##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AlwNI Aor51HI
ApaI AscI Asp718I AspI AviII
AvrII BalI BbeI BbvCI BcgI
BciVI BfrI BglI BlnI BlpI
Bpu1102I BsaHI BsaWI BseAI BsiWI
BsmBI BspDI BspEI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I CelII Cfr10I
ClaI CpoI DrdI DsaI Eco47III
EcoNI EcoO65I EcoRV FseI FspI
HaeII HgaI Hin1I KasI KpnI
KspI MluI MluNI MroI MscI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I StuI
Tth111I Van91I XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 28 CGCG CG^CG
AocI 92 CCTGAGG CC^T[ACGT]AGG
AseI 485 ATTAAT AT^TAAT
AsnI 485 ATTAAT AT^TAAT
AvaI 52 CCCGGG C^[CT]CG[GA]G
BamHI 1270 GGATCC G^GATCC
BbrPI 526 CACGTG CAC^GTG
BglII 3703 AGATCT A^GATCT
BsaAI 526 CACGTG [CT]AC^GT[GA]
BsaBI 1139 GATTGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 2422 CTGCAC GTGCAG(16/14)
BsiEI 30 CGGCCG CG[GA][CT]^CG
BsmFI 77 GTCCC GGGAC(10/14)
BsoBI 52 CCCGGG C^[CT]CG[GA]G
BsrBI 2819 CCGCTC CCGCTC(-3/-3)
BstUI 28 CGCG CG^CG
Bsu36I 92 CCTGAGG CC^T[ACGT]AGG
CfoI 27 GCGC GCG^C
DraII 4169 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 4564 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 30 CGGCCG C^GGCCG
EclXI 30 CGGCCG C^GGCCG
Eco52I 30 CGGCCG C^GGCCG
Eco81I 92 CCTGAGG CC^T[ACGT]AGG
EcoO109I 4169 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 2767 CCAAGG C^C[AT][AT]GG
FauI 300 CCCGC CCCGC(4/6)
HhaI 27 GCGC GCG^C
HinP1I 27 GCGC G^CGC
MamI 1139 GATTGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2806 CAATTG C^AATTG
MunI 2806 CAATTG C^AATTG
MvnI 28 CGCG CG^CG
PmaCI 526 CACGTG CAC^GTG
PmlI 526 CACGTG CAC^GTG
PshAI 3641 GACATAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 485 ATTAAT AT^TAAT
SexAI 1454 ACCTGGT A^CC[AT]GGT
SmaI 52 CCCGGG CCC^GGG
StyI 2767 CCAAGG C^C[AT][AT]GG
XbaI 2935 TCTAGA T^CTAGA
XmaCI 52 CCCGGG C^CCGGG
XmaI 52 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AhdI 50 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1197 GACACAAAGTC
Alw44I 2366 GTGCAC G^TGCAC
2451 GTGCAC
ApaLI 2366 GTGCAC G^TGCAC
2451 GTGCAC
AspEI 50 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1197 GACACAAAGTC
BanII 1028 GAGCTC G[GA]GC[CT]^C
4651 GAGCTC
BclI 1822 TGATCA T^GATCA
3743 TGATCA
BspHI 2437 TCATGA T^CATGA
4857 TCATGA
EaeI 30 CGGCCG [CT]^GGCC[GA]
2929 TGGCCG
Eam1105I 50 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1197 GACACAAAGTC
EcoRI 1189 GAATTC G^AATTC
3281 GAATTC
EcoT22I 2921 ATGCAT ATGCA^T
3793 ATGCAT
FbaI 1822 TGATCA T^GATCA
3743 TGATCA
HindIII 4336 AAGCTT A^AGCTT
4790 AAGCTT
MaeII 241 ACGT A^CGT
527 ACGT
MspA1I 84 CAGCTG C[AC]G^C[GT]G
1951 CAGCTG
NsiI 2921 ATGCAT ATGCA^T
3793 ATGCAT
PvuII 84 CAGCTG CAG^CTG
1951 CAGCTG
RcaI 2437 TCATGA T^CATGA
4857 TCATGA
SacI 1028 GAGCTC GAGCT^C
4651 GAGCTC
SapI 203 GAAGAGC GCTCTTC(1/4)
1087 GAAGAGC
SphI 373 GCATGC GCATG^C
2923 GCATGC
SwaI 1617 ATTTAAAT ATTT^AAAT
3870 ATTTAAAT
XcmI 2197 CCAGTTAACTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3174 CCACAGAAGATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AspHI AvaII
BanI BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmI Bsp1286I Bsp1407I BspLU11I BspMI
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI EarI EcoRII Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NdeI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI