##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I ApaLI AscI Asp718I
AspEI AviII BbrPI BfrI BglII
BlpI Bpu1102I BsiWI BssHII BstBI
BstEII BstPI CelII CpoI Eam1105I
EcoO65I EcoRI FspI KpnI KspI
MluI NheI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI Psp1406I RsrII SacII SalI
SbfI SfiI SfuI SmaI SnaBI
Sse8387I SwaI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccIII 4319 TCCGGA T^CCGGA
AcyI 100 GGCGCC G[GA]^CG[CT]C
Aor51HI 11 AGCGCT AGC^GCT
ApaI 6687 GGGCCC GGGCC^C
AvaI 341 CTCGAG C^[CT]CG[GA]G
AvrII 5212 CCTAGG C^CTAGG
BamHI 3670 GGATCC G^GATCC
BbeI 100 GGCGCC GGCGC^C
BbvCI 4489 CCTCAGC CCTCAGC(-5/-2)
BglI 3250 GCCGCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 5212 CCTAGG C^CTAGG
BsaAI 6735 CACGTA [CT]AC^GT[GA]
BsaBI 3052 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 100 GGCGCC G[GA]^CG[CT]C
BsaI 1599 GGTCTC GGTCTC(1/5)
BsaWI 4319 TCCGGA [AT]^CCGG[AT]
BseAI 4319 TCCGGA T^CCGGA
BsmBI 2073 GAGACG CGTCTC(1/5)
BsoBI 341 CTCGAG C^[CT]CG[GA]G
BspDI 3227 ATCGAT AT^CGAT
BspEI 4319 TCCGGA T^CCGGA
BspHI 5262 TCATGA T^CATGA
Bst1107I 6560 GTATAC GTA^TAC
BstZ17I 6560 GTATAC GTA^TAC
ClaI 3227 ATCGAT AT^CGAT
DrdI 296 GACAAATATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 11 AGCGCT AGC^GCT
EcoNI 2958 CCTTTCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 4772 GGCCGGCC GGCCGG^CC
Hin1I 100 GGCGCC G[GA]^CG[CT]C
HpaI 6858 GTTAAC GTT^AAC
KasI 100 GGCGCC G^GCGCC
MamI 3052 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 7224 CAATTG C^AATTG
MroI 4319 TCCGGA T^CCGGA
MunI 7224 CAATTG C^AATTG
NaeI 4773 GCCGGC GCC^GGC
NarI 100 GGCGCC GG^CGCC
NgoMIV 4773 GCCGGC G^CCGGC
PaeR7I 341 CTCGAG C^TCGAG
PvuI 272 CGATCG CGAT^CG
RcaI 5262 TCATGA T^CATGA
SacI 4286 GAGCTC GAGCT^C
SapI 3442 GAAGAGC GCTCTTC(1/4)
ScaI 2616 AGTACT AGT^ACT
SfoI 100 GGCGCC GGC^GCC
SgrAI 106 CACCGGCG C[GA]^CCGG[CT]G
SpeI 3661 ACTAGT A^CTAGT
XhoI 341 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 6524 GTAGAC GT^[AC][GT]AC
6560 GTATAC
AocI 3413 CCTCAGG CC^T[ACGT]AGG
3643 CCTGAGG
Asp700I 2008 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5886 GAATTGTTTC
BcgI 3852 CGAACTTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3916 GCAGCTGACTCG
BciVI 771 GGATAC GTATCC(6/5)
1941 GGATAC
BsmI 2648 GAATGC GAATGC(1/-1)
6850 GCATTC
BsrFI 107 ACCGGC [GA]^CCGG[CT]
4773 GCCGGC
BstAPI 4047 GCAGAATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5760 GCAAACATTGC
BstXI 5580 CCATATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6713 CCACAAGCGTGG
Bsu36I 3413 CCTCAGG CC^T[ACGT]AGG
3643 CCTGAGG
Cfr10I 107 ACCGGC [GA]^CCGG[CT]
4773 GCCGGC
DraIII 1550 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
5069 CACTGGGTG
EagI 4771 CGGCCG C^GGCCG
4775 CGGCCG
EclXI 4771 CGGCCG C^GGCCG
4775 CGGCCG
Eco52I 4771 CGGCCG C^GGCCG
4775 CGGCCG
Eco81I 3413 CCTCAGG CC^T[ACGT]AGG
3643 CCTGAGG
EcoT22I 1827 ATGCAT ATGCA^T
6302 ATGCAT
HgaI 2785 GCGTC GACGC(5/10)
4537 GCGTC
HindIII 389 AAGCTT A^AGCTT
5799 AAGCTT
NcoI 4293 CCATGG C^CATGG
4344 CCATGG
NsiI 1827 ATGCAT ATGCA^T
6302 ATGCAT
PpuMI 927 AGGTCCT [GA]G^G[AT]CC[CT]
2922 GGGTCCT
SmlI 341 CTCGAG C^T[CT][GA]AG
4561 CTCAAG
XmnI 2008 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5886 GAATTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI AspHI AspI AvaII BalI
BanI BanII BbsI BbvI BclI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspLU11I BspMI BsrBI BsrDI
BsrGI BsrI BssKI BssSI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoRV EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PshBI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SphI SspBI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII