##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AccIII AcyI AgeI Aor51HI
AscI AspI AviII BbeI BcgI
BsaHI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BspHI BspLU11I BsrBI
BssHII BssSI BstEII BstPI BstXI
ClaI CpoI EagI EclXI Eco47III
Eco52I EcoO65I FseI FspI HaeII
Hin1I HpaI KasI KspI MluI
MroI NarI NdeI NheI NotI
NruI PflFI PinAI PmeI PshAI
PvuI RcaI RsrII SacII SapI
SbfI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI Tth111I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 1359 GGTACC G^GTACC
AccII 46 CGCG CG^CG
AflII 5505 CTTAAG C^TTAAG
ApaI 2209 GGGCCC GGGCC^C
Asp718I 1359 GGTACC G^GTACC
AvrII 1340 CCTAGG C^CTAGG
BalI 4920 TGGCCA TGG^CCA
BamHI 548 GGATCC G^GATCC
BbvCI 1493 GCTGAGG CCTCAGC(-5/-2)
BfrI 5505 CTTAAG C^TTAAG
BglI 3349 GCCTGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1340 CCTAGG C^CTAGG
BlpI 215 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 215 GCTAAGC GC^T[ACGT]AGC
BsmBI 2220 CGTCTC CGTCTC(1/5)
Bsp1407I 5564 TGTACA T^GTACA
BsrGI 5564 TGTACA T^GTACA
Bst1107I 3038 GTATAC GTA^TAC
BstAPI 1245 GCAAATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1606 TTCGAA TT^CGAA
BstUI 46 CGCG CG^CG
BstZ17I 3038 GTATAC GTA^TAC
CelII 215 GCTAAGC GC^T[ACGT]AGC
DrdI 3151 GACTTGAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 4258 CCATGG C^C[GA][CT]GG
EcoT22I 3984 ATGCAT ATGCA^T
HindIII 1470 AAGCTT A^AGCTT
KpnI 1359 GGTACC GGTAC^C
MluNI 4920 TGGCCA TGG^CCA
MscI 4920 TGGCCA TGG^CCA
MvnI 46 CGCG CG^CG
NaeI 3808 GCCGGC GCC^GGC
NcoI 4258 CCATGG C^CATGG
NgoMIV 3808 GCCGGC G^CCGGC
NsiI 3984 ATGCAT ATGCA^T
NspV 1606 TTCGAA TT^CGAA
PacI 5586 TTAATTAA TTAAT^TAA
PaeR7I 1419 CTCGAG C^TCGAG
PstI 3162 CTGCAG CTGCA^G
SacI 1416 GAGCTC GAGCT^C
SalI 3053 GTCGAC G^TCGAC
SexAI 3400 ACCAGGT A^CC[AT]GGT
SfuI 1606 TTCGAA TT^CGAA
SphI 3986 GCATGC GCATG^C
SspBI 5564 TGTACA T^GTACA
StuI 3347 AGGCCT AGG^CCT
XbaI 2375 TCTAGA T^CTAGA
XhoI 1419 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 3038 GTATAC GT^[AC][GT]AC
3053 GTCGAC
AclI 1745 AACGTT AA^CGTT
5471 AACGTT
AflIII 1179 ACGTGT A^C[GA][CT]GT
4021 ACACGT
AhdI 1583 GACTGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1763 GACAATTGGTC
Alw44I 820 GTGCAC G^TGCAC
3561 GTGCAC
ApaLI 820 GTGCAC G^TGCAC
3561 GTGCAC
AspEI 1583 GACTGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1763 GACAATTGGTC
AvaI 1012 CCCGAG C^[CT]CG[GA]G
1419 CTCGAG
BclI 2621 TGATCA T^GATCA
4205 TGATCA
BcnI 161 CCGGG CC^[GC]GG
2207 CCGGG
BglII 2732 AGATCT A^GATCT
4294 AGATCT
BsoBI 1012 CCCGAG C^[CT]CG[GA]G
1419 CTCGAG
BspMI 997 ACCTGC ACCTGC(4/8)
4568 ACCTGC
BsrFI 3373 ACCGGC [GA]^CCGG[CT]
3808 GCCGGC
Cfr10I 3373 ACCGGC [GA]^CCGG[CT]
3808 GCCGGC
DraIII 159 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
2743 CACCCAGTG
Eam1105I 1583 GACTGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1763 GACAATTGGTC
FauI 2013 CCCGC CCCGC(4/6)
171 GCGGG
FbaI 2621 TGATCA T^GATCA
4205 TGATCA
HgaI 1234 GACGC GACGC(5/10)
3749 GCGTC
MfeI 1765 CAATTG C^AATTG
2852 CAATTG
MunI 1765 CAATTG C^AATTG
2852 CAATTG
NciI 161 CCGGG CC^[GC]GG
2207 CCGGG
PflMI 386 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3717 CCACAACTTGG
PpuMI 478 AGGTCCC [GA]G^G[AT]CC[CT]
3277 AGGACCC
Psp1406I 1745 AACGTT AA^CGTT
5471 AACGTT
ScaI 4078 AGTACT AGT^ACT
5100 AGTACT
Van91I 386 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3717 CCACAACTTGG
XcmI 1705 CCAGTGTGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4422 CCATGCAGTGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AseI AsnI
Asp700I AspHI AvaII BanI BanII
BbrPI BbsI BbvI BciVI BfaI
BmyI BpmI BpuAI BsaAI BsaBI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EaeI EarI Eco81I EcoNI EcoO109I
EcoRI EcoRII EcoRV EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PmaCI PmlI PshBI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI