##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AhdI AscI AseI AsnI Asp718I
AspEI AviII AvrII BbeI BbrPI
BciVI BclI BglI BlnI BlpI
Bpu1102I BseAI BsiEI BsiWI BspDI
BspEI BssHII Bst1107I BstZ17I CelII
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco52I FbaI FseI FspI
HpaI KasI KpnI KspI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NheI NotI NruI PacI
PaeR7I PflMI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SnaBI Sse8387I
SwaI Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccII 599 CGCG CG^CG
AcyI 1898 GACGCC G[GA]^CG[CT]C
AgeI 1875 ACCGGT A^CCGGT
Alw44I 5019 GTGCAC G^TGCAC
AlwNI 182 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 310 CCTCAGG CC^T[ACGT]AGG
Aor51HI 628 AGCGCT AGC^GCT
ApaI 5375 GGGCCC GGGCC^C
ApaLI 5019 GTGCAC G^TGCAC
BalI 2124 TGGCCA TGG^CCA
BcgI 8 GCATCCGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 552 TACGTG [CT]AC^GT[GA]
BsaBI 4674 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1898 GACGCC G[GA]^CG[CT]C
BsaWI 1875 ACCGGT [AT]^CCGG[AT]
BsgI 3023 GTGCAG GTGCAG(16/14)
BsmBI 1957 GAGACG CGTCTC(1/5)
BspHI 5721 TCATGA T^CATGA
BsrBI 842 GAGCGG CCGCTC(-3/-3)
BsrFI 1875 ACCGGT [GA]^CCGG[CT]
BstBI 1929 TTCGAA TT^CGAA
BstEII 5275 GGTGACC G^GT[ACGT]ACC
BstPI 5275 GGTGACC G^GT[ACGT]ACC
BstUI 599 CGCG CG^CG
BstXI 5854 CCATGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 310 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1875 ACCGGT [GA]^CCGG[CT]
Eco47III 628 AGCGCT AGC^GCT
Eco81I 310 CCTCAGG CC^T[ACGT]AGG
EcoO65I 5275 GGTGACC G^GT[ACGT]ACC
EcoRV 2081 GATATC GAT^ATC
Hin1I 1898 GACGCC G[GA]^CG[CT]C
HincII 4468 GTTGAC GT[CT]^[GA]AC
HindII 4468 GTTGAC GT[CT]^[GA]AC
MamI 4674 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2124 TGGCCA TGG^CCA
MscI 2124 TGGCCA TGG^CCA
MspA1I 1644 CAGCTG C[AC]G^C[GT]G
MvnI 599 CGCG CG^CG
NspV 1929 TTCGAA TT^CGAA
PinAI 1875 ACCGGT A^CCGGT
PvuII 1644 CAGCTG CAG^CTG
RcaI 5721 TCATGA T^CATGA
SfuI 1929 TTCGAA TT^CGAA
SmaI 645 CCCGGG CCC^GGG
SphI 4707 GCATGC GCATG^C
StuI 1517 AGGCCT AGG^CCT
XmaCI 645 CCCGGG C^CCGGG
XmaI 645 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflII 683 CTTAAG C^TTAAG
5652 CTTAAG
AspI 14 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3900 GACAAGGTC
BamHI 2169 GGATCC G^GATCC
5850 GGATCC
BanI 2642 GGTGCC G^G[CT][GA]CC
5258 GGTGCC
BfrI 683 CTTAAG C^TTAAG
5652 CTTAAG
BglII 2019 AGATCT A^GATCT
5656 AGATCT
BspLU11I 2876 ACATGT A^CATGT
3419 ACATGT
BsrDI 1198 GCAATG GCAATG(2/0)
2939 GCAATG
BssSI 521 CACGAG CACGAG(-5/-1)
3145 CACGAG
DraIII 431 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
4414 CACAATGTG
EaeI 717 CGGCCA [CT]^GGCC[GA]
2124 TGGCCA
EcoT22I 4096 ATGCAT ATGCA^T
4705 ATGCAT
FauI 844 GCGGG CCCGC(4/6)
1183 GCGGG
HaeII 628 AGCGCT [GA]GCGC^[CT]
678 AGCGCC
HgaI 1745 GACGC GACGC(5/10)
1898 GACGC
HindIII 371 AAGCTT A^AGCTT
4539 AAGCTT
MaeII 553 ACGT A^CGT
1177 ACGT
NsiI 4096 ATGCAT ATGCA^T
4705 ATGCAT
PflFI 14 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3900 GACAAGGTC
SacI 1448 GAGCTC GAGCT^C
2495 GAGCTC
SapI 2452 GAAGAGC GCTCTTC(1/4)
3161 GAAGAGC
SpeI 2947 ACTAGT A^CTAGT
3067 ACTAGT
Tth111I 14 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3900 GACAAGGTC
XcmI 720 CCAAGAAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3374 CCATAACAAAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AspHI AvaI
AvaII BanII BbsI BbvCI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspMI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoNI
EcoO109I EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI