##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI ApaI AscI Asp718I AspEI
AviII BamHI BbrPI BbvCI BciVI
BlpI Bpu1102I BsaAI BsaBI BseAI
BsiEI BsiWI BspEI BsrBI BsrFI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I CelII Cfr10I CpoI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRI
FseI FspI HincII HindII HpaI
KpnI KspI MamI MroI NaeI
NgoMIV NheI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SfiI SfuI SgrAI
SmaI SnaBI Sse8387I SspI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccI 1735 GTAGAC GT^[AC][GT]AC
AflII 4855 CTTAAG C^TTAAG
Aor51HI 4119 AGCGCT AGC^GCT
AspI 2399 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 1013 GCAACTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 4554 CCCGG CC^[GC]GG
BfrI 4855 CTTAAG C^TTAAG
BsaI 339 GAGACC GGTCTC(1/5)
BsaWI 1318 ACCGGA [AT]^CCGG[AT]
BsmBI 415 CGTCTC CGTCTC(1/5)
BsmI 2219 GCATTC GAATGC(1/-1)
BspDI 502 ATCGAT AT^CGAT
BssSI 1387 CACGAG CACGAG(-5/-1)
ClaI 502 ATCGAT AT^CGAT
DraIII 2150 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4068 GACTCATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4119 AGCGCT AGC^GCT
EcoRV 3954 GATATC GAT^ATC
MfeI 2181 CAATTG C^AATTG
MluI 412 ACGCGT A^CGCGT
MunI 2181 CAATTG C^AATTG
NciI 4554 CCCGG CC^[GC]GG
NcoI 3316 CCATGG C^CATGG
NdeI 3945 CATATG CA^TATG
PflFI 2399 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1738 GACATTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 712 CAGCTG CAG^CTG
SacI 3283 GAGCTC GAGCT^C
ScaI 4600 AGTACT AGT^ACT
SexAI 904 ACCTGGT A^CC[AT]GGT
SphI 5028 GCATGC GCATG^C
SwaI 2240 ATTTAAAT ATTT^AAAT
Tth111I 2399 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 413 CGCG CG^CG
3759 CGCG
Alw44I 4083 GTGCAC G^TGCAC
5243 GTGCAC
ApaLI 4083 GTGCAC G^TGCAC
5243 GTGCAC
AseI 1906 ATTAAT AT^TAAT
2034 ATTAAT
AsnI 1906 ATTAAT AT^TAAT
2034 ATTAAT
Asp700I 4246 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5284 GAACTAATTC
AvaI 1198 CTCGAG C^[CT]CG[GA]G
3010 CTCGAG
AvrII 2442 CCTAGG C^CTAGG
3442 CCTAGG
BbeI 2413 GGCGCC GGCGC^C
3320 GGCGCC
BbsI 1464 GAAGAC GAAGAC(2/6)
4579 GAAGAC
BclI 1502 TGATCA T^GATCA
1928 TGATCA
BglI 1204 GCCTGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4997 GCCAGGACGGC
BlnI 2442 CCTAGG C^CTAGG
3442 CCTAGG
BpuAI 1464 GAAGAC GAAGAC(2/6)
4579 GAAGAC
BsoBI 1198 CTCGAG C^[CT]CG[GA]G
3010 CTCGAG
BspLU11I 531 ACATGT A^CATGT
652 ACATGT
BstUI 413 CGCG CG^CG
3759 CGCG
EcoNI 519 CCTCTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2654 CCTGGAGAAGG
FbaI 1502 TGATCA T^GATCA
1928 TGATCA
KasI 2413 GGCGCC G^GCGCC
3320 GGCGCC
MaeII 1004 ACGT A^CGT
3255 ACGT
MvnI 413 CGCG CG^CG
3759 CGCG
NarI 2413 GGCGCC GG^CGCC
3320 GGCGCC
PaeR7I 1198 CTCGAG C^TCGAG
3010 CTCGAG
PpuMI 1347 GGGACCT [GA]G^G[AT]CC[CT]
2522 GGGACCC
PshBI 1906 ATTAAT AT^TAAT
2034 ATTAAT
SfoI 2413 GGCGCC GGC^GCC
3320 GGCGCC
SpeI 1779 ACTAGT A^CTAGT
5263 ACTAGT
XhoI 1198 CTCGAG C^TCGAG
3010 CTCGAG
XmnI 4246 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5284 GAACTAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaII BalI BanI BanII
BbvI BfaI BglII BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspHI BspMI BsrDI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PstI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XcmI XhoII