##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AgeI AhdI Aor51HI
ApaI AscI Asp718I AspEI BamHI
BbrPI BbvCI BcgI BcnI BlpI
Bpu1102I BsiEI BsiWI Bsp1407I BspHI
BspMI BsrBI BsrGI BssHII BssSI
BstAPI BstEII BstPI CelII CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I FseI HpaI KpnI
KspI NciI NcoI NdeI NheI
NotI NruI PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PvuI RcaI RsrII SacII SalI
SbfI ScaI SmaI Sse8387I SspBI
StuI Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AclI 3522 AACGTT AA^CGTT
AlwNI 3611 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 606 CCTCAGG CC^T[ACGT]AGG
AviII 1975 TGCGCA TGC^GCA
AvrII 5197 CCTAGG C^CTAGG
BanI 27 GGCGCC G^G[CT][GA]CC
BbeI 27 GGCGCC GGCGC^C
BlnI 5197 CCTAGG C^CTAGG
BpmI 1029 CTCCAG CTGGAG(16/14)
BsgI 722 CTGCAC GTGCAG(16/14)
BspLU11I 852 ACATGT A^CATGT
BsrFI 47 GCCGGC [GA]^CCGG[CT]
BstXI 1892 CCAAAACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 606 CCTCAGG CC^T[ACGT]AGG
Cfr10I 47 GCCGGC [GA]^CCGG[CT]
DsaI 920 CCACGG C^C[GA][CT]GG
Eco81I 606 CCTCAGG CC^T[ACGT]AGG
EcoNI 3497 CCTAAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1975 TGCGCA TGC^GCA
HgaI 4805 GACGC GACGC(5/10)
KasI 27 GGCGCC G^GCGCC
MluI 2848 ACGCGT A^CGCGT
NaeI 47 GCCGGC GCC^GGC
NarI 27 GGCGCC GG^CGCC
NgoMIV 47 GCCGGC G^CCGGC
PpuMI 1054 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 3522 AACGTT AA^CGTT
SacI 4418 GAGCTC GAGCT^C
SexAI 1252 ACCAGGT A^CC[AT]GGT
SfiI 73 GGCCGCTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 27 GGCGCC GGC^GCC
SgrAI 46 CGCCGGCG C[GA]^CCGG[CT]G
SnaBI 264 TACGTA TAC^GTA
SpeI 959 ACTAGT A^CTAGT
SwaI 5854 ATTTAAAT ATTT^AAAT
XbaI 5524 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 49 enzymes) #
###################################
AccII 13 CGCG CG^CG
2849 CGCG
AccIII 616 TCCGGA T^CCGGA
2505 TCCGGA
AcyI 27 GGCGCC G[GA]^CG[CT]C
4805 GACGCC
AflII 833 CTTAAG C^TTAAG
4326 CTTAAG
Alw44I 5205 GTGCAC G^TGCAC
5729 GTGCAC
ApaLI 5205 GTGCAC G^TGCAC
5729 GTGCAC
AseI 1262 ATTAAT AT^TAAT
4771 ATTAAT
AsnI 1262 ATTAAT AT^TAAT
4771 ATTAAT
AspI 63 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1403 GACTTGGTC
AvaI 1089 CTCGGG C^[CT]CG[GA]G
2523 CCCGAG
BalI 320 TGGCCA TGG^CCA
3189 TGGCCA
BanII 6 GGGCTC G[GA]GC[CT]^C
4418 GAGCTC
BciVI 2326 GGATAC GTATCC(6/5)
2557 GGATAC
BfrI 833 CTTAAG C^TTAAG
4326 CTTAAG
BglI 19 GCCGTCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
74 GCCGCTGTGGC
BglII 1117 AGATCT A^GATCT
4714 AGATCT
BsaAI 264 TACGTA [CT]AC^GT[GA]
5775 TACGTG
BsaBI 2253 GATCCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3133 GATTAACATC
BsaHI 27 GGCGCC G[GA]^CG[CT]C
4805 GACGCC
BsaI 1193 GAGACC GGTCTC(1/5)
2078 GAGACC
BseAI 616 TCCGGA T^CCGGA
2505 TCCGGA
BsmBI 21 CGTCTC CGTCTC(1/5)
2318 CGTCTC
BsmFI 4391 GGGAC GGGAC(10/14)
4787 GTCCC
BsmI 2672 GAATGC GAATGC(1/-1)
816 GCATTC
BsoBI 1089 CTCGGG C^[CT]CG[GA]G
2523 CCCGAG
BspDI 494 ATCGAT AT^CGAT
3069 ATCGAT
BspEI 616 TCCGGA T^CCGGA
2505 TCCGGA
Bst1107I 2700 GTATAC GTA^TAC
3667 GTATAC
BstBI 1149 TTCGAA TT^CGAA
5050 TTCGAA
BstUI 13 CGCG CG^CG
2849 CGCG
BstZ17I 2700 GTATAC GTA^TAC
3667 GTATAC
ClaI 494 ATCGAT AT^CGAT
3069 ATCGAT
DraII 1054 AGGTCCT [GA]G^G[ACGT]CC[CT]
1216 AGGCCCT
EcoO109I 1054 AGGTCCT [GA]G^G[ACGT]CC[CT]
1216 AGGCCCT
EcoRV 2015 GATATC GAT^ATC
5148 GATATC
HaeII 27 GGCGCC [GA]GCGC^[CT]
3085 GGCGCT
Hin1I 27 GGCGCC G[GA]^CG[CT]C
4805 GACGCC
MamI 2253 GATCCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3133 GATTAACATC
MluNI 320 TGGCCA TGG^CCA
3189 TGGCCA
MroI 616 TCCGGA T^CCGGA
2505 TCCGGA
MscI 320 TGGCCA TGG^CCA
3189 TGGCCA
MvnI 13 CGCG CG^CG
2849 CGCG
NspV 1149 TTCGAA TT^CGAA
5050 TTCGAA
PflFI 63 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1403 GACTTGGTC
PshBI 1262 ATTAAT AT^TAAT
4771 ATTAAT
PstI 2309 CTGCAG CTGCA^G
4923 CTGCAG
SapI 1522 GCTCTTC GCTCTTC(1/4)
2918 GCTCTTC
SfuI 1149 TTCGAA TT^CGAA
5050 TTCGAA
Tth111I 63 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1403 GACTTGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI Asp700I AspHI
AvaII BbsI BbvI BclI BfaI
BmyI BpuAI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraIII EaeI EarI
EcoRI EcoRII EcoT14I EcoT22I FauI
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MnlI MseI MslI
MspA1I MspI MunI MvaI MwoI
NdeII NlaIII NlaIV NsiI NspI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI