##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AvrII BciVI BfrI BlnI
BsaBI BsiWI Bsp1407I BspDI BspHI
BsrGI BssHII BstBI ClaI Eco47III
EcoRV FseI HpaI KpnI MamI
MfeI MluI MunI NdeI NheI
NotI NruI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RcaI
SalI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccI 535 GTATAC GT^[AC][GT]AC
AhdI 1354 GACGGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1354 GACGGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3436 TGCGCA TGC^GCA
BamHI 5770 GGATCC G^GATCC
BbeI 4988 GGCGCC GGCGC^C
BbvCI 2898 GCTGAGG CCTCAGC(-5/-2)
BclI 4524 TGATCA T^GATCA
BspLU11I 6143 ACATGT A^CATGT
BsrDI 3694 CATTGC GCAATG(2/0)
Bst1107I 535 GTATAC GTA^TAC
BstAPI 3984 GCATCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 535 GTATAC GTA^TAC
CpoI 6170 CGGACCG CG^G[AT]CCG
Eam1105I 1354 GACGGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2795 GAATTC G^AATTC
EcoT22I 6153 ATGCAT ATGCA^T
FbaI 4524 TGATCA T^GATCA
FspI 3436 TGCGCA TGC^GCA
HindIII 3480 AAGCTT A^AGCTT
KasI 4988 GGCGCC G^GCGCC
KspI 3549 CCGCGG CCGC^GG
NarI 4988 GGCGCC GG^CGCC
NsiI 6153 ATGCAT ATGCA^T
RsrII 6170 CGGACCG CG^G[AT]CCG
SacII 3549 CCGCGG CCGC^GG
SapI 2009 GCTCTTC GCTCTTC(1/4)
ScaI 2594 AGTACT AGT^ACT
SexAI 5186 ACCTGGT A^CC[AT]GGT
SfiI 3657 GGCCATGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 4988 GGCGCC GGC^GCC
XbaI 3511 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcsI 220 AAATTC [GA]^AATT[CT]
2795 GAATTC
Alw44I 6005 GTGCAC G^TGCAC
6163 GTGCAC
ApaLI 6005 GTGCAC G^TGCAC
6163 GTGCAC
ApoI 220 AAATTC [GA]^AATT[CT]
2795 GAATTC
BbrPI 745 CACGTG CAC^GTG
4684 CACGTG
BcgI 465 CGACGCCGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3202 CGAGGCCCATGC
BglII 848 AGATCT A^GATCT
4026 AGATCT
BlpI 2256 GCTCAGC GC^T[ACGT]AGC
4658 GCTGAGC
Bpu1102I 2256 GCTCAGC GC^T[ACGT]AGC
4658 GCTGAGC
BsiEI 1998 CGGCCG CG[GA][CT]^CG
5643 CGGCCG
BsmBI 2950 GAGACG CGTCTC(1/5)
4290 GAGACG
BstEII 244 GGTTACC G^GT[ACGT]ACC
5756 GGTGACC
BstPI 244 GGTTACC G^GT[ACGT]ACC
5756 GGTGACC
BstXI 2516 CCATGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4279 CCACCTCCATGG
CelII 2256 GCTCAGC GC^T[ACGT]AGC
4658 GCTGAGC
DraIII 1529 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
4333 CACTTGGTG
DrdI 2576 GACTCACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5946 GACAGCACGGTC
EagI 1998 CGGCCG C^GGCCG
5643 CGGCCG
EclXI 1998 CGGCCG C^GGCCG
5643 CGGCCG
Eco52I 1998 CGGCCG C^GGCCG
5643 CGGCCG
EcoO65I 244 GGTTACC G^GT[ACGT]ACC
5756 GGTGACC
HincII 3367 GTTGAC GT[CT]^[GA]AC
3880 GTTGAC
HindII 3367 GTTGAC GT[CT]^[GA]AC
3880 GTTGAC
NaeI 1040 GCCGGC GCC^GGC
4463 GCCGGC
NgoMIV 1040 GCCGGC G^CCGGC
4463 GCCGGC
PaeR7I 1267 CTCGAG C^TCGAG
2387 CTCGAG
PmaCI 745 CACGTG CAC^GTG
4684 CACGTG
PmlI 745 CACGTG CAC^GTG
4684 CACGTG
PshAI 1791 GACGCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3370 GACCCCTGTC
SacI 1905 GAGCTC GAGCT^C
5914 GAGCTC
SphI 4478 GCATGC GCATG^C
5397 GCATGC
StuI 2182 AGGCCT AGG^CCT
4750 AGGCCT
XcmI 2496 CCACCGTGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5177 CCAGGGAGGACCTGG
XhoI 1267 CTCGAG C^TCGAG
2387 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI AspHI AspI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspEI BspMI BsrBI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MroI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I SbfI ScrFI SfaNI
SfcI SmaI SmlI Sse8387I StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XhoII XmaCI XmaI