##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Aor51HI ApaI AscI Asp718I
AvaI AvrII BbeI BfrI BglI
BlnI BseAI BsiWI BsmBI BsoBI
BspDI BspEI BsrFI BssHII Cfr10I
ClaI CpoI Eco47III EcoT22I FseI
KasI KpnI KspI MluI MroI
NaeI NarI NgoMIV NheI NotI
NruI NsiI PaeR7I PinAI PmeI
Psp1406I RsrII SacI SacII SbfI
SfiI SfoI SgrAI SmaI SpeI
Sse8387I SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccII 9409 CGCG CG^CG
AcyI 6422 GACGCC G[GA]^CG[CT]C
AviII 89 TGCGCA TGC^GCA
BamHI 1385 GGATCC G^GATCC
BanI 6172 GGTGCC G^G[CT][GA]CC
BbrPI 352 CACGTG CAC^GTG
BcnI 307 CCGGG CC^[GC]GG
BlpI 3846 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3846 GCTCAGC GC^T[ACGT]AGC
BsaHI 6422 GACGCC G[GA]^CG[CT]C
BsaWI 8179 TCCGGT [AT]^CCGG[AT]
BspMI 1625 ACCTGC ACCTGC(4/8)
BssSI 3933 CACGAG CACGAG(-5/-1)
BstAPI 9831 GCAAACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 9409 CGCG CG^CG
CelII 3846 GCTCAGC GC^T[ACGT]AGC
DrdI 10603 GACTCTTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 67 CGGCCG C^GGCCG
EclXI 67 CGGCCG C^GGCCG
Eco52I 67 CGGCCG C^GGCCG
EcoRI 4588 GAATTC G^AATTC
FspI 89 TGCGCA TGC^GCA
HaeII 4374 AGCGCC [GA]GCGC^[CT]
Hin1I 6422 GACGCC G[GA]^CG[CT]C
MfeI 6930 CAATTG C^AATTG
MunI 6930 CAATTG C^AATTG
MvnI 9409 CGCG CG^CG
NciI 307 CCGGG CC^[GC]GG
NcoI 5971 CCATGG C^CATGG
PacI 1476 TTAATTAA TTAAT^TAA
PflMI 9634 CCAAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 352 CACGTG CAC^GTG
PmlI 352 CACGTG CAC^GTG
PvuI 11107 CGATCG CGAT^CG
SalI 2299 GTCGAC G^TCGAC
SapI 1590 GAAGAGC GCTCTTC(1/4)
SnaBI 4886 TACGTA TAC^GTA
StuI 3896 AGGCCT AGG^CCT
Van91I 9634 CCAAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AhdI 3690 GACAAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5833 GACAGGCTGTC
Alw44I 267 GTGCAC G^TGCAC
4015 GTGCAC
ApaLI 267 GTGCAC G^TGCAC
4015 GTGCAC
AspEI 3690 GACAAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5833 GACAGGCTGTC
AspI 8990 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
9659 GACTCAGTC
BanII 1886 GAGCCC G[GA]GC[CT]^C
4984 GGGCTC
BbvCI 6642 GCTGAGG CCTCAGC(-5/-2)
10461 GCTGAGG
BsaAI 352 CACGTG [CT]AC^GT[GA]
4886 TACGTA
BsiEI 67 CGGCCG CG[GA][CT]^CG
11107 CGATCG
Bsp1407I 3007 TGTACA T^GTACA
11015 TGTACA
BsrGI 3007 TGTACA T^GTACA
11015 TGTACA
BstEII 1621 GGTCACC G^GT[ACGT]ACC
8549 GGTAACC
BstPI 1621 GGTCACC G^GT[ACGT]ACC
8549 GGTAACC
DraIII 3682 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
6760 CACACAGTG
DsaI 5971 CCATGG C^C[GA][CT]GG
8618 CCGTGG
Eam1105I 3690 GACAAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5833 GACAGGCTGTC
EcoO65I 1621 GGTCACC G^GT[ACGT]ACC
8549 GGTAACC
HapII 307 CCGG C^CGG
8180 CCGG
HgaI 5322 GACGC GACGC(5/10)
6422 GACGC
HpaI 3938 GTTAAC GTT^AAC
9179 GTTAAC
HpaII 307 CCGG C^CGG
8180 CCGG
MspI 307 CCGG C^CGG
8180 CCGG
NdeI 2364 CATATG CA^TATG
10614 CATATG
PflFI 8990 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
9659 GACTCAGTC
PshAI 4867 GACTTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5322 GACGCTAGTC
ScaI 1036 AGTACT AGT^ACT
1290 AGTACT
SphI 7238 GCATGC GCATG^C
10376 GCATGC
SspBI 3007 TGTACA T^GTACA
11015 TGTACA
Tth111I 8990 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
9659 GACTCAGTC
XcmI 2348 CCACCACCACGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
8395 CCACATATTTCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AseI AsnI Asp700I AspHI AvaII
BalI BbsI BbvI BcgI BciVI
BclI BfaI BglII BmyI BpmI
BpuAI BsaBI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspHI BspLU11I
BsrBI BsrDI BsrI BssKI Bst1107I
BstBI BstF5I BstNI BstXI BstYI
BstZ17I Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoRV EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HhaI HinP1I HincII
HindII HindIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
NspV PleI PpuMI PshBI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SfuI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XhoII XmnI