##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AccI AclI AflII AflIII AgeI
Alw44I ApaLI AscI AseI AsnI
AvrII BamHI BciVI BfrI BlnI
BsaBI BsiWI BspDI BspHI BspLU11I
BssSI Bst1107I BstZ17I ClaI CpoI
DrdI EagI EclXI Eco52I EcoRI
EcoRV EcoT22I HincII HindII HindIII
HpaI KspI MamI MfeI MluI
MunI NotI NsiI PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SgrAI SnaBI
SphI Sse8387I SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 5229 GGTACC G^GTACC
AccIII 414 TCCGGA T^CCGGA
Aor51HI 5731 AGCGCT AGC^GCT
Asp700I 751 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 5229 GGTACC G^GTACC
BclI 5696 TGATCA T^GATCA
BseAI 414 TCCGGA T^CCGGA
BsmI 2533 GCATTC GAATGC(1/-1)
BspEI 414 TCCGGA T^CCGGA
BssHII 954 GCGCGC G^CGCGC
BstAPI 889 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3261 GGTCACC G^GT[ACGT]ACC
BstPI 3261 GGTCACC G^GT[ACGT]ACC
DraIII 629 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 5731 AGCGCT AGC^GCT
EcoO65I 3261 GGTCACC G^GT[ACGT]ACC
FbaI 5696 TGATCA T^GATCA
KpnI 5229 GGTACC GGTAC^C
MroI 414 TCCGGA T^CCGGA
NdeI 6110 CATATG CA^TATG
NheI 4766 GCTAGC G^CTAGC
NruI 2803 TCGCGA TCG^CGA
SapI 3475 GCTCTTC GCTCTTC(1/4)
SpeI 1780 ACTAGT A^CTAGT
XmnI 751 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 1289 AAATTC [GA]^AATT[CT]
1434 GAATTT
AhdI 214 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3254 GACCTCCGGTC
ApoI 1289 AAATTC [GA]^AATT[CT]
1434 GAATTT
AspEI 214 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3254 GACCTCCGGTC
AspI 2207 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3385 GACGCTGTC
AviII 1842 TGCGCA TGC^GCA
4062 TGCGCA
BbrPI 1700 CACGTG CAC^GTG
4641 CACGTG
BcgI 4537 CGAGGCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
54 GCAATGAGATCG
BlpI 34 GCTCAGC GC^T[ACGT]AGC
1349 GCTGAGC
Bpu1102I 34 GCTCAGC GC^T[ACGT]AGC
1349 GCTGAGC
BsaWI 414 TCCGGA [AT]^CCGG[AT]
3258 TCCGGT
BsiEI 2614 CGACCG CG[GA][CT]^CG
2891 CGACCG
Bsp1407I 681 TGTACA T^GTACA
5973 TGTACA
BsrDI 54 GCAATG GCAATG(2/0)
1995 GCAATG
BsrGI 681 TGTACA T^GTACA
5973 TGTACA
BstBI 4427 TTCGAA TT^CGAA
5947 TTCGAA
CelII 34 GCTCAGC GC^T[ACGT]AGC
1349 GCTGAGC
DraI 4723 TTTAAA TTT^AAA
5273 TTTAAA
Eam1105I 214 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3254 GACCTCCGGTC
FspI 1842 TGCGCA TGC^GCA
4062 TGCGCA
NspV 4427 TTCGAA TT^CGAA
5947 TTCGAA
PaeR7I 2980 CTCGAG C^TCGAG
3704 CTCGAG
PflFI 2207 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3385 GACGCTGTC
PmaCI 1700 CACGTG CAC^GTG
4641 CACGTG
PmlI 1700 CACGTG CAC^GTG
4641 CACGTG
SfuI 4427 TTCGAA TT^CGAA
5947 TTCGAA
SspBI 681 TGTACA T^GTACA
5973 TGTACA
StuI 2156 AGGCCT AGG^CCT
4318 AGGCCT
Tth111I 2207 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3385 GACGCTGTC
XhoI 2980 CTCGAG C^TCGAG
3704 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BglI BglII BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FseI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI