)
2533 GAGACG
BspLU11I 1193 ACATGT A^CATGT
6603 ACATGT
BssHII 10 GCGCGC G^CGCGC
3010 GCGCGC
BstXI 3097 CCAAGAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4349 CCACATAGATGG
Bsu36I 897 CCTGAGG CC^T[ACGT]AGG
1497 CCTGAGG
Eco81I 897 CCTGAGG CC^T[ACGT]AGG
1497 CCTGAGG
EcoRI 4292 GAATTC G^AATTC
5988 GAATTC
EcoRV 711 GATATC GAT^ATC
5922 GATATC
FbaI 3626 TGATCA T^GATCA
5454 TGATCA
PflFI 1307 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2735 GACAGTGTC
PshBI 3281 ATTAAT AT^TAAT
5144 ATTAAT
SapI 2364 GAAGAGC GCTCTTC(1/4)
3211 GCTCTTC
SfiI 3086 GGCCGTGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3107 GGCCAGAAGGGCC
Tth111I 1307 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2735 GACAGTGTC
XmnI 3884 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4292 GAATTCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcgI BcnI BfaI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspHI
BspMI BsrBI BsrFI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XhoII XmaCI
XmaI