##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AccII AgeI AhdI ApaI AscI
AspEI AviII AvrII BamHI BbvCI
BglI BlnI BsiEI BsiWI BsmBI
BspDI BspLU11I BsrBI BsrFI BssHII
BstBI BstEII BstPI BstUI Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRV FseI
FspI HgaI HpaI KspI MluI
MvnI NaeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshAI PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI Sse8387I
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 604 GACGTC GACGT^C
Acc65I 529 GGTACC G^GTACC
AccIII 3052 TCCGGA T^CCGGA
AclI 5761 AACGTT AA^CGTT
AflIII 1815 ACACGT A^C[GA][CT]GT
Aor51HI 3835 AGCGCT AGC^GCT
AseI 4209 ATTAAT AT^TAAT
AsnI 4209 ATTAAT AT^TAAT
Asp700I 988 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 529 GGTACC G^GTACC
AspI 417 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3009 CTCGGG C^[CT]CG[GA]G
AvaII 3688 GGACC G^G[AT]CC
BcgI 4536 GCAAAGTATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 6000 TGATCA T^GATCA
BsaWI 3052 TCCGGA [AT]^CCGG[AT]
BseAI 3052 TCCGGA T^CCGGA
BsoBI 3009 CTCGGG C^[CT]CG[GA]G
BspEI 3052 TCCGGA T^CCGGA
BssSI 3062 CTCGTG CACGAG(-5/-1)
Bst1107I 6081 GTATAC GTA^TAC
BstXI 2340 CCAGAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 6081 GTATAC GTA^TAC
DraII 3687 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1864 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 788 CCATGG C^C[GA][CT]GG
Eco47III 3835 AGCGCT AGC^GCT
EcoO109I 3687 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 2107 GAATTC G^AATTC
FbaI 6000 TGATCA T^GATCA
KpnI 529 GGTACC GGTAC^C
MroI 3052 TCCGGA T^CCGGA
NcoI 788 CCATGG C^CATGG
NspI 2481 GCATGC [GA]CATG^[CT]
PflFI 417 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4723 CCATATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 3687 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 4209 ATTAAT AT^TAAT
Psp1406I 5761 AACGTT AA^CGTT
SpeI 4447 ACTAGT A^CTAGT
SphI 2481 GCATGC GCATG^C
Tth111I 417 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4723 CCATATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2754 TCTAGA T^CTAGA
XmnI 988 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 167 GTAGAC GT^[AC][GT]AC
6081 GTATAC
AocI 44 CCTCAGG CC^T[ACGT]AGG
658 CCTCAGG
BalI 2880 TGGCCA TGG^CCA
3150 TGGCCA
BbeI 2329 GGCGCC GGCGC^C
4440 GGCGCC
BciVI 3815 GTATCC GTATCC(6/5)
4673 GTATCC
BlpI 322 GCTGAGC GC^T[ACGT]AGC
1624 GCTGAGC
Bpu1102I 322 GCTGAGC GC^T[ACGT]AGC
1624 GCTGAGC
BsaBI 2029 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4972 GATAGAAATC
BspHI 968 TCATGA T^CATGA
3410 TCATGA
Bsu36I 44 CCTCAGG CC^T[ACGT]AGG
658 CCTCAGG
CelII 322 GCTGAGC GC^T[ACGT]AGC
1624 GCTGAGC
Eco81I 44 CCTCAGG CC^T[ACGT]AGG
658 CCTCAGG
KasI 2329 GGCGCC G^GCGCC
4440 GGCGCC
MamI 2029 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4972 GATAGAAATC
MfeI 1610 CAATTG C^AATTG
1994 CAATTG
MluNI 2880 TGGCCA TGG^CCA
3150 TGGCCA
MscI 2880 TGGCCA TGG^CCA
3150 TGGCCA
MunI 1610 CAATTG C^AATTG
1994 CAATTG
NarI 2329 GGCGCC GG^CGCC
4440 GGCGCC
RcaI 968 TCATGA T^CATGA
3410 TCATGA
ScaI 617 AGTACT AGT^ACT
5715 AGTACT
SfoI 2329 GGCGCC GGC^GCC
4440 GGCGCC
StuI 1762 AGGCCT AGG^CCT
2789 AGGCCT
SwaI 1127 ATTTAAAT ATTT^AAAT
4230 ATTTAAAT
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflII
AluI Alw44I AlwI AlwNI ApaLI
ApoI AspHI BanI BanII BbrPI
BbsI BbvI BcnI BfaI BfrI
BglII BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspMI BsrDI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI EaeI EarI
EcoNI EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeI NdeII NlaIII NlaIV NsiI
PleI PmaCI PmlI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII