##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
AspEI AviII AvrII BalI BbeI
BbrPI BcgI BciVI BfrI BglII
BlnI BsaAI BsaBI BseAI BsiEI
BsiWI BsmBI BspEI BssHII CpoI
EagI Eam1105I EclXI Eco52I EcoRI
FspI HpaI KasI KspI MamI
MfeI MluNI MroI MscI MunI
NarI NheI NotI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SbfI ScaI SfiI SfoI
SnaBI SpeI Sse8387I SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 1374 GACGTC GACGT^C
AocI 2275 CCTCAGG CC^T[ACGT]AGG
AspI 4469 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 3498 TGATCA T^GATCA
BsaWI 2547 ACCGGA [AT]^CCGG[AT]
Bsp1407I 4600 TGTACA T^GTACA
BspDI 4612 ATCGAT AT^CGAT
BspHI 4881 TCATGA T^CATGA
BspLU11I 370 ACATGT A^CATGT
BsrGI 4600 TGTACA T^GTACA
BstAPI 1116 GCATTCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3603 TTCGAA TT^CGAA
BstEII 4520 GGTGACC G^GT[ACGT]ACC
BstPI 4520 GGTGACC G^GT[ACGT]ACC
Bsu36I 2275 CCTCAGG CC^T[ACGT]AGG
ClaI 4612 ATCGAT AT^CGAT
DrdI 4469 GACAAGGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2275 CCTCAGG CC^T[ACGT]AGG
EcoO65I 4520 GGTGACC G^GT[ACGT]ACC
EcoRV 3722 GATATC GAT^ATC
EcoT22I 1399 ATGCAT ATGCA^T
FbaI 3498 TGATCA T^GATCA
FseI 2064 GGCCGGCC GGCCGG^CC
MluI 1747 ACGCGT A^CGCGT
NdeI 4248 CATATG CA^TATG
NsiI 1399 ATGCAT ATGCA^T
NspV 3603 TTCGAA TT^CGAA
PflFI 4469 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 4881 TCATGA T^CATGA
SalI 1332 GTCGAC G^TCGAC
SexAI 4527 ACCTGGT A^CC[AT]GGT
SfuI 3603 TTCGAA TT^CGAA
SgrAI 1902 CGCCGGCG C[GA]^CCGG[CT]G
SphI 603 GCATGC GCATG^C
SspBI 4600 TGTACA T^GTACA
Tth111I 4469 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 1988 GGTACC G^GTACC
2101 GGTACC
Aor51HI 1917 AGCGCT AGC^GCT
1998 AGCGCT
ApaI 2157 GGGCCC GGGCC^C
2919 GGGCCC
Asp718I 1988 GGTACC G^GTACC
2101 GGTACC
BanI 1988 GGTACC G^G[CT][GA]CC
2101 GGTACC
BbvCI 4044 CCTCAGC CCTCAGC(-5/-2)
1797 GCTGAGG
BspMI 3618 GCAGGT ACCTGC(4/8)
4070 GCAGGT
Bst1107I 84 GTATAC GTA^TAC
4105 GTATAC
BstZ17I 84 GTATAC GTA^TAC
4105 GTATAC
DsaI 1893 CCATGG C^C[GA][CT]GG
2556 CCATGG
Eco47III 1917 AGCGCT AGC^GCT
1998 AGCGCT
KpnI 1988 GGTACC GGTAC^C
2101 GGTACC
NcoI 1893 CCATGG C^CATGG
2556 CCATGG
PflMI 1224 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1546 CCAAGGATTGG
PstI 4033 CTGCAG CTGCA^G
4083 CTGCAG
SapI 3985 GAAGAGC GCTCTTC(1/4)
5006 GAAGAGC
SspI 188 AATATT AAT^ATT
319 AATATT
StuI 1821 AGGCCT AGG^CCT
4041 AGGCCT
Van91I 1224 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1546 CCAAGGATTGG
XcmI 1126 CCAAGGTGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2946 CCAAGTATCAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI Asp700I AspHI AvaI AvaII
BamHI BanII BbsI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BsrBI BsrDI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DraIII EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PleI PpuMI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmaCI XmaI
XmnI