##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AviII AvrII BalI BbeI
BbrPI BbvCI BfrI BglI BlnI
BsaHI BsaI BsiEI BsiWI Bsp1407I
BspDI BspHI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstPI
BstUI BstXI Cfr10I ClaI CpoI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV FauI
FseI FspI HgaI Hin1I HincII
HindII HpaI KasI KpnI KspI
MluI MluNI MscI MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SwaI
Tth111I Van91I XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccI 1324 GTATAC GT^[AC][GT]AC
AccIII 790 TCCGGA T^CCGGA
AlwNI 102 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 365 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 4228 CTCGGG C^[CT]CG[GA]G
BamHI 3574 GGATCC G^GATCC
BcgI 681 CGAATATTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1793 GGATAC GTATCC(6/5)
BglII 2916 AGATCT A^GATCT
BlpI 3389 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3389 GCTCAGC GC^T[ACGT]AGC
BsaBI 407 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 790 TCCGGA T^CCGGA
BsmBI 4141 CGTCTC CGTCTC(1/5)
BsoBI 4228 CTCGGG C^[CT]CG[GA]G
BspEI 790 TCCGGA T^CCGGA
BspLU11I 2577 ACATGT A^CATGT
BspMI 1553 ACCTGC ACCTGC(4/8)
Bst1107I 1324 GTATAC GTA^TAC
BstAPI 2975 GCAGCACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1324 GTATAC GTA^TAC
CelII 3389 GCTCAGC GC^T[ACGT]AGC
CfoI 104 GCGC GCG^C
DrdI 3242 GACTGTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2366 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1211 GAATTC G^AATTC
HaeII 103 AGCGCC [GA]GCGC^[CT]
HhaI 104 GCGC GCG^C
HinP1I 104 GCGC G^CGC
MamI 407 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 790 TCCGGA T^CCGGA
PshAI 302 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2342 CTGCAG CTGCA^G
PvuII 4247 CAGCTG CAG^CTG
SpeI 3290 ACTAGT A^CTAGT
XmnI 365 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BanI 11 GGTGCC G^G[CT][GA]CC
3410 GGTGCC
BclI 412 TGATCA T^GATCA
1732 TGATCA
BcnI 49 CCCGG CC^[GC]GG
4210 CCCGG
BpmI 1077 CTCCAG CTGGAG(16/14)
22 CTGGAG
BsaAI 1403 TACGTG [CT]AC^GT[GA]
3114 TACGTG
BsaWI 736 ACCGGA [AT]^CCGG[AT]
790 TCCGGA
BsgI 1061 CTGCAC GTGCAG(16/14)
1202 CTGCAC
BsmI 781 GCATTC GAATGC(1/-1)
3186 GCATTC
BsrDI 1641 CATTGC GCAATG(2/0)
2852 CATTGC
DraIII 1308 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1825 CACACTGTG
EcoT14I 2503 CCAAGG C^C[AT][AT]GG
3363 CCTTGG
FbaI 412 TGATCA T^GATCA
1732 TGATCA
MfeI 838 CAATTG C^AATTG
3818 CAATTG
MslI 1118 CATATGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2035 CATATGGATG
MunI 838 CAATTG C^AATTG
3818 CAATTG
NciI 49 CCCGG CC^[GC]GG
4210 CCCGG
SacI 1288 GAGCTC GAGCT^C
4474 GAGCTC
StyI 2503 CCAAGG C^C[AT][AT]GG
3363 CCTTGG
TaqI 1342 TCGA T^CGA
4342 TCGA
TthHB8I 1342 TCGA T^CGA
4342 TCGA
XbaI 2317 TCTAGA T^CTAGA
2821 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AocI ApoI AseI AsnI
AspHI AvaII BanII BbsI BbvI
BfaI BmyI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraI DraII EarI Eco81I
EcoO109I EcoRII EcoT22I Fnu4HI FokI
HaeIII HapII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NdeI NdeII NlaIII NlaIV NsiI
NspI PleI PpuMI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StuI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII