##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AhdI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BciVI BfrI BglII BlnI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI Eam1105I EcoRI
EcoRV FseI FspI HpaI KpnI
MfeI MluI MroI MunI NdeI
NotI NruI NspV PacI PflFI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SapI ScaI SexAI SfuI
SgrAI SnaBI SpeI SspBI Tth111I
XbaI XcmI
###################################
# ONE CUT ENZYMES ( 23 enzymes) #
###################################
AgeI 2310 ACCGGT A^CCGGT
Aor51HI 520 AGCGCT AGC^GCT
ApaI 3096 GGGCCC GGGCC^C
BcgI 4676 GCAATGATCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4342 TGATCA T^GATCA
BstEII 745 GGTCACC G^GT[ACGT]ACC
BstPI 745 GGTCACC G^GT[ACGT]ACC
DraIII 2606 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4496 GACTCTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 520 AGCGCT AGC^GCT
EcoO65I 745 GGTCACC G^GT[ACGT]ACC
FbaI 4342 TGATCA T^GATCA
HindIII 3611 AAGCTT A^AGCTT
KspI 2466 CCGCGG CCGC^GG
NcoI 3259 CCATGG C^CATGG
PinAI 2310 ACCGGT A^CCGGT
PshAI 2836 GACGGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 2466 CCGCGG CCGC^GG
SalI 2621 GTCGAC G^TCGAC
SbfI 826 CCTGCAGG CCTGCA^GG
SfiI 3097 GGCCCCCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 826 CCTGCAGG CCTGCA^GG
SwaI 5212 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 97 AAATTT [GA]^AATT[CT]
3505 AAATTT
AflIII 2118 ACGTGT A^C[GA][CT]GT
2511 ACACGT
Alw44I 2252 GTGCAC G^TGCAC
2348 GTGCAC
ApaLI 2252 GTGCAC G^TGCAC
2348 GTGCAC
ApoI 97 AAATTT [GA]^AATT[CT]
3505 AAATTT
BbvCI 4098 CCTCAGC CCTCAGC(-5/-2)
4731 CCTCAGC
BglI 3098 GCCCCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4822 GCCAGGTTGGC
BsmBI 4171 GAGACG CGTCTC(1/5)
4632 GAGACG
BstAPI 2131 GCACATCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4695 GCAACCTCTGC
BstYI 1608 AGATCC [GA]^GATC[CT]
2642 GGATCT
EagI 1756 CGGCCG C^GGCCG
2918 CGGCCG
EclXI 1756 CGGCCG C^GGCCG
2918 CGGCCG
Eco52I 1756 CGGCCG C^GGCCG
2918 CGGCCG
MflI 1608 AGATCC [GA]^GATC[CT]
2642 GGATCT
NheI 2756 GCTAGC G^CTAGC
3488 GCTAGC
PaeR7I 1967 CTCGAG C^TCGAG
2855 CTCGAG
SphI 571 GCATGC GCATG^C
1686 GCATGC
XhoI 1967 CTCGAG C^TCGAG
2855 CTCGAG
XhoII 1608 AGATCC [GA]^GATC[CT]
2642 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 149 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbeI BbrPI BbsI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstUI BstXI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NsiI NspI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XmaCI XmaI XmnI