##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccIII AcyI AhdI
Alw44I Aor51HI ApaLI AscI Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbvCI BlnI BsaAI
BsaBI BsaHI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspHI BspLU11I
BsrBI BssHII BssSI Bst1107I BstEII
BstPI BstZ17I CfoI ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoT22I FauI
FseI FspI HaeII HhaI Hin1I
HinP1I HpaI KasI KpnI KspI
MamI MluI MroI NaeI NarI
NgoMIV NotI NruI NsiI PacI
PflFI PmaCI PmeI PmlI PshAI
PvuI RcaI RsrII SacII SalI
SbfI ScaI SfoI SgrAI SmaI
SnaBI SphI Sse8387I Tth111I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 232 CGCG CG^CG
AclI 4430 AACGTT AA^CGTT
AflII 2958 CTTAAG C^TTAAG
AgeI 4154 ACCGGT A^CCGGT
AocI 5343 CCTTAGG CC^T[ACGT]AGG
ApaI 2677 GGGCCC GGGCC^C
AvaI 974 CTCGAG C^[CT]CG[GA]G
BcgI 3972 GCAGGTGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 4508 CCCGG CC^[GC]GG
BfrI 2958 CTTAAG C^TTAAG
BglI 4730 GCCATAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3147 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3147 GCTCAGC GC^T[ACGT]AGC
BsmFI 3609 GGGAC GGGAC(10/14)
BsoBI 974 CTCGAG C^[CT]CG[GA]G
Bsp1407I 5707 TGTACA T^GTACA
BsrDI 2024 GCAATG GCAATG(2/0)
BsrFI 4154 ACCGGT [GA]^CCGG[CT]
BsrGI 5707 TGTACA T^GTACA
BstUI 232 CGCG CG^CG
BstXI 4282 CCAACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 5343 CCTTAGG CC^T[ACGT]AGG
CelII 3147 GCTCAGC GC^T[ACGT]AGC
Cfr10I 4154 ACCGGT [GA]^CCGG[CT]
Eco81I 5343 CCTTAGG CC^T[ACGT]AGG
EcoNI 3883 CCTACAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 3939 GCGTC GACGC(5/10)
HindIII 3294 AAGCTT A^AGCTT
MfeI 3098 CAATTG C^AATTG
MunI 3098 CAATTG C^AATTG
MvnI 232 CGCG CG^CG
NciI 4508 CCCGG CC^[GC]GG
NspI 1274 GCATGT [GA]CATG^[CT]
PaeR7I 974 CTCGAG C^TCGAG
PinAI 4154 ACCGGT A^CCGGT
Psp1406I 4430 AACGTT AA^CGTT
SapI 3928 GAAGAGC GCTCTTC(1/4)
SfiI 4729 GGCCATAAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 3616 ACTAGT A^CTAGT
SspBI 5707 TGTACA T^GTACA
StuI 4737 AGGCCT AGG^CCT
SwaI 2666 ATTTAAAT ATTT^AAAT
XhoI 974 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 2595 GTCTAC GT^[AC][GT]AC
6794 GTCTAC
AflIII 2715 ACGTGT A^C[GA][CT]GT
5125 ACGTGT
BalI 4405 TGGCCA TGG^CCA
5921 TGGCCA
BanI 2611 GGCACC G^G[CT][GA]CC
4325 GGCACC
BciVI 968 GGATAC GTATCC(6/5)
195 GTATCC
BglII 1963 AGATCT A^GATCT
2696 AGATCT
BstAPI 877 GCAGCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2355 GCAAAGTCTGC
BstBI 1347 TTCGAA TT^CGAA
4649 TTCGAA
EcoRI 759 GAATTC G^AATTC
6649 GAATTC
EcoRV 1556 GATATC GAT^ATC
3819 GATATC
MluNI 4405 TGGCCA TGG^CCA
5921 TGGCCA
MscI 4405 TGGCCA TGG^CCA
5921 TGGCCA
NdeI 2113 CATATG CA^TATG
5314 CATATG
NheI 2011 GCTAGC G^CTAGC
2337 GCTAGC
NspV 1347 TTCGAA TT^CGAA
4649 TTCGAA
PvuII 3180 CAGCTG CAG^CTG
4412 CAGCTG
SacI 3391 GAGCTC GAGCT^C
5653 GAGCTC
SexAI 4016 ACCTGGT A^CC[AT]GGT
5974 ACCTGGT
SfuI 1347 TTCGAA TT^CGAA
4649 TTCGAA
XbaI 2256 TCTAGA T^CTAGA
6624 TCTAGA
XcmI 1897 CCAGATCCTGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5935 CCAGGGGCTAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI Asp700I
AspHI AvaII BanII BbsI BbvI
BclI BfaI BmyI BpmI BpuAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PshBI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI