##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AhdI Aor51HI AscI AspEI AvrII
BbeI BbrPI BbvCI BglI BlnI
BlpI Bpu1102I BsaAI BsaHI BseAI
BsiEI BsmBI BspDI BspEI BssHII
BssSI CelII ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I FauI
FseI Hin1I KasI KspI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 2155 GGTACC G^GTACC
AflII 31 CTTAAG C^TTAAG
Alw44I 2205 GTGCAC G^TGCAC
ApaI 2575 GGGCCC GGGCC^C
ApaLI 2205 GTGCAC G^TGCAC
Asp718I 2155 GGTACC G^GTACC
AspHI 2205 GTGCAC G[AT]GC[AT]^C
AspI 3251 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 121 TGCGCA TGC^GCA
BanI 2155 GGTACC G^G[CT][GA]CC
BcnI 20 CCCGG CC^[GC]GG
BfrI 31 CTTAAG C^TTAAG
BsaBI 989 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1842 ACCGGA [AT]^CCGG[AT]
BsiHKAI 2205 GTGCAC G[AT]GC[AT]^C
BsiWI 1807 CGTACG C^GTACG
BsmI 2547 GAATGC GAATGC(1/-1)
BsrBI 65 GAGCGG CCGCTC(-3/-3)
BsrFI 4510 ACCGGC [GA]^CCGG[CT]
Bst1107I 4202 GTATAC GTA^TAC
BstEII 3761 GGTTACC G^GT[ACGT]ACC
BstPI 3761 GGTTACC G^GT[ACGT]ACC
BstZ17I 4202 GTATAC GTA^TAC
Cfr10I 4510 ACCGGC [GA]^CCGG[CT]
DrdI 4593 GACATGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3761 GGTTACC G^GT[ACGT]ACC
EcoRI 3137 GAATTC G^AATTC
EcoRV 2174 GATATC GAT^ATC
EcoT22I 4217 ATGCAT ATGCA^T
FspI 121 TGCGCA TGC^GCA
HgaI 2740 GCGTC GACGC(5/10)
HindIII 3532 AAGCTT A^AGCTT
HpaI 4691 GTTAAC GTT^AAC
KpnI 2155 GGTACC GGTAC^C
MamI 989 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI 20 CCCGG CC^[GC]GG
NruI 3820 TCGCGA TCG^CGA
NsiI 4217 ATGCAT ATGCA^T
PflFI 3251 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 2901 CTGCAG CTGCA^G
SphI 1905 GCATGC GCATG^C
SspI 1418 AATATT AAT^ATT
StuI 372 AGGCCT AGG^CCT
Tth111I 3251 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 584 GTAGAC GT^[AC][GT]AC
4202 GTATAC
AvaI 1191 CTCGGG C^[CT]CG[GA]G
1707 CCCGAG
BamHI 730 GGATCC G^GATCC
1996 GGATCC
BcgI 2207 GCACATTATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3811 GCATCTTATTCG
BciVI 1387 GTATCC GTATCC(6/5)
4028 GTATCC
BclI 559 TGATCA T^GATCA
4798 TGATCA
BglII 3062 AGATCT A^GATCT
3176 AGATCT
BsoBI 1191 CTCGGG C^[CT]CG[GA]G
1707 CCCGAG
Bsp1407I 3744 TGTACA T^GTACA
4212 TGTACA
BspLU11I 3747 ACATGT A^CATGT
4209 ACATGT
BsrDI 563 CATTGC GCAATG(2/0)
2979 CATTGC
BsrGI 3744 TGTACA T^GTACA
4212 TGTACA
BstAPI 113 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3589 GCAGCTCTTGC
BstBI 2215 TTCGAA TT^CGAA
3159 TTCGAA
BstXI 82 CCACTGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3942 CCAGGGCAGTGG
DraIII 1241 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
4802 CACTGAGTG
DsaI 108 CCATGG C^C[GA][CT]GG
1589 CCATGG
EcoNI 2582 CCTGGTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4580 CCTTCTAAAGG
FbaI 559 TGATCA T^GATCA
4798 TGATCA
NcoI 108 CCATGG C^CATGG
1589 CCATGG
NspV 2215 TTCGAA TT^CGAA
3159 TTCGAA
PpuMI 2193 GGGACCC [GA]G^G[AT]CC[CT]
2470 GGGTCCT
PvuII 644 CAGCTG CAG^CTG
4381 CAGCTG
ScaI 1434 AGTACT AGT^ACT
3441 AGTACT
SfuI 2215 TTCGAA TT^CGAA
3159 TTCGAA
SspBI 3744 TGTACA T^GTACA
4212 TGTACA
XcmI 129 CCAAGAAGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4479 CCACCCCTACTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AseI AsnI Asp700I AvaII BalI
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BspMI BsrI BssKI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV NspI PflMI PleI PshBI
RcaI RsaI SapI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XhoII XmnI