##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AclI AgeI ApaI AscI
AseI AsnI Asp700I AvrII BbrPI
BcgI BciVI BclI BlnI BsaAI
BsiWI BspDI BspHI BspLU11I BsrFI
BssHII BstBI BstEII BstPI Cfr10I
ClaI CpoI DraIII EcoNI EcoO65I
EcoRV FbaI FseI KspI MfeI
MluI MunI NaeI NdeI NgoMIV
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 3466 GGTACC G^GTACC
AccIII 550 TCCGGA T^CCGGA
AcyI 4545 GGCGCC G[GA]^CG[CT]C
AflII 4126 CTTAAG C^TTAAG
AflIII 2927 ACACGT A^C[GA][CT]GT
Alw44I 1117 GTGCAC G^TGCAC
Aor51HI 617 AGCGCT AGC^GCT
ApaLI 1117 GTGCAC G^TGCAC
Asp718I 3466 GGTACC G^GTACC
BbeI 4545 GGCGCC GGCGC^C
BbvCI 3034 GCTGAGG CCTCAGC(-5/-2)
BfrI 4126 CTTAAG C^TTAAG
BglII 3605 AGATCT A^GATCT
BlpI 114 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 114 GCTGAGC GC^T[ACGT]AGC
BsaBI 561 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 4545 GGCGCC G[GA]^CG[CT]C
BsaWI 550 TCCGGA [AT]^CCGG[AT]
BseAI 550 TCCGGA T^CCGGA
Bsp1407I 1842 TGTACA T^GTACA
BspEI 550 TCCGGA T^CCGGA
BsrBI 187 GAGCGG CCGCTC(-3/-3)
BsrGI 1842 TGTACA T^GTACA
Bst1107I 4121 GTATAC GTA^TAC
BstZ17I 4121 GTATAC GTA^TAC
CelII 114 GCTGAGC GC^T[ACGT]AGC
DrdI 1180 GACGACTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 617 AGCGCT AGC^GCT
EcoRI 757 GAATTC G^AATTC
EcoT22I 1728 ATGCAT ATGCA^T
FauI 3436 GCGGG CCCGC(4/6)
HgaI 4982 GCGTC GACGC(5/10)
Hin1I 4545 GGCGCC G[GA]^CG[CT]C
HpaI 3492 GTTAAC GTT^AAC
KasI 4545 GGCGCC G^GCGCC
KpnI 3466 GGTACC GGTAC^C
MamI 561 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 550 TCCGGA T^CCGGA
NarI 4545 GGCGCC GG^CGCC
NsiI 1728 ATGCAT ATGCA^T
PaeR7I 377 CTCGAG C^TCGAG
PstI 2325 CTGCAG CTGCA^G
SexAI 706 ACCTGGT A^CC[AT]GGT
SfoI 4545 GGCGCC GGC^GCC
SspBI 1842 TGTACA T^GTACA
XbaI 3120 TCTAGA T^CTAGA
XhoI 377 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 3449 GTAGAC GT^[AC][GT]AC
4121 GTATAC
AccII 34 CGCG CG^CG
53 CGCG
AocI 1270 CCTGAGG CC^T[ACGT]AGG
1420 CCTAAGG
AspI 1183 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4806 GACTCTGTC
AviII 228 TGCGCA TGC^GCA
246 TGCGCA
BamHI 1096 GGATCC G^GATCC
1971 GGATCC
BglI 2591 GCCATGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2981 GCCACCAAGGC
BsiEI 55 CGGCCG CG[GA][CT]^CG
125 CGGCCG
BsmBI 4650 CGTCTC CGTCTC(1/5)
671 GAGACG
BsrDI 2793 CATTGC GCAATG(2/0)
1912 GCAATG
BssSI 4354 CACGAG CACGAG(-5/-1)
4935 CTCGTG
BstAPI 1320 GCATCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4752 GCAGAGGTTGC
BstUI 34 CGCG CG^CG
53 CGCG
Bsu36I 1270 CCTGAGG CC^T[ACGT]AGG
1420 CCTAAGG
EagI 55 CGGCCG C^GGCCG
125 CGGCCG
EclXI 55 CGGCCG C^GGCCG
125 CGGCCG
Eco52I 55 CGGCCG C^GGCCG
125 CGGCCG
Eco81I 1270 CCTGAGG CC^T[ACGT]AGG
1420 CCTAAGG
FspI 228 TGCGCA TGC^GCA
246 TGCGCA
MvnI 34 CGCG CG^CG
53 CGCG
NheI 3358 GCTAGC G^CTAGC
4867 GCTAGC
PflFI 1183 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4806 GACTCTGTC
SacI 61 GAGCTC GAGCT^C
2316 GAGCTC
Tth111I 1183 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4806 GACTCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcsI AfaI AhdI AluI
AlwI AlwNI ApoI AspEI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
Eam1105I EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII