##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AccIII AgeI Alw44I
ApaI ApaLI AscI Asp718I AspI
AviII AvrII BglI BglII BlnI
BseAI BsgI BsiWI BspDI BspEI
BsrFI Bst1107I BstBI BstZ17I Cfr10I
ClaI CpoI DrdI EagI EclXI
Eco52I EcoRV FseI FspI HincII
HindII HpaI KpnI KspI MfeI
MluI MroI MunI NaeI NgoMIV
NheI NotI NruI NspV PacI
PflFI PinAI PvuI PvuII RsrII
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SmaI SphI Sse8387I
SwaI Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AcyI 402 GGCGCC G[GA]^CG[CT]C
AflII 4608 CTTAAG C^TTAAG
AlwNI 545 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 347 TGGCCA TGG^CCA
BbeI 402 GGCGCC GGCGC^C
BbvCI 583 GCTGAGG CCTCAGC(-5/-2)
BciVI 3960 GGATAC GTATCC(6/5)
BclI 5175 TGATCA T^GATCA
BfrI 4608 CTTAAG C^TTAAG
BlpI 2881 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2881 GCTGAGC GC^T[ACGT]AGC
BsaBI 3961 GATACGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 402 GGCGCC G[GA]^CG[CT]C
BsaWI 1983 TCCGGT [AT]^CCGG[AT]
BsiEI 1092 CGACCG CG[GA][CT]^CG
BspHI 2292 TCATGA T^CATGA
BsrDI 2478 CATTGC GCAATG(2/0)
BssHII 3727 GCGCGC G^CGCGC
BstEII 4845 GGTTACC G^GT[ACGT]ACC
BstPI 4845 GGTTACC G^GT[ACGT]ACC
CelII 2881 GCTGAGC GC^T[ACGT]AGC
DraII 2554 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 1296 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 4010 CCATGG C^C[GA][CT]GG
EcoO109I 2554 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 4845 GGTTACC G^GT[ACGT]ACC
EcoRI 1349 GAATTC G^AATTC
FbaI 5175 TGATCA T^GATCA
HgaI 4185 GACGC GACGC(5/10)
Hin1I 402 GGCGCC G[GA]^CG[CT]C
KasI 402 GGCGCC G^GCGCC
MamI 3961 GATACGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 347 TGGCCA TGG^CCA
MscI 347 TGGCCA TGG^CCA
MspA1I 3310 CAGCGG C[AC]G^C[GT]G
NarI 402 GGCGCC GG^CGCC
NcoI 4010 CCATGG C^CATGG
PaeR7I 1210 CTCGAG C^TCGAG
PflMI 5282 CCAATTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 3549 GTTTAAAC GTTT^AAAC
PpuMI 2554 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 3391 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2292 TCATGA T^CATGA
SacI 2872 GAGCTC GAGCT^C
ScaI 2540 AGTACT AGT^ACT
SfoI 402 GGCGCC GGC^GCC
SpeI 1678 ACTAGT A^CTAGT
Van91I 5282 CCAATTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1210 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 2533 GTCTAC GT^[AC][GT]AC
4241 GTAGAC
AclI 1394 AACGTT AA^CGTT
2243 AACGTT
AhdI 2404 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3355 GACTACATGTC
Aor51HI 1106 AGCGCT AGC^GCT
3629 AGCGCT
Asp700I 1392 GAAACGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2206 GAAAGTTTTC
AspEI 2404 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3355 GACTACATGTC
BamHI 1920 GGATCC G^GATCC
3966 GGATCC
BbrPI 661 CACGTG CAC^GTG
4176 CACGTG
BcgI 912 CGATGGTCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1202 GCACCCAGCTCG
BsaI 83 GGTCTC GGTCTC(1/5)
2633 GGTCTC
BsmBI 90 CGTCTC CGTCTC(1/5)
619 GAGACG
BsmI 852 GAATGC GAATGC(1/-1)
5031 GCATTC
BspLU11I 2408 ACATGT A^CATGT
3359 ACATGT
BstAPI 1251 GCACCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2425 GCATACTATGC
BstXI 602 CCAACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2477 CCATTGCTGTGG
BstYI 1920 GGATCC [GA]^GATC[CT]
3966 GGATCC
Eam1105I 2404 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3355 GACTACATGTC
Eco47III 1106 AGCGCT AGC^GCT
3629 AGCGCT
EcoNI 2490 CCTTCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4751 CCTTTGTAAGG
MflI 1920 GGATCC [GA]^GATC[CT]
3966 GGATCC
PmaCI 661 CACGTG CAC^GTG
4176 CACGTG
PmlI 661 CACGTG CAC^GTG
4176 CACGTG
Psp1406I 1394 AACGTT AA^CGTT
2243 AACGTT
PstI 209 CTGCAG CTGCA^G
2284 CTGCAG
SapI 5204 GAAGAGC GCTCTTC(1/4)
3133 GCTCTTC
SnaBI 2120 TACGTA TAC^GTA
5194 TACGTA
SspI 1963 AATATT AAT^ATT
2746 AATATT
XcmI 890 CCAAAAGAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1859 CCAGGCAAAAAATGG
XhoII 1920 GGATCC [GA]^GATC[CT]
3966 GGATCC
XmnI 1392 GAAACGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2206 GAAAGTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AocI ApoI AseI
AsnI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrBI BsrGI BsrI BssKI BssSI
BstF5I BstNI BstUI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
EaeI EarI Eco81I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MvnI MwoI NciI NdeI NdeII
NlaIII NlaIV NsiI NspI PleI
PshBI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I