##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AocI AscI AvrII BbrPI
BclI BfrI BglII BlnI BsaAI
BsaWI BseAI BsiEI BsmI BspDI
BspEI BspLU11I BsrDI BssHII Bst1107I
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI EagI EclXI Eco52I
Eco81I EcoO65I EcoRV EcoT22I FbaI
FseI HindIII HpaI KspI MfeI
MluI MroI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SfiI SgrAI SnaBI SpeI Sse8387I
SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 3705 GGTACC G^GTACC
AccI 3633 GTAGAC GT^[AC][GT]AC
Alw44I 809 GTGCAC G^TGCAC
Aor51HI 900 AGCGCT AGC^GCT
ApaLI 809 GTGCAC G^TGCAC
AseI 2979 ATTAAT AT^TAAT
AsnI 2979 ATTAAT AT^TAAT
Asp700I 4293 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3705 GGTACC G^GTACC
AspI 3237 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2181 TGCGCA TGC^GCA
BbvCI 3293 CCTCAGC CCTCAGC(-5/-2)
BsaBI 4073 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 490 CGTACG C^GTACG
Bsp1407I 4219 TGTACA T^GTACA
BspHI 1668 TCATGA T^CATGA
BsrGI 4219 TGTACA T^GTACA
BssSI 2378 CACGAG CACGAG(-5/-1)
BstBI 3504 TTCGAA TT^CGAA
Eco47III 900 AGCGCT AGC^GCT
EcoRI 2945 GAATTC G^AATTC
FspI 2181 TGCGCA TGC^GCA
HincII 3948 GTTGAC GT[CT]^[GA]AC
HindII 3948 GTTGAC GT[CT]^[GA]AC
KpnI 3705 GGTACC GGTAC^C
MamI 4073 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspV 3504 TTCGAA TT^CGAA
PflFI 3237 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2979 ATTAAT AT^TAAT
RcaI 1668 TCATGA T^CATGA
SexAI 1210 ACCAGGT A^CC[AT]GGT
SfuI 3504 TTCGAA TT^CGAA
SphI 2160 GCATGC GCATG^C
SspBI 4219 TGTACA T^GTACA
Tth111I 3237 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 4293 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 723 CGCG CG^CG
2215 CGCG
AhdI 1659 GACATCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4049 GACTTCAGGTC
AspEI 1659 GACATCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4049 GACTTCAGGTC
BamHI 737 GGATCC G^GATCC
4078 GGATCC
BbeI 684 GGCGCC GGCGC^C
1256 GGCGCC
BbsI 1188 GTCTTC GAAGAC(2/6)
2543 GTCTTC
BcgI 635 CGAGGGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
49 GCAGCAACCTCG
BciVI 3451 GGATAC GTATCC(6/5)
3698 GTATCC
BpuAI 1188 GTCTTC GAAGAC(2/6)
2543 GTCTTC
BsrFI 1865 GCCGGT [GA]^CCGG[CT]
2089 GCCGGT
BstUI 723 CGCG CG^CG
2215 CGCG
Cfr10I 1865 GCCGGT [GA]^CCGG[CT]
2089 GCCGGT
DrdI 1462 GACTTTCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1555 GACACTTACGTC
Eam1105I 1659 GACATCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4049 GACTTCAGGTC
EcoNI 1172 CCTCGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1736 CCTGTGAAAGG
KasI 684 GGCGCC G^GCGCC
1256 GGCGCC
MvnI 723 CGCG CG^CG
2215 CGCG
NarI 684 GGCGCC GG^CGCC
1256 GGCGCC
NspI 776 GCATGT [GA]CATG^[CT]
2160 GCATGC
PaeR7I 1173 CTCGAG C^TCGAG
1393 CTCGAG
SfoI 684 GGCGCC GGC^GCC
1256 GGCGCC
TfiI 2020 GATTC G^A[AT]TC
3049 GAATC
XhoI 1173 CTCGAG C^TCGAG
1393 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI