##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AccII AclI AgeI Aor51HI
AscI AviII BamHI BbeI BbrPI
BbvCI BcgI BsiEI BsiWI BspDI
BsrBI BssHII BstBI BstUI ClaI
CpoI DraIII DrdI EagI EclXI
Eco47III Eco52I FauI FseI FspI
HpaI KasI KspI MluI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacI SacII SalI SbfI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 3964 GGTACC G^GTACC
AccIII 2748 TCCGGA T^CCGGA
AcyI 3919 GGCGTC G[GA]^CG[CT]C
AflII 6181 CTTAAG C^TTAAG
Asp718I 3964 GGTACC G^GTACC
AspI 4971 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 786 GGATAC GTATCC(6/5)
BclI 4155 TGATCA T^GATCA
BfrI 6181 CTTAAG C^TTAAG
BglI 3763 GCCGCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 3439 TACGTG [CT]AC^GT[GA]
BsaHI 3919 GGCGTC G[GA]^CG[CT]C
BsaWI 2748 TCCGGA [AT]^CCGG[AT]
BseAI 2748 TCCGGA T^CCGGA
BsmBI 3921 CGTCTC CGTCTC(1/5)
BspEI 2748 TCCGGA T^CCGGA
BssSI 82 CTCGTG CACGAG(-5/-1)
BstAPI 3732 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 5946 GGTTACC G^GT[ACGT]ACC
BstPI 5946 GGTTACC G^GT[ACGT]ACC
EcoO65I 5946 GGTTACC G^GT[ACGT]ACC
EcoRV 73 GATATC GAT^ATC
FbaI 4155 TGATCA T^GATCA
HgaI 3920 GCGTC GACGC(5/10)
Hin1I 3919 GGCGTC G[GA]^CG[CT]C
KpnI 3964 GGTACC GGTAC^C
MfeI 4139 CAATTG C^AATTG
MroI 2748 TCCGGA T^CCGGA
MunI 4139 CAATTG C^AATTG
PaeR7I 2603 CTCGAG C^TCGAG
PflFI 4971 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4824 CCATTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI 2364 AGTACT AGT^ACT
Tth111I 4971 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4824 CCATTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2603 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AhdI 4439 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4731 GACATCTTGTC
AocI 709 CCTGAGG CC^T[ACGT]AGG
4893 CCTGAGG
AspEI 4439 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4731 GACATCTTGTC
AvrII 5123 CCTAGG C^CTAGG
5475 CCTAGG
BalI 777 TGGCCA TGG^CCA
5374 TGGCCA
BanI 1281 GGTGCC G^G[CT][GA]CC
3964 GGTACC
BlnI 5123 CCTAGG C^CTAGG
5475 CCTAGG
BlpI 3070 GCTGAGC GC^T[ACGT]AGC
4285 GCTGAGC
Bpu1102I 3070 GCTGAGC GC^T[ACGT]AGC
4285 GCTGAGC
BsaBI 73 GATATCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1017 GATTATCATC
BsmI 6078 GAATGC GAATGC(1/-1)
6345 GCATTC
BspHI 1388 TCATGA T^CATGA
2718 TCATGA
BspLU11I 437 ACATGT A^CATGT
6429 ACATGT
BsrDI 4986 CATTGC GCAATG(2/0)
3751 GCAATG
BsrFI 4016 ACCGGC [GA]^CCGG[CT]
4046 ACCGGC
Bst1107I 2141 GTATAC GTA^TAC
3457 GTATAC
BstZ17I 2141 GTATAC GTA^TAC
3457 GTATAC
Bsu36I 709 CCTGAGG CC^T[ACGT]AGG
4893 CCTGAGG
CelII 3070 GCTGAGC GC^T[ACGT]AGC
4285 GCTGAGC
Cfr10I 4016 ACCGGC [GA]^CCGG[CT]
4046 ACCGGC
EaeI 777 TGGCCA [CT]^GGCC[GA]
5374 TGGCCA
Eam1105I 4439 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4731 GACATCTTGTC
EarI 4528 CTCTTC CTCTTC(1/4)
3142 GAAGAG
Eco81I 709 CCTGAGG CC^T[ACGT]AGG
4893 CCTGAGG
EcoNI 2413 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4083 CCTGGGCGAGG
EcoRI 310 GAATTC G^AATTC
1648 GAATTC
EcoT22I 2372 ATGCAT ATGCA^T
5284 ATGCAT
HincII 244 GTTGAC GT[CT]^[GA]AC
6551 GTCAAC
HindII 244 GTTGAC GT[CT]^[GA]AC
6551 GTCAAC
HindIII 686 AAGCTT A^AGCTT
1916 AAGCTT
Ksp632I 4528 CTCTTC CTCTTC(1/4)
3142 GAAGAG
MamI 73 GATATCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1017 GATTATCATC
MluNI 777 TGGCCA TGG^CCA
5374 TGGCCA
MscI 777 TGGCCA TGG^CCA
5374 TGGCCA
NdeI 1680 CATATG CA^TATG
1772 CATATG
NsiI 2372 ATGCAT ATGCA^T
5284 ATGCAT
RcaI 1388 TCATGA T^CATGA
2718 TCATGA
SapI 3142 GAAGAGC GCTCTTC(1/4)
4527 GCTCTTC
SexAI 4829 ACCTGGT A^CC[AT]GGT
6702 ACCAGGT
SmaI 3117 CCCGGG CCC^GGG
3893 CCCGGG
XmaCI 3117 CCCGGG C^CCGGG
3893 CCCGGG
XmaI 3117 CCCGGG C^CCGGG
3893 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI ApoI AseI AsnI Asp700I
AspHI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PshAI PshBI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XcmI
XhoII XmnI