##################################
# NO CUT ENZYMES ( 44 enzymes) #
##################################
AatII AflII AgeI AscI AseI
AsnI AviII BamHI BbeI BclI
BfrI BsiWI BspDI BssSI Bst1107I
BstBI BstZ17I ClaI DrdI EcoRV
FbaI FseI FspI KasI MfeI
MluI MunI NarI NdeI NotI
NruI NspV PacI PinAI PmeI
PshBI PvuI SfiI SfoI SfuI
SgrAI SnaBI SpeI SwaI
###################################
# ONE CUT ENZYMES ( 23 enzymes) #
###################################
AccI 3166 GTCGAC GT^[AC][GT]AC
AclI 2755 AACGTT AA^CGTT
Aor51HI 1197 AGCGCT AGC^GCT
AvrII 3046 CCTAGG C^CTAGG
BlnI 3046 CCTAGG C^CTAGG
BsaI 1734 GAGACC GGTCTC(1/5)
BspLU11I 6397 ACATGT A^CATGT
BssHII 13 GCGCGC G^CGCGC
CpoI 2330 CGGTCCG CG^G[AT]CCG
DraIII 6451 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1197 AGCGCT AGC^GCT
EcoNI 3149 CCTGGGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII 3533 AAGCTT A^AGCTT
KspI 6362 CCGCGG CCGC^GG
NaeI 642 GCCGGC GCC^GGC
NgoMIV 642 GCCGGC G^CCGGC
Psp1406I 2755 AACGTT AA^CGTT
RsrII 2330 CGGTCCG CG^G[AT]CCG
SacII 6362 CCGCGG CCGC^GG
SalI 3166 GTCGAC G^TCGAC
SbfI 1970 CCTGCAGG CCTGCA^GG
SexAI 5333 ACCAGGT A^CC[AT]GGT
Sse8387I 1970 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 424 GGTACC G^GTACC
549 GGTACC
AccIII 371 TCCGGA T^CCGGA
5130 TCCGGA
AcyI 1757 GACGCC G[GA]^CG[CT]C
3006 GACGCC
AhdI 3006 GACGCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6039 GACTGATGGTC
AlwI 404 GGATC GGATC(4/5)
1509 GGATC
AocI 148 CCTTAGG CC^T[ACGT]AGG
419 CCTGAGG
ApaI 1041 GGGCCC GGGCC^C
1964 GGGCCC
Asp700I 1271 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4900 GAAACTTTTC
Asp718I 424 GGTACC G^GTACC
549 GGTACC
AspEI 3006 GACGCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6039 GACTGATGGTC
BbvCI 3660 CCTCAGC CCTCAGC(-5/-2)
1200 GCTGAGG
BciVI 5473 GGATAC GTATCC(6/5)
595 GTATCC
BsaBI 3251 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4224 GATGTAAATC
BsaHI 1757 GACGCC G[GA]^CG[CT]C
3006 GACGCC
BseAI 371 TCCGGA T^CCGGA
5130 TCCGGA
BsiEI 640 CGGCCG CG[GA][CT]^CG
1744 CGGCCG
BspEI 371 TCCGGA T^CCGGA
5130 TCCGGA
BspHI 4212 TCATGA T^CATGA
6032 TCATGA
BstAPI 2239 GCATTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3665 GCAGAGTTTGC
BstEII 995 GGTCACC G^GT[ACGT]ACC
2628 GGTCACC
BstPI 995 GGTCACC G^GT[ACGT]ACC
2628 GGTCACC
BstXI 1156 CCAGCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4647 CCATTACAGTGG
Bsu36I 148 CCTTAGG CC^T[ACGT]AGG
419 CCTGAGG
EagI 640 CGGCCG C^GGCCG
1744 CGGCCG
Eam1105I 3006 GACGCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6039 GACTGATGGTC
EclXI 640 CGGCCG C^GGCCG
1744 CGGCCG
Eco52I 640 CGGCCG C^GGCCG
1744 CGGCCG
Eco81I 148 CCTTAGG CC^T[ACGT]AGG
419 CCTGAGG
EcoO65I 995 GGTCACC G^GT[ACGT]ACC
2628 GGTCACC
EcoRI 1415 GAATTC G^AATTC
5787 GAATTC
Hin1I 1757 GACGCC G[GA]^CG[CT]C
3006 GACGCC
HpaI 4868 GTTAAC GTT^AAC
6314 GTTAAC
KpnI 424 GGTACC GGTAC^C
549 GGTACC
MamI 3251 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4224 GATGTAAATC
MroI 371 TCCGGA T^CCGGA
5130 TCCGGA
NheI 3730 GCTAGC G^CTAGC
5622 GCTAGC
PshAI 1664 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
6088 GACTGCTGTC
RcaI 4212 TCATGA T^CATGA
6032 TCATGA
SmaI 1117 CCCGGG CCC^GGG
1177 CCCGGG
XmaCI 1117 CCCGGG C^CCGGG
1177 CCCGGG
XmaI 1117 CCCGGG C^CCGGG
1177 CCCGGG
XmnI 1271 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4900 GAAACTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 149 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwNI ApaLI ApoI
AspHI AspI AvaI AvaII BalI
BanI BanII BbrPI BbsI BbvI
BcgI BcnI BfaI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NsiI
NspI PaeR7I PflFI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SphI SspBI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII