##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AgeI AocI ApaI AscI AseI
AsnI Asp718I AspI AviII AvrII
BciVI BfrI BglI BlnI BsiEI
BsiWI BsmBI Bsp1407I BsrGI BssHII
BssSI BstBI BstEII BstPI BstUI
Bsu36I CpoI DrdI EagI EclXI
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FauI FseI FspI HgaI KpnI
KspI MfeI MluI MunI MvnI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacII SalI SbfI SfiI
SfuI SgrAI SmaI SnaBI Sse8387I
SspBI StuI SwaI Tth111I XbaI
XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 592 GTATAC GT^[AC][GT]AC
AccIII 162 TCCGGA T^CCGGA
AcyI 3191 GGCGCC G[GA]^CG[CT]C
AhdI 4844 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3382 GTGCAC G^TGCAC
Aor51HI 207 AGCGCT AGC^GCT
ApaLI 3382 GTGCAC G^TGCAC
AspEI 4844 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 4799 CTCGAG C^[CT]CG[GA]G
BalI 1555 TGGCCA TGG^CCA
BamHI 2647 GGATCC G^GATCC
BbeI 3191 GGCGCC GGCGC^C
BbrPI 423 CACGTG CAC^GTG
BbvCI 2654 CCTCAGC CCTCAGC(-5/-2)
BcgI 3259 CGAAGCTCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 179 CCCGG CC^[GC]GG
BsaHI 3191 GGCGCC G[GA]^CG[CT]C
BsaWI 162 TCCGGA [AT]^CCGG[AT]
BseAI 162 TCCGGA T^CCGGA
BsmI 918 GCATTC GAATGC(1/-1)
BsoBI 4799 CTCGAG C^[CT]CG[GA]G
BspDI 2849 ATCGAT AT^CGAT
BspEI 162 TCCGGA T^CCGGA
BspLU11I 4709 ACATGT A^CATGT
BsrBI 101 GAGCGG CCGCTC(-3/-3)
Bst1107I 592 GTATAC GTA^TAC
BstXI 1098 CCAGAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 592 GTATAC GTA^TAC
ClaI 2849 ATCGAT AT^CGAT
EaeI 1555 TGGCCA [CT]^GGCC[GA]
Eam1105I 4844 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 207 AGCGCT AGC^GCT
EcoNI 4100 CCTAGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1636 GAATTC G^AATTC
Hin1I 3191 GGCGCC G[GA]^CG[CT]C
HpaI 3254 GTTAAC GTT^AAC
KasI 3191 GGCGCC G^GCGCC
MluNI 1555 TGGCCA TGG^CCA
MroI 162 TCCGGA T^CCGGA
MscI 1555 TGGCCA TGG^CCA
NarI 3191 GGCGCC GG^CGCC
NciI 179 CCCGG CC^[GC]GG
PaeR7I 4799 CTCGAG C^TCGAG
PmaCI 423 CACGTG CAC^GTG
PmlI 423 CACGTG CAC^GTG
PpuMI 3316 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 72 GAGCTC GAGCT^C
ScaI 1726 AGTACT AGT^ACT
SexAI 4663 ACCAGGT A^CC[AT]GGT
SfoI 3191 GGCGCC GGC^GCC
XhoI 4799 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
BanI 3191 GGCGCC G^G[CT][GA]CC
3854 GGCACC
BclI 952 TGATCA T^GATCA
1924 TGATCA
BglII 1624 AGATCT A^GATCT
1670 AGATCT
BlpI 210 GCTTAGC GC^T[ACGT]AGC
3806 GCTCAGC
Bpu1102I 210 GCTTAGC GC^T[ACGT]AGC
3806 GCTCAGC
BsaAI 423 CACGTG [CT]AC^GT[GA]
4614 TACGTG
BsaBI 3482 GATTCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4670 GATATGGATC
BsrFI 3370 GCCGGC [GA]^CCGG[CT]
3851 GCCGGC
CelII 210 GCTTAGC GC^T[ACGT]AGC
3806 GCTCAGC
CfoI 208 GCGC GCG^C
3192 GCGC
Cfr10I 3370 GCCGGC [GA]^CCGG[CT]
3851 GCCGGC
DraII 3316 AGGTCCT [GA]G^G[ACGT]CC[CT]
3861 AGGCCCC
DraIII 735 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
4662 CACCAGGTG
EcoO109I 3316 AGGTCCT [GA]G^G[ACGT]CC[CT]
3861 AGGCCCC
FbaI 952 TGATCA T^GATCA
1924 TGATCA
HaeII 207 AGCGCT [GA]GCGC^[CT]
3191 GGCGCC
HhaI 208 GCGC GCG^C
3192 GCGC
HinP1I 208 GCGC G^CGC
3192 GCGC
HindIII 1616 AAGCTT A^AGCTT
1646 AAGCTT
MamI 3482 GATTCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4670 GATATGGATC
NaeI 3370 GCCGGC GCC^GGC
3851 GCCGGC
NcoI 483 CCATGG C^CATGG
3680 CCATGG
NgoMIV 3370 GCCGGC G^CCGGC
3851 GCCGGC
PflMI 2513 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3675 CCAAGCCATGG
PstI 768 CTGCAG CTGCA^G
1872 CTGCAG
SpeI 404 ACTAGT A^CTAGT
1064 ACTAGT
SphI 648 GCATGC GCATG^C
2076 GCATGC
SspI 2400 AATATT AAT^ATT
4994 AATATT
Van91I 2513 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3675 CCAAGCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DsaI EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
RcaI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI