##################################
# NO CUT ENZYMES ( 44 enzymes) #
##################################
AclI AgeI AscI AseI AsnI
BciVI BsaBI BsiWI BspDI BspLU11I
BssHII Bst1107I BstZ17I ClaI CpoI
DrdI EcoT22I FseI HpaI MamI
MfeI MunI NdeI NheI NotI
NruI NsiI PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SapI SbfI ScaI SfiI SgrAI
SnaBI Sse8387I SspI SwaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 6415 GACGTC GACGT^C
Acc65I 4442 GGTACC G^GTACC
AcsI 5078 GAATTC [GA]^AATT[CT]
Aor51HI 202 AGCGCT AGC^GCT
ApoI 5078 GAATTC [GA]^AATT[CT]
Asp718I 4442 GGTACC G^GTACC
AvrII 7 CCTAGG C^CTAGG
BamHI 5973 GGATCC G^GATCC
BclI 6137 TGATCA T^GATCA
BglII 5796 AGATCT A^GATCT
BlnI 7 CCTAGG C^CTAGG
Bsp1407I 1383 TGTACA T^GTACA
BspHI 1404 TCATGA T^CATGA
BsrGI 1383 TGTACA T^GTACA
BstBI 1593 TTCGAA TT^CGAA
DraI 7188 TTTAAA TTT^AAA
DraIII 1725 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 3458 CGGCCG C^GGCCG
EclXI 3458 CGGCCG C^GGCCG
Eco47III 202 AGCGCT AGC^GCT
Eco52I 3458 CGGCCG C^GGCCG
EcoRI 5078 GAATTC G^AATTC
EcoRV 3942 GATATC GAT^ATC
FbaI 6137 TGATCA T^GATCA
KpnI 4442 GGTACC GGTAC^C
MluI 4600 ACGCGT A^CGCGT
NspV 1593 TTCGAA TT^CGAA
RcaI 1404 TCATGA T^CATGA
SalI 5465 GTCGAC G^TCGAC
SexAI 1419 ACCAGGT A^CC[AT]GGT
SfuI 1593 TTCGAA TT^CGAA
SpeI 3233 ACTAGT A^CTAGT
SphI 6904 GCATGC GCATG^C
SspBI 1383 TGTACA T^GTACA
XbaI 7149 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccIII 1719 TCCGGA T^CCGGA
5976 TCCGGA
AflII 2104 CTTAAG C^TTAAG
7006 CTTAAG
AhdI 2408 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4028 GACACCCTGTC
AspEI 2408 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4028 GACACCCTGTC
AviII 1785 TGCGCA TGC^GCA
3696 TGCGCA
BfrI 2104 CTTAAG C^TTAAG
7006 CTTAAG
BglI 57 GCCCGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2171 GCCATCAAGGC
BsaI 2858 GAGACC GGTCTC(1/5)
5655 GAGACC
BsaWI 1719 TCCGGA [AT]^CCGG[AT]
5976 TCCGGA
BseAI 1719 TCCGGA T^CCGGA
5976 TCCGGA
BsiEI 3458 CGGCCG CG[GA][CT]^CG
4906 CGACCG
BspEI 1719 TCCGGA T^CCGGA
5976 TCCGGA
BstEII 938 GGTGACC G^GT[ACGT]ACC
4228 GGTGACC
BstPI 938 GGTGACC G^GT[ACGT]ACC
4228 GGTGACC
Eam1105I 2408 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4028 GACACCCTGTC
EarI 739 GAAGAG CTCTTC(1/4)
1063 GAAGAG
EcoO65I 938 GGTGACC G^GT[ACGT]ACC
4228 GGTGACC
FspI 1785 TGCGCA TGC^GCA
3696 TGCGCA
HindIII 3464 AAGCTT A^AGCTT
5723 AAGCTT
Ksp632I 739 GAAGAG CTCTTC(1/4)
1063 GAAGAG
KspI 195 CCGCGG CCGC^GG
3947 CCGCGG
MroI 1719 TCCGGA T^CCGGA
5976 TCCGGA
PaeR7I 188 CTCGAG C^TCGAG
4262 CTCGAG
SacII 195 CCGCGG CCGC^GG
3947 CCGCGG
XhoI 188 CTCGAG C^TCGAG
4262 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 154 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI Asp700I AspHI
AspI AvaI AvaII BalI BanI
BanII BbeI BbrPI BbsI BbvCI
BbvI BcgI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflFI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmaCI XmaI XmnI