##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AclI AgeI AhdI Alw44I
AocI Aor51HI ApaLI Asp700I AspEI
AviII BbrPI BlpI Bpu1102I BsiEI
BsiWI BspDI BspHI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I FseI FspI HindIII MluI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SexAI SfiI SfuI SnaBI
SphI Sse8387I SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccIII 386 TCCGGA T^CCGGA
AflII 2084 CTTAAG C^TTAAG
AflIII 3170 ACATGT A^C[GA][CT]GT
ApaI 44 GGGCCC GGGCC^C
AvrII 3105 CCTAGG C^CTAGG
BamHI 1609 GGATCC G^GATCC
BcgI 737 GCAACATTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2084 CTTAAG C^TTAAG
BglI 1249 GCCAAATAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 292 AGATCT A^GATCT
BlnI 3105 CCTAGG C^CTAGG
BsaBI 3920 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 386 TCCGGA [AT]^CCGG[AT]
BseAI 386 TCCGGA T^CCGGA
Bsp1407I 4593 TGTACA T^GTACA
BspEI 386 TCCGGA T^CCGGA
BspLU11I 3170 ACATGT A^CATGT
BspMI 5261 ACCTGC ACCTGC(4/8)
BsrBI 189 CCGCTC CCGCTC(-3/-3)
BsrDI 4783 GCAATG GCAATG(2/0)
BsrFI 892 GCCGGC [GA]^CCGG[CT]
BsrGI 4593 TGTACA T^GTACA
BstAPI 233 GCACTGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 892 GCCGGC [GA]^CCGG[CT]
EcoNI 596 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 3330 GATATC GAT^ATC
EcoT22I 3497 ATGCAT ATGCA^T
HpaI 443 GTTAAC GTT^AAC
MamI 3920 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4690 CAATTG C^AATTG
MroI 386 TCCGGA T^CCGGA
MunI 4690 CAATTG C^AATTG
NaeI 892 GCCGGC GCC^GGC
NdeI 1936 CATATG CA^TATG
NgoMIV 892 GCCGGC G^CCGGC
NheI 5174 GCTAGC G^CTAGC
NsiI 3497 ATGCAT ATGCA^T
PaeR7I 621 CTCGAG C^TCGAG
PflMI 536 CCAGCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 364 CTGCAG CTGCA^G
SapI 1989 GAAGAGC GCTCTTC(1/4)
SgrAI 891 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 1971 ACTAGT A^CTAGT
SspBI 4593 TGTACA T^GTACA
Van91I 536 CCAGCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 586 CCACTCTGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 621 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AscI 176 GGCGCGCC GG^CGCGCC
264 GGCGCGCC
BbeI 558 GGCGCC GGCGC^C
805 GGCGCC
BciVI 3975 GGATAC GTATCC(6/5)
1975 GTATCC
BclI 1888 TGATCA T^GATCA
4243 TGATCA
BsaAI 135 TACGTG [CT]AC^GT[GA]
5289 CACGTA
DraIII 234 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
1466 CACTTTGTG
EcoRI 858 GAATTC G^AATTC
4619 GAATTC
FbaI 1888 TGATCA T^GATCA
4243 TGATCA
KasI 558 GGCGCC G^GCGCC
805 GGCGCC
NarI 558 GGCGCC GG^CGCC
805 GGCGCC
NcoI 40 CCATGG C^CATGG
1944 CCATGG
NspI 3170 ACATGT [GA]CATG^[CT]
4360 ACATGC
SacI 1910 GAGCTC GAGCT^C
2856 GAGCTC
SfoI 558 GGCGCC GGC^GCC
805 GGCGCC
SmaI 358 CCCGGG CCC^GGG
3779 CCCGGG
XmaCI 358 CCCGGG C^CCGGG
3779 CCCGGG
XmaI 358 CCCGGG C^CCGGG
3779 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AcyI AfaI AluI AlwI AlwNI
ApoI AseI AsnI Asp718I AspHI
AspI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BsrI BssHII
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KpnI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflFI PleI
PpuMI PshBI PvuII RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XhoII