##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AhdI Aor51HI AscI
AseI AsnI AspEI AspI AvrII
BamHI BbeI BbrPI BcgI BciVI
BfrI BglII BlnI BsaAI BseAI
BsiWI BsmBI BsmI BspDI BspEI
BspLU11I Bst1107I BstZ17I ClaI CpoI
Eam1105I Eco47III EcoRI EcoT22I HpaI
KasI MfeI MluI MroI MunI
NarI NheI NotI NruI NsiI
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SalI SapI SbfI ScaI
SfiI SfoI SgrAI SnaBI SpeI
SphI Sse8387I SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
Alw44I 3767 GTGCAC G^TGCAC
ApaLI 3767 GTGCAC G^TGCAC
Asp700I 1979 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI 388 CCTCAGC CCTCAGC(-5/-2)
BlpI 1713 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1713 GCTCAGC GC^T[ACGT]AGC
BsaBI 590 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2055 GAGACC GGTCTC(1/5)
BsaWI 1408 ACCGGA [AT]^CCGG[AT]
BspHI 1372 TCATGA T^CATGA
BssSI 504 CTCGTG CACGAG(-5/-1)
BstAPI 533 GCACACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 793 TTCGAA TT^CGAA
BstXI 735 CCAAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1713 GCTCAGC GC^T[ACGT]AGC
EcoRV 3519 GATATC GAT^ATC
FseI 206 GGCCGGCC GGCCGG^CC
HincII 840 GTTGAC GT[CT]^[GA]AC
HindII 840 GTTGAC GT[CT]^[GA]AC
HindIII 3856 AAGCTT A^AGCTT
KspI 212 CCGCGG CCGC^GG
MamI 590 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 3754 CATATG CA^TATG
NspV 793 TTCGAA TT^CGAA
PaeR7I 2975 CTCGAG C^TCGAG
RcaI 1372 TCATGA T^CATGA
SacII 212 CCGCGG CCGC^GG
SfuI 793 TTCGAA TT^CGAA
XhoI 2975 CTCGAG C^TCGAG
XmnI 1979 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 2038 GGTACC G^GTACC
4120 GGTACC
AcyI 4084 GACGCC G[GA]^CG[CT]C
4790 GGCGTC
ApaI 3811 GGGCCC GGGCC^C
4976 GGGCCC
Asp718I 2038 GGTACC G^GTACC
4120 GGTACC
AviII 122 TGCGCA TGC^GCA
1417 TGCGCA
BglI 182 GCCGCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
594 GCCATCTGGGC
BsaHI 4084 GACGCC G[GA]^CG[CT]C
4790 GGCGTC
Bsp1407I 662 TGTACA T^GTACA
1924 TGTACA
BsrDI 1284 GCAATG GCAATG(2/0)
2146 GCAATG
BsrGI 662 TGTACA T^GTACA
1924 TGTACA
BstEII 2874 GGTGACC G^GT[ACGT]ACC
3452 GGTCACC
BstPI 2874 GGTGACC G^GT[ACGT]ACC
3452 GGTCACC
DraIII 615 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
678 CACTATGTG
DrdI 1134 GACATCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4197 GACTTAGCTGTC
EcoO65I 2874 GGTGACC G^GT[ACGT]ACC
3452 GGTCACC
FspI 122 TGCGCA TGC^GCA
1417 TGCGCA
HaeII 99 AGCGCC [GA]GCGC^[CT]
294 GGCGCT
HgaI 4084 GACGC GACGC(5/10)
4791 GCGTC
Hin1I 4084 GACGCC G[GA]^CG[CT]C
4790 GGCGTC
KpnI 2038 GGTACC GGTAC^C
4120 GGTACC
MslI 1172 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1265 CATCCATGTG
NaeI 203 GCCGGC GCC^GGC
207 GCCGGC
NcoI 1036 CCATGG C^CATGG
2383 CCATGG
NgoMIV 203 GCCGGC G^CCGGC
207 GCCGGC
NspI 2108 ACATGC [GA]CATG^[CT]
4337 GCATGT
PflMI 2062 CCACCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2089 CCACCGACTGG
SacI 5011 GAGCTC GAGCT^C
5109 GAGCTC
SexAI 1798 ACCTGGT A^CC[AT]GGT
3430 ACCTGGT
SmaI 3052 CCCGGG CCC^GGG
3289 CCCGGG
SmlI 2975 CTCGAG C^T[CT][GA]AG
3643 CTCAAG
SspBI 662 TGTACA T^GTACA
1924 TGTACA
SspI 638 AATATT AAT^ATT
1506 AATATT
Van91I 2062 CCACCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2089 CCACCGACTGG
XbaI 3966 TCTAGA T^CTAGA
4605 TCTAGA
XcmI 496 CCACGGTGCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
580 CCAAGAGCAGGATGG
XmaCI 3052 CCCGGG C^CCGGG
3289 CCCGGG
XmaI 3052 CCCGGG C^CCGGG
3289 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BclI BcnI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssHII BssKI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EagI EarI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII