##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI Aor51HI ApaI AscI Asp718I
AviII AvrII BamHI BbeI BbrPI
BcgI BglI BlnI BsaAI BsaBI
BsaHI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BsrDI
BsrFI BsrGI BssHII BssSI BstBI
BstUI Cfr10I ClaI CpoI DraIII
DrdI DsaI EagI EclXI Eco47III
Eco52I EcoRV FseI FspI HaeII
Hin1I KasI KpnI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PstI PvuI RcaI RsrII SacII
SapI SbfI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AclI 3552 AACGTT AA^CGTT
AflII 3785 CTTAAG C^TTAAG
AhdI 428 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 660 GTGCAC G^TGCAC
ApaLI 660 GTGCAC G^TGCAC
AspEI 428 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4662 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 1216 GGTGCC G^G[CT][GA]CC
BbsI 1331 GTCTTC GAAGAC(2/6)
BciVI 1930 GTATCC GTATCC(6/5)
BfrI 3785 CTTAAG C^TTAAG
BlpI 2757 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2757 GCTGAGC GC^T[ACGT]AGC
BpuAI 1331 GTCTTC GAAGAC(2/6)
BsiEI 4571 CGGTCG CG[GA][CT]^CG
BsrBI 4568 GAGCGG CCGCTC(-3/-3)
BstAPI 2762 GCAAAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 723 GGTTACC G^GT[ACGT]ACC
BstPI 723 GGTTACC G^GT[ACGT]ACC
CelII 2757 GCTGAGC GC^T[ACGT]AGC
CfoI 2411 GCGC GCG^C
DraII 87 AGGACCC [GA]G^G[ACGT]CC[CT]
Eam1105I 428 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 87 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 723 GGTTACC G^GT[ACGT]ACC
EcoRI 795 GAATTC G^AATTC
HgaI 138 GCGTC GACGC(5/10)
HhaI 2411 GCGC GCG^C
HinP1I 2411 GCGC G^CGC
HpaI 1420 GTTAAC GTT^AAC
MslI 786 CACGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I 1046 CTCGAG C^TCGAG
PflFI 4662 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 87 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 3552 AACGTT AA^CGTT
PvuII 1085 CAGCTG CAG^CTG
SalI 140 GTCGAC G^TCGAC
SexAI 429 ACCAGGT A^CC[AT]GGT
SphI 4515 GCATGC GCATG^C
StuI 2029 AGGCCT AGG^CCT
SwaI 1283 ATTTAAAT ATTT^AAAT
Tth111I 4662 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1101 CCAGGAGATGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1046 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AflIII 1472 ACATGT A^C[GA][CT]GT
2439 ACATGT
AlwNI 199 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1268 CAGATTCTG
AseI 2082 ATTAAT AT^TAAT
3651 ATTAAT
AsnI 2082 ATTAAT AT^TAAT
3651 ATTAAT
BalI 887 TGGCCA TGG^CCA
1146 TGGCCA
BbvCI 294 CCTCAGC CCTCAGC(-5/-2)
3238 GCTGAGG
BclI 3664 TGATCA T^GATCA
3719 TGATCA
BglII 2735 AGATCT A^GATCT
3603 AGATCT
BpmI 955 CTCCAG CTGGAG(16/14)
1099 CTCCAG
BsaI 1119 GAGACC GGTCTC(1/5)
3204 GGTCTC
BseRI 49 GAGGAG GAGGAG(10/8)
508 GAGGAG
BsgI 267 CTGCAC GTGCAG(16/14)
4552 GTGCAG
BsmFI 2866 GGGAC GGGAC(10/14)
3302 GTCCC
BsmI 3108 GAATGC GAATGC(1/-1)
2695 GCATTC
BspLU11I 1472 ACATGT A^CATGT
2439 ACATGT
BspMI 610 GCAGGT ACCTGC(4/8)
3278 GCAGGT
Bst1107I 3636 GTATAC GTA^TAC
3792 GTATAC
BstXI 878 CCATGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1221 CCAAGAGAATGG
BstZ17I 3636 GTATAC GTA^TAC
3792 GTATAC
EaeI 887 TGGCCA [CT]^GGCC[GA]
1146 TGGCCA
EcoT22I 2416 ATGCAT ATGCA^T
4513 ATGCAT
FauI 3063 CCCGC CCCGC(4/6)
3353 CCCGC
FbaI 3664 TGATCA T^GATCA
3719 TGATCA
HindIII 2612 AAGCTT A^AGCTT
4392 AAGCTT
MaeII 2786 ACGT A^CGT
3553 ACGT
MluNI 887 TGGCCA TGG^CCA
1146 TGGCCA
MscI 887 TGGCCA TGG^CCA
1146 TGGCCA
MspA1I 187 CAGCGG C[AC]G^C[GT]G
1085 CAGCTG
NsiI 2416 ATGCAT ATGCA^T
4513 ATGCAT
PshBI 2082 ATTAAT AT^TAAT
3651 ATTAAT
SacI 63 GAGCTC GAGCT^C
4152 GAGCTC
ScaI 4384 AGTACT AGT^ACT
4430 AGTACT
SmaI 463 CCCGGG CCC^GGG
2496 CCCGGG
SspI 696 AATATT AAT^ATT
2219 AATATT
XmaCI 463 CCCGGG C^CCGGG
2496 CCCGGG
XmaI 463 CCCGGG C^CCGGG
2496 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AocI ApoI Asp700I AspHI
AvaI AvaII BanII BbvI BcnI
BfaI BmyI BsaJI BsiHKAI BsiYI
BslI BsmAI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII DraI
EarI Eco81I EcoNI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XhoII XmnI