##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
Aor51HI ApaI AscI AseI AsnI
Asp718I AviII BbeI BbrPI BcgI
BglI BsaBI BsaHI BseAI BsiWI
BsmBI BspEI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Cfr10I DrdI Eco47III EcoO65I
EcoRV FseI FspI Hin1I KasI
KpnI MamI MroI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI PstI PvuI
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SphI Sse8387I SwaI
XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AclI 6386 AACGTT AA^CGTT
AhdI 3858 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4696 GTGCAC G^TGCAC
ApaLI 4696 GTGCAC G^TGCAC
AspEI 3858 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2250 CCTAGG C^CTAGG
BanI 4420 GGTGCC G^G[CT][GA]CC
BbvCI 455 GCTGAGG CCTCAGC(-5/-2)
BclI 6982 TGATCA T^GATCA
BlnI 2250 CCTAGG C^CTAGG
BlpI 2442 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2442 GCTGAGC GC^T[ACGT]AGC
CelII 2442 GCTGAGC GC^T[ACGT]AGC
CpoI 27 CGGTCCG CG^G[AT]CCG
DraIII 6869 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 597 CGGCCG C^GGCCG
Eam1105I 3858 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 597 CGGCCG C^GGCCG
Eco52I 597 CGGCCG C^GGCCG
FbaI 6982 TGATCA T^GATCA
HaeII 195 GGCGCT [GA]GCGC^[CT]
HgaI 483 GCGTC GACGC(5/10)
KspI 366 CCGCGG CCGC^GG
MluI 481 ACGCGT A^CGCGT
Psp1406I 6386 AACGTT AA^CGTT
RsrII 27 CGGTCCG CG^G[AT]CCG
SacII 366 CCGCGG CCGC^GG
SmaI 391 CCCGGG CCC^GGG
XmaCI 391 CCCGGG C^CCGGG
XmaI 391 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 4133 GTCTAC GT^[AC][GT]AC
6164 GTAGAC
AflII 3381 CTTAAG C^TTAAG
7369 CTTAAG
AspI 116 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2281 GACCCCGTC
AvaI 391 CCCGGG C^[CT]CG[GA]G
2962 CTCGGG
BbsI 2606 GAAGAC GAAGAC(2/6)
2287 GTCTTC
BfrI 3381 CTTAAG C^TTAAG
7369 CTTAAG
BglII 1637 AGATCT A^GATCT
3342 AGATCT
BpuAI 2606 GAAGAC GAAGAC(2/6)
2287 GTCTTC
BsaAI 4563 TACGTA [CT]AC^GT[GA]
7113 TACGTA
BsaI 541 GGTCTC GGTCTC(1/5)
823 GGTCTC
BsiEI 214 CGACCG CG[GA][CT]^CG
597 CGGCCG
BsoBI 391 CCCGGG C^[CT]CG[GA]G
2962 CTCGGG
BspDI 1666 ATCGAT AT^CGAT
4771 ATCGAT
BspHI 1672 TCATGA T^CATGA
3251 TCATGA
BspMI 1382 GCAGGT ACCTGC(4/8)
2681 GCAGGT
BsrBI 489 GAGCGG CCGCTC(-3/-3)
612 GAGCGG
CfoI 196 GCGC GCG^C
4380 GCGC
ClaI 1666 ATCGAT AT^CGAT
4771 ATCGAT
EcoNI 732 CCTCTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
6946 CCTGGCTAAGG
EcoRI 4809 GAATTC G^AATTC
5622 GAATTC
HhaI 196 GCGC GCG^C
4380 GCGC
HinP1I 196 GCGC G^CGC
4380 GCGC
MfeI 149 CAATTG C^AATTG
5377 CAATTG
MunI 149 CAATTG C^AATTG
5377 CAATTG
NcoI 4893 CCATGG C^CATGG
7388 CCATGG
NdeI 5232 CATATG CA^TATG
5509 CATATG
PflFI 116 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2281 GACCCCGTC
PflMI 5045 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5117 CCAGTTGCTGG
PpuMI 506 GGGACCC [GA]G^G[AT]CC[CT]
2279 AGGACCC
RcaI 1672 TCATGA T^CATGA
3251 TCATGA
SnaBI 4563 TACGTA TAC^GTA
7113 TACGTA
SpeI 6327 ACTAGT A^CTAGT
6780 ACTAGT
Tth111I 116 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2281 GACCCCGTC
Van91I 5045 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5117 CCAGTTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaII BalI BamHI
BanII BbvI BciVI BcnI BfaI
BmyI BpmI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspLU11I
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeIII HapII HincII
HindII HindIII HinfI HpaI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PleI PvuII RsaI SacI SapI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SspBI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI