##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AseI AsnI Asp700I AspI AvrII
BamHI BfrI BglII BlnI BsaBI
BsaWI BseAI BsiWI BspEI BspHI
CpoI DraI EcoT22I FseI HincII
HindII HpaI MamI MfeI MluI
MroI MseI MunI NdeI NruI
NsiI PacI PaeR7I PflFI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SalI SgrAI SnaBI SpeI
SspI SwaI TfiI Tru9I Tth111I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 4515 GAATTC [GA]^AATT[CT]
ApoI 4515 GAATTC [GA]^AATT[CT]
AscI 3194 GGCGCGCC GG^CGCGCC
BclI 5593 TGATCA T^GATCA
BspDI 3840 ATCGAT AT^CGAT
BspLU11I 1546 ACATGT A^CATGT
BsrDI 4115 CATTGC GCAATG(2/0)
Bst1107I 3699 GTATAC GTA^TAC
BstBI 3036 TTCGAA TT^CGAA
BstZ17I 3699 GTATAC GTA^TAC
ClaI 3840 ATCGAT AT^CGAT
DrdI 1740 GACGTGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 4515 GAATTC G^AATTC
EcoRV 4079 GATATC GAT^ATC
FbaI 5593 TGATCA T^GATCA
HindIII 4289 AAGCTT A^AGCTT
NheI 5024 GCTAGC G^CTAGC
NotI 6357 GCGGCCGC GC^GGCCGC
NspV 3036 TTCGAA TT^CGAA
PshAI 3887 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 4093 AGTACT AGT^ACT
SfiI 6172 GGCCCGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 3036 TTCGAA TT^CGAA
Tsp509I 4516 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 188 GGTACC G^GTACC
2855 GGTACC
Alw44I 383 GTGCAC G^TGCAC
878 GTGCAC
ApaLI 383 GTGCAC G^TGCAC
878 GTGCAC
Asp718I 188 GGTACC G^GTACC
2855 GGTACC
BciVI 257 GGATAC GTATCC(6/5)
597 GGATAC
BsmBI 897 GAGACG CGTCTC(1/5)
6096 GAGACG
BsmI 3086 GAATGC GAATGC(1/-1)
4983 GCATTC
Bsp1407I 197 TGTACA T^GTACA
307 TGTACA
BsrGI 197 TGTACA T^GTACA
307 TGTACA
BstYI 1798 AGATCC [GA]^GATC[CT]
5574 GGATCT
KpnI 188 GGTACC GGTAC^C
2855 GGTACC
KspI 2046 CCGCGG CCGC^GG
5612 CCGCGG
MflI 1798 AGATCC [GA]^GATC[CT]
5574 GGATCT
NaeI 478 GCCGGC GCC^GGC
6317 GCCGGC
NgoMIV 478 GCCGGC G^CCGGC
6317 GCCGGC
SacII 2046 CCGCGG CCGC^GG
5612 CCGCGG
SapI 1484 GCTCTTC GCTCTTC(1/4)
3830 GCTCTTC
SexAI 496 ACCTGGT A^CC[AT]GGT
3961 ACCAGGT
SspBI 197 TGTACA T^GTACA
307 TGTACA
XhoII 1798 AGATCC [GA]^GATC[CT]
5574 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 156 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
AocI Aor51HI ApaI AspEI AspHI
AvaI AvaII AviII BalI BanI
BanII BbeI BbrPI BbsI BbvCI
BbvI BcgI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssHII BssKI
BssSI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SfoI SmaI SmlI SphI Sse8387I
StuI StyI TaqI TseI Tsp45I
TspRI TthHB8I Van91I XcmI XmaCI
XmaI